Construction of a Yeast Cell-Based Assay System to Analyze SNAP25-Targeting Botulinum Neurotoxins

Construction of a Yeast Cell-Based Assay System to Analyze SNAP25-Targeting Botulinum Neurotoxins

Herein, we describe a yeast cell-based assay system to analyze SNAP25-targeting botulinum neurotoxins (BoNTs). BoNTs are protein toxins, and, upon incorporation into neuronal cells, their light chains (BoNT-LCs) target specific synaptosomal N-ethylmaleimide-sensitive attachment protein receptor (SNA...

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Bibliographic Details
Main Authors: Shilin Chen, Feng Li, Guoyu Liu, Yuqing Li, Zijie Li, Yishi Liu, Hideki Nakanishi
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Microorganisms
Subjects:
botulinum neurotoxins
yeast cell-based assay
SNARE
SNAP25
sporulation
Online Access:https://www.mdpi.com/2076-2607/11/5/1125

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