The complex architecture and epigenomic impact of plant T-DNA insertions.
The bacterium Agrobacterium tumefaciens has been the workhorse in plant genome engineering. Customized replacement of native tumor-inducing (Ti) plasmid elements enabled insertion of a sequence of interest called Transfer-DNA (T-DNA) into any plant genome. Although these transfer mechanisms are well...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1007819 |
| _version_ | 1818581282369568768 |
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| author | Florian Jupe Angeline C Rivkin Todd P Michael Mark Zander S Timothy Motley Justin P Sandoval R Keith Slotkin Huaming Chen Rosa Castanon Joseph R Nery Joseph R Ecker |
| author_facet | Florian Jupe Angeline C Rivkin Todd P Michael Mark Zander S Timothy Motley Justin P Sandoval R Keith Slotkin Huaming Chen Rosa Castanon Joseph R Nery Joseph R Ecker |
| author_sort | Florian Jupe |
| collection | DOAJ |
| description | The bacterium Agrobacterium tumefaciens has been the workhorse in plant genome engineering. Customized replacement of native tumor-inducing (Ti) plasmid elements enabled insertion of a sequence of interest called Transfer-DNA (T-DNA) into any plant genome. Although these transfer mechanisms are well understood, detailed understanding of structure and epigenomic status of insertion events was limited by current technologies. Here we applied two single-molecule technologies and analyzed Arabidopsis thaliana lines from three widely used T-DNA insertion collections (SALK, SAIL and WISC). Optical maps for four randomly selected T-DNA lines revealed between one and seven insertions/rearrangements, and the length of individual insertions from 27 to 236 kilobases. De novo nanopore sequencing-based assemblies for two segregating lines partially resolved T-DNA structures and revealed multiple translocations and exchange of chromosome arm ends. For the current TAIR10 reference genome, nanopore contigs corrected 83% of non-centromeric misassemblies. The unprecedented contiguous nucleotide-level resolution enabled an in-depth study of the epigenome at T-DNA insertion sites. SALK_059379 line T-DNA insertions were enriched for 24nt small interfering RNAs (siRNA) and dense cytosine DNA methylation, resulting in transgene silencing via the RNA-directed DNA methylation pathway. In contrast, SAIL_232 line T-DNA insertions are predominantly targeted by 21/22nt siRNAs, with DNA methylation and silencing limited to a reporter, but not the resistance gene. Additionally, we profiled the H3K4me3, H3K27me3 and H2A.Z chromatin environments around T-DNA insertions using ChIP-seq in SALK_059379, SAIL_232 and five additional T-DNA lines. We discovered various effect s ranging from complete loss of chromatin marks to the de novo incorporation of H2A.Z and trimethylation of H3K4 and H3K27 around the T-DNA integration sites. This study provides new insights into the structural impact of inserting foreign fragments into plant genomes and demonstrates the utility of state-of-the-art long-range sequencing technologies to rapidly identify unanticipated genomic changes. |
| first_indexed | 2024-12-16T07:31:01Z |
| format | Article |
| id | doaj.art-95ab8fb90c2041d8ab9f191a2f75b785 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-16T07:31:01Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-95ab8fb90c2041d8ab9f191a2f75b7852022-12-21T22:39:21ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042019-01-01151e100781910.1371/journal.pgen.1007819The complex architecture and epigenomic impact of plant T-DNA insertions.Florian JupeAngeline C RivkinTodd P MichaelMark ZanderS Timothy MotleyJustin P SandovalR Keith SlotkinHuaming ChenRosa CastanonJoseph R NeryJoseph R EckerThe bacterium Agrobacterium tumefaciens has been the workhorse in plant genome engineering. Customized replacement of native tumor-inducing (Ti) plasmid elements enabled insertion of a sequence of interest called Transfer-DNA (T-DNA) into any plant genome. Although these transfer mechanisms are well understood, detailed understanding of structure and epigenomic status of insertion events was limited by current technologies. Here we applied two single-molecule technologies and analyzed Arabidopsis thaliana lines from three widely used T-DNA insertion collections (SALK, SAIL and WISC). Optical maps for four randomly selected T-DNA lines revealed between one and seven insertions/rearrangements, and the length of individual insertions from 27 to 236 kilobases. De novo nanopore sequencing-based assemblies for two segregating lines partially resolved T-DNA structures and revealed multiple translocations and exchange of chromosome arm ends. For the current TAIR10 reference genome, nanopore contigs corrected 83% of non-centromeric misassemblies. The unprecedented contiguous nucleotide-level resolution enabled an in-depth study of the epigenome at T-DNA insertion sites. SALK_059379 line T-DNA insertions were enriched for 24nt small interfering RNAs (siRNA) and dense cytosine DNA methylation, resulting in transgene silencing via the RNA-directed DNA methylation pathway. In contrast, SAIL_232 line T-DNA insertions are predominantly targeted by 21/22nt siRNAs, with DNA methylation and silencing limited to a reporter, but not the resistance gene. Additionally, we profiled the H3K4me3, H3K27me3 and H2A.Z chromatin environments around T-DNA insertions using ChIP-seq in SALK_059379, SAIL_232 and five additional T-DNA lines. We discovered various effect s ranging from complete loss of chromatin marks to the de novo incorporation of H2A.Z and trimethylation of H3K4 and H3K27 around the T-DNA integration sites. This study provides new insights into the structural impact of inserting foreign fragments into plant genomes and demonstrates the utility of state-of-the-art long-range sequencing technologies to rapidly identify unanticipated genomic changes.https://doi.org/10.1371/journal.pgen.1007819 |
| spellingShingle | Florian Jupe Angeline C Rivkin Todd P Michael Mark Zander S Timothy Motley Justin P Sandoval R Keith Slotkin Huaming Chen Rosa Castanon Joseph R Nery Joseph R Ecker The complex architecture and epigenomic impact of plant T-DNA insertions. PLoS Genetics |
| title | The complex architecture and epigenomic impact of plant T-DNA insertions. |
| title_full | The complex architecture and epigenomic impact of plant T-DNA insertions. |
| title_fullStr | The complex architecture and epigenomic impact of plant T-DNA insertions. |
| title_full_unstemmed | The complex architecture and epigenomic impact of plant T-DNA insertions. |
| title_short | The complex architecture and epigenomic impact of plant T-DNA insertions. |
| title_sort | complex architecture and epigenomic impact of plant t dna insertions |
| url | https://doi.org/10.1371/journal.pgen.1007819 |
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