Reduced Expression of Septin7 Hinders Skeletal Muscle Regeneration
Septins are considered the fourth component of the cytoskeleton with the septin7 isoform playing a critical role in the formation of diffusion barriers in phospholipid bilayers and intra- and extracellular scaffolds. While its importance has already been confirmed in different intracellular processe...
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/17/13536 |
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| author | László Szabó Andrea Telek János Fodor Nóra Dobrosi Klaudia Dócs Zoltán Hegyi Mónika Gönczi László Csernoch Beatrix Dienes |
| author_facet | László Szabó Andrea Telek János Fodor Nóra Dobrosi Klaudia Dócs Zoltán Hegyi Mónika Gönczi László Csernoch Beatrix Dienes |
| author_sort | László Szabó |
| collection | DOAJ |
| description | Septins are considered the fourth component of the cytoskeleton with the septin7 isoform playing a critical role in the formation of diffusion barriers in phospholipid bilayers and intra- and extracellular scaffolds. While its importance has already been confirmed in different intracellular processes, very little is known about its role in skeletal muscle. Muscle regeneration was studied in a <i>Sept7</i> conditional knock-down mouse model to prove the possible role of septin7 in this process. Sterile inflammation in skeletal muscle was induced which was followed by regeneration resulting in the upregulation of septin7 expression. Partial knock-down of <i>Sept7</i> resulted in an increased number of inflammatory cells and myofibers containing central nuclei. Taken together, our data suggest that partial knock-down of <i>Sept7</i> hinders the kinetics of muscle regeneration, indicating its crucial role in skeletal muscle functions. |
| first_indexed | 2024-03-10T23:20:41Z |
| format | Article |
| id | doaj.art-95aab52f9e6345bea6a79bded66f4fc4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:20:41Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-95aab52f9e6345bea6a79bded66f4fc42023-11-19T08:18:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124171353610.3390/ijms241713536Reduced Expression of Septin7 Hinders Skeletal Muscle RegenerationLászló Szabó0Andrea Telek1János Fodor2Nóra Dobrosi3Klaudia Dócs4Zoltán Hegyi5Mónika Gönczi6László Csernoch7Beatrix Dienes8Department of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Anatomy, Histology and Embryology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungarySeptins are considered the fourth component of the cytoskeleton with the septin7 isoform playing a critical role in the formation of diffusion barriers in phospholipid bilayers and intra- and extracellular scaffolds. While its importance has already been confirmed in different intracellular processes, very little is known about its role in skeletal muscle. Muscle regeneration was studied in a <i>Sept7</i> conditional knock-down mouse model to prove the possible role of septin7 in this process. Sterile inflammation in skeletal muscle was induced which was followed by regeneration resulting in the upregulation of septin7 expression. Partial knock-down of <i>Sept7</i> resulted in an increased number of inflammatory cells and myofibers containing central nuclei. Taken together, our data suggest that partial knock-down of <i>Sept7</i> hinders the kinetics of muscle regeneration, indicating its crucial role in skeletal muscle functions.https://www.mdpi.com/1422-0067/24/17/13536septin7skeletal muscleregenerationcentral nucleimuscle injury |
| spellingShingle | László Szabó Andrea Telek János Fodor Nóra Dobrosi Klaudia Dócs Zoltán Hegyi Mónika Gönczi László Csernoch Beatrix Dienes Reduced Expression of Septin7 Hinders Skeletal Muscle Regeneration International Journal of Molecular Sciences septin7 skeletal muscle regeneration central nuclei muscle injury |
| title | Reduced Expression of Septin7 Hinders Skeletal Muscle Regeneration |
| title_full | Reduced Expression of Septin7 Hinders Skeletal Muscle Regeneration |
| title_fullStr | Reduced Expression of Septin7 Hinders Skeletal Muscle Regeneration |
| title_full_unstemmed | Reduced Expression of Septin7 Hinders Skeletal Muscle Regeneration |
| title_short | Reduced Expression of Septin7 Hinders Skeletal Muscle Regeneration |
| title_sort | reduced expression of septin7 hinders skeletal muscle regeneration |
| topic | septin7 skeletal muscle regeneration central nuclei muscle injury |
| url | https://www.mdpi.com/1422-0067/24/17/13536 |
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