CCL2 enhances the viability of human chorionic trophoblast cell line HTR-8/SVneo Cells by inhibiting Interleukin-24 Expression
Background: To investigate the regulatory effect of interleukin-24 (IL-24) on cell viability of human chorionic trophoblast cell line (HTR-8/SVneo cells). Methods: Immunohistochemical staining was used to detect the expression of IL-24 and its receptors IL-20R1, IL-20R2, and IL-22R1 in villus tissue...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health/LWW
2017-01-01
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| Series: | Reproductive and Developmental Medicine |
| Subjects: | |
| Online Access: | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2017;volume=1;issue=4;spage=198;epage=203;aulast=Shao |
| _version_ | 1818523309069828096 |
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| author | Jun Shao Yin-Yan He Da-Jin Li Ming-Qing Li |
| author_facet | Jun Shao Yin-Yan He Da-Jin Li Ming-Qing Li |
| author_sort | Jun Shao |
| collection | DOAJ |
| description | Background: To investigate the regulatory effect of interleukin-24 (IL-24) on cell viability of human chorionic trophoblast cell line (HTR-8/SVneo cells).
Methods: Immunohistochemical staining was used to detect the expression of IL-24 and its receptors IL-20R1, IL-20R2, and IL-22R1 in villus tissue at early normal pregnancy. The effect of thymic stromal lymphopoietin (TSLP) and chemokine CCL2 on the expression of IL-24 in human chorionic trophoblast cell line HTR-8/SVneo cells was analyzed by In-cell Western. In addition, the effect of recombinant human IL-24 (rhIL-24) and CCL2 on the viability of HTR-8/SVneo cells was analyzed by MTT assay.
Results: IL-24 and its receptors showed a strong positive staining in trophoblasts at early normal pregnancy. Compared with control group, expression of IL-24 in HTR-8/SVneo cells was significantly inhibited after in vitro stimulation of recombinant human CCL2 protein (rhCCL2) (P < 0.001). The viability of HTR-8/SVneo cells was significantly decreased after treatment with rhIL-24 (P < 0.001). In contrast, anti-IL-24 neutralizing antibody significantly enhanced the viability of HTR-8/SVneo cells (P < 0.01). In addition, rhCCL2 (100 μg/L); enhanced the viability of HTR-8/SVneo cells (P < 0.01) in vitro, but this effect was inhibited by treatment with rhIL-24.
Conclusions: CCL2 enhances the viability of human trophoblast cell line HTR-8/SVneo cells in vitro by inhibiting the secretion of IL-24, which may be beneficial to blastocyst implantation and placental development. |
| first_indexed | 2024-12-11T05:43:26Z |
| format | Article |
| id | doaj.art-95abeec0f430438dbff296362852c528 |
| institution | Directory Open Access Journal |
| issn | 2096-2924 |
| language | English |
| last_indexed | 2024-12-11T05:43:26Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Health/LWW |
| record_format | Article |
| series | Reproductive and Developmental Medicine |
| spelling | doaj.art-95abeec0f430438dbff296362852c5282022-12-22T01:19:04ZengWolters Kluwer Health/LWWReproductive and Developmental Medicine2096-29242017-01-011419820310.4103/2096-2924.224918CCL2 enhances the viability of human chorionic trophoblast cell line HTR-8/SVneo Cells by inhibiting Interleukin-24 ExpressionJun ShaoYin-Yan HeDa-Jin LiMing-Qing LiBackground: To investigate the regulatory effect of interleukin-24 (IL-24) on cell viability of human chorionic trophoblast cell line (HTR-8/SVneo cells). Methods: Immunohistochemical staining was used to detect the expression of IL-24 and its receptors IL-20R1, IL-20R2, and IL-22R1 in villus tissue at early normal pregnancy. The effect of thymic stromal lymphopoietin (TSLP) and chemokine CCL2 on the expression of IL-24 in human chorionic trophoblast cell line HTR-8/SVneo cells was analyzed by In-cell Western. In addition, the effect of recombinant human IL-24 (rhIL-24) and CCL2 on the viability of HTR-8/SVneo cells was analyzed by MTT assay. Results: IL-24 and its receptors showed a strong positive staining in trophoblasts at early normal pregnancy. Compared with control group, expression of IL-24 in HTR-8/SVneo cells was significantly inhibited after in vitro stimulation of recombinant human CCL2 protein (rhCCL2) (P < 0.001). The viability of HTR-8/SVneo cells was significantly decreased after treatment with rhIL-24 (P < 0.001). In contrast, anti-IL-24 neutralizing antibody significantly enhanced the viability of HTR-8/SVneo cells (P < 0.01). In addition, rhCCL2 (100 μg/L); enhanced the viability of HTR-8/SVneo cells (P < 0.01) in vitro, but this effect was inhibited by treatment with rhIL-24. Conclusions: CCL2 enhances the viability of human trophoblast cell line HTR-8/SVneo cells in vitro by inhibiting the secretion of IL-24, which may be beneficial to blastocyst implantation and placental development.http://www.repdevmed.org/article.asp?issn=2096-2924;year=2017;volume=1;issue=4;spage=198;epage=203;aulast=ShaoCCL2; Cell Viability; Chorionic Trophoblasts; HTR-8/SVneo Cells; Interleukin-24 |
| spellingShingle | Jun Shao Yin-Yan He Da-Jin Li Ming-Qing Li CCL2 enhances the viability of human chorionic trophoblast cell line HTR-8/SVneo Cells by inhibiting Interleukin-24 Expression Reproductive and Developmental Medicine CCL2; Cell Viability; Chorionic Trophoblasts; HTR-8/SVneo Cells; Interleukin-24 |
| title | CCL2 enhances the viability of human chorionic trophoblast cell line HTR-8/SVneo Cells by inhibiting Interleukin-24 Expression |
| title_full | CCL2 enhances the viability of human chorionic trophoblast cell line HTR-8/SVneo Cells by inhibiting Interleukin-24 Expression |
| title_fullStr | CCL2 enhances the viability of human chorionic trophoblast cell line HTR-8/SVneo Cells by inhibiting Interleukin-24 Expression |
| title_full_unstemmed | CCL2 enhances the viability of human chorionic trophoblast cell line HTR-8/SVneo Cells by inhibiting Interleukin-24 Expression |
| title_short | CCL2 enhances the viability of human chorionic trophoblast cell line HTR-8/SVneo Cells by inhibiting Interleukin-24 Expression |
| title_sort | ccl2 enhances the viability of human chorionic trophoblast cell line htr 8 svneo cells by inhibiting interleukin 24 expression |
| topic | CCL2; Cell Viability; Chorionic Trophoblasts; HTR-8/SVneo Cells; Interleukin-24 |
| url | http://www.repdevmed.org/article.asp?issn=2096-2924;year=2017;volume=1;issue=4;spage=198;epage=203;aulast=Shao |
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