First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina
We describe the first isolation of an enteroaggregative Escherichia coli (EAEC) O104:H4 strain associated with an acute diarrhea case in Argentina. Two multiplex PCRs (mPCR) were performed as screening of genes mPCR1 (eae, lt, and st) and mPCR2 (IpaH, aggR, stx1 and stx2). A mPCR to detect the rfbO1...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier España
2014-12-01
|
| Series: | Revista Argentina de Microbiología |
| Subjects: | |
| Online Access: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412014000500004&lng=en&tlng=en |
| _version_ | 1818161064097873920 |
|---|---|
| author | Claudia C Carbonari Natalia Deza Mario Flores Alejandra Gasparini Eduardo Manfredi Marta Rivas |
| author_facet | Claudia C Carbonari Natalia Deza Mario Flores Alejandra Gasparini Eduardo Manfredi Marta Rivas |
| author_sort | Claudia C Carbonari |
| collection | DOAJ |
| description | We describe the first isolation of an enteroaggregative Escherichia coli (EAEC) O104:H4 strain associated with an acute diarrhea case in Argentina. Two multiplex PCRs (mPCR) were performed as screening of genes mPCR1 (eae, lt, and st) and mPCR2 (IpaH, aggR, stx1 and stx2). A mPCR to detect the rfbO104, fliC H4 and terD genes, and PCR assays for the detection of pCVD432 plasmid, aaiC and lpfO113 genes were included. Biochemical and antimicrobial susceptibility assays as well as serotyping were performed. The identified E. coli strain was susceptible to all antimicrobials tested and harbored the aggR, aaiC, pCVD432 plasmid, lpfO113, rfbO104, fliC H4 and terD genes. Although serotype EAEC O104:H4 rarely spreads and sporadic cases have been reported, global concern increased after the large-scale outbreak in Europe in 2011. The finding of EAEC O104:H4 reinforces the need for improved methodologies for the detection of all E. coli pathotypes. |
| first_indexed | 2024-12-11T16:11:49Z |
| format | Article |
| id | doaj.art-95a944f839f541cd901e8c999623c90a |
| institution | Directory Open Access Journal |
| issn | 1851-7617 |
| language | English |
| last_indexed | 2024-12-11T16:11:49Z |
| publishDate | 2014-12-01 |
| publisher | Elsevier España |
| record_format | Article |
| series | Revista Argentina de Microbiología |
| spelling | doaj.art-95a944f839f541cd901e8c999623c90a2022-12-22T00:59:03ZengElsevier EspañaRevista Argentina de Microbiología1851-76172014-12-01464302306S0325-75412014000500004First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in ArgentinaClaudia C Carbonari0Natalia Deza1Mario Flores2Alejandra Gasparini3Eduardo Manfredi4Marta Rivas5ANLIS Dr. Carlos G. MalbránANLIS Dr. Carlos G. MalbránProvincia de ChubutHospital Zonal de TrelewANLIS Dr. Carlos G. MalbránANLIS Dr. Carlos G. MalbránWe describe the first isolation of an enteroaggregative Escherichia coli (EAEC) O104:H4 strain associated with an acute diarrhea case in Argentina. Two multiplex PCRs (mPCR) were performed as screening of genes mPCR1 (eae, lt, and st) and mPCR2 (IpaH, aggR, stx1 and stx2). A mPCR to detect the rfbO104, fliC H4 and terD genes, and PCR assays for the detection of pCVD432 plasmid, aaiC and lpfO113 genes were included. Biochemical and antimicrobial susceptibility assays as well as serotyping were performed. The identified E. coli strain was susceptible to all antimicrobials tested and harbored the aggR, aaiC, pCVD432 plasmid, lpfO113, rfbO104, fliC H4 and terD genes. Although serotype EAEC O104:H4 rarely spreads and sporadic cases have been reported, global concern increased after the large-scale outbreak in Europe in 2011. The finding of EAEC O104:H4 reinforces the need for improved methodologies for the detection of all E. coli pathotypes.http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412014000500004&lng=en&tlng=enEscherichia coli enteroagregativoSerotipo O104:H4Diarrea |
| spellingShingle | Claudia C Carbonari Natalia Deza Mario Flores Alejandra Gasparini Eduardo Manfredi Marta Rivas First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina Revista Argentina de Microbiología Escherichia coli enteroagregativo Serotipo O104:H4 Diarrea |
| title | First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina |
| title_full | First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina |
| title_fullStr | First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina |
| title_full_unstemmed | First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina |
| title_short | First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina |
| title_sort | first isolation of enteroaggregative escherichia coli o104 h4 from a diarrhea case in argentina |
| topic | Escherichia coli enteroagregativo Serotipo O104:H4 Diarrea |
| url | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412014000500004&lng=en&tlng=en |
| work_keys_str_mv | AT claudiaccarbonari firstisolationofenteroaggregativeescherichiacolio104h4fromadiarrheacaseinargentina AT nataliadeza firstisolationofenteroaggregativeescherichiacolio104h4fromadiarrheacaseinargentina AT marioflores firstisolationofenteroaggregativeescherichiacolio104h4fromadiarrheacaseinargentina AT alejandragasparini firstisolationofenteroaggregativeescherichiacolio104h4fromadiarrheacaseinargentina AT eduardomanfredi firstisolationofenteroaggregativeescherichiacolio104h4fromadiarrheacaseinargentina AT martarivas firstisolationofenteroaggregativeescherichiacolio104h4fromadiarrheacaseinargentina |