Evaluation of the effects of dexmedetomidine and midazolam on plasma and cerebrospinal fluid beta-endorphin levels in rats
Endorphins are endogenous peptides that play a role in the regulation of pain, behavior, neuroendocrine and autonomic functions. In our study, we aimed to investigate the role of β endorphins in these effects of midazolam and dexmedetomidine. In this experimental study, 36 rats were divided...
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| Format: | Article |
| Language: | English |
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Society of Turaz Bilim
2021-09-01
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| Series: | Medicine Science |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=59830 |
| _version_ | 1797328535072800768 |
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| author | Canan Kucuk Nihal Kadiogullari |
| author_facet | Canan Kucuk Nihal Kadiogullari |
| author_sort | Canan Kucuk |
| collection | DOAJ |
| description | Endorphins are endogenous peptides that play a role in the regulation of pain, behavior, neuroendocrine and autonomic functions. In our study, we aimed to investigate the role of β endorphins in these effects of midazolam and dexmedetomidine. In this experimental study, 36 rats were divided into 6 groups. Group 1: (control) (n=6): An average of 100-150μl CSF samples were obtained after general anesthesia was achieved. An intracardiac 3-4ml blood sample was then taken. Group 2: (sham) (n=6): intraperitoneal 1 mL saline was administered. For all other groups after general anesthesia; Group 3: (midazolam-1) (n=6): intraperitoneal 4mg/kg midazolam was administered. Group 4: (midazolam-2) (n=6): 12mg/kg midazolam was administered intraperitoneally. Group 5: (Dexmedetomidine-1) (n=6): Dexmedetomidine 50µg/kg was administered intraperitoneally. Group 6: (Dexmedetomidine-2) (n=6): Dexmedetomidine 150 µg/kg was administered intraperitoneally.CSF and plasma samples were taken with the same method. All samples were stored at -80 °C. No statistically significant difference was found between the levels of rat CSF β-endorphin. However, when the plasma ß-endorphin levels of rats were evaluated, there was a statistically significant difference between the groups. No difference was found between the levels of plasma ß-endorphin in the patients who received midazolam, while plasma ß-endorphin levels of 50 µg/kg with dexmedetomidine were found to be lower than the control group. However, the values of the 150 µg/kg dexmedetomidine group did not support this result. Different findings from both this study and in vitro and human studies; the number of subjects, the use of different drug doses, the differences in measurement times, the absence of control groups may be due to methodological differences, and more research is needed. [Med-Science 2021; 10(3.000): 737-40] |
| first_indexed | 2024-03-08T06:53:09Z |
| format | Article |
| id | doaj.art-95a8d1f44f9c47d59afc5721babeaf4e |
| institution | Directory Open Access Journal |
| issn | 2147-0634 |
| language | English |
| last_indexed | 2024-03-08T06:53:09Z |
| publishDate | 2021-09-01 |
| publisher | Society of Turaz Bilim |
| record_format | Article |
| series | Medicine Science |
| spelling | doaj.art-95a8d1f44f9c47d59afc5721babeaf4e2024-02-03T06:40:24ZengSociety of Turaz BilimMedicine Science2147-06342021-09-011037374010.5455/medscience.2021.03.08159830Evaluation of the effects of dexmedetomidine and midazolam on plasma and cerebrospinal fluid beta-endorphin levels in ratsCanan Kucuk0Nihal Kadiogullari129 Mayis Hospital, Aydinlar, Dikmen Cd. No312, 06105 CankayaAnkara Dr. Abdurrahman Yurtaslan Ankara Onkoloji Egitim ve Arastirma Hastanesi, Vatan Cd., 06200 YenimahalleAnkaraEndorphins are endogenous peptides that play a role in the regulation of pain, behavior, neuroendocrine and autonomic functions. In our study, we aimed to investigate the role of β endorphins in these effects of midazolam and dexmedetomidine. In this experimental study, 36 rats were divided into 6 groups. Group 1: (control) (n=6): An average of 100-150μl CSF samples were obtained after general anesthesia was achieved. An intracardiac 3-4ml blood sample was then taken. Group 2: (sham) (n=6): intraperitoneal 1 mL saline was administered. For all other groups after general anesthesia; Group 3: (midazolam-1) (n=6): intraperitoneal 4mg/kg midazolam was administered. Group 4: (midazolam-2) (n=6): 12mg/kg midazolam was administered intraperitoneally. Group 5: (Dexmedetomidine-1) (n=6): Dexmedetomidine 50µg/kg was administered intraperitoneally. Group 6: (Dexmedetomidine-2) (n=6): Dexmedetomidine 150 µg/kg was administered intraperitoneally.CSF and plasma samples were taken with the same method. All samples were stored at -80 °C. No statistically significant difference was found between the levels of rat CSF β-endorphin. However, when the plasma ß-endorphin levels of rats were evaluated, there was a statistically significant difference between the groups. No difference was found between the levels of plasma ß-endorphin in the patients who received midazolam, while plasma ß-endorphin levels of 50 µg/kg with dexmedetomidine were found to be lower than the control group. However, the values of the 150 µg/kg dexmedetomidine group did not support this result. Different findings from both this study and in vitro and human studies; the number of subjects, the use of different drug doses, the differences in measurement times, the absence of control groups may be due to methodological differences, and more research is needed. [Med-Science 2021; 10(3.000): 737-40]http://www.ejmanager.com/fulltextpdf.php?mno=59830β-endorphinmidazolamdexmedetomidine |
| spellingShingle | Canan Kucuk Nihal Kadiogullari Evaluation of the effects of dexmedetomidine and midazolam on plasma and cerebrospinal fluid beta-endorphin levels in rats Medicine Science β-endorphin midazolam dexmedetomidine |
| title | Evaluation of the effects of dexmedetomidine and midazolam on plasma and cerebrospinal fluid beta-endorphin levels in rats |
| title_full | Evaluation of the effects of dexmedetomidine and midazolam on plasma and cerebrospinal fluid beta-endorphin levels in rats |
| title_fullStr | Evaluation of the effects of dexmedetomidine and midazolam on plasma and cerebrospinal fluid beta-endorphin levels in rats |
| title_full_unstemmed | Evaluation of the effects of dexmedetomidine and midazolam on plasma and cerebrospinal fluid beta-endorphin levels in rats |
| title_short | Evaluation of the effects of dexmedetomidine and midazolam on plasma and cerebrospinal fluid beta-endorphin levels in rats |
| title_sort | evaluation of the effects of dexmedetomidine and midazolam on plasma and cerebrospinal fluid beta endorphin levels in rats |
| topic | β-endorphin midazolam dexmedetomidine |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=59830 |
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