Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation
A total of twenty two germplasm lines of Vigna spp. were subjected to agroinoculation to confirm their level resistance/susceptibility against Mungbean Yellow Mosaic Virus (MYMV) using Agrobacterium construct of MYMV strain VA 239. The results showed that twelve mungbean and one urdbean germplasms...
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Format: | Article |
Language: | English |
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Indian Society of Plant Breeders
2019-06-01
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Series: | Electronic Journal of Plant Breeding |
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Online Access: | http://ejplantbreeding.org/index.php/EJPB/article/view/3162 |
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author | B. Madhumitha A. Karthikeyan M. Kanimoli Mathivathana K. Eraivan Arutkani Aiyanathan and M. Sudha |
author_facet | B. Madhumitha A. Karthikeyan M. Kanimoli Mathivathana K. Eraivan Arutkani Aiyanathan and M. Sudha |
author_sort | B. Madhumitha |
collection | DOAJ |
description | A total of twenty two germplasm lines of Vigna spp. were subjected to agroinoculation to confirm their level
resistance/susceptibility against Mungbean Yellow Mosaic Virus (MYMV) using Agrobacterium construct of MYMV strain
VA 239. The results showed that twelve mungbean and one urdbean germplasms were found to be highly susceptible (HS)
and the rest from both mungbean and urdbean showed susceptible (S) to strain VA 239. One rice bean germplasm (TNAU
Yellow) showed complete resistance. The accumulation of the viral genome in the host was confirmed by PCR amplification
by using gene-specific primer. The screening results concluded that the resistant germplasm can be utilized as a donor in
disease resistance breeding programme for generating MYMV resistant progenies in future. |
first_indexed | 2024-12-23T13:52:13Z |
format | Article |
id | doaj.art-95ac48c83e8441bfae57f5cf193eeea8 |
institution | Directory Open Access Journal |
issn | 0975-928X |
language | English |
last_indexed | 2024-12-23T13:52:13Z |
publishDate | 2019-06-01 |
publisher | Indian Society of Plant Breeders |
record_format | Article |
series | Electronic Journal of Plant Breeding |
spelling | doaj.art-95ac48c83e8441bfae57f5cf193eeea82022-12-21T17:44:34ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2019-06-0110269269810.5958/0975-928X.2019.00088.7Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculationB. MadhumithaA. KarthikeyanM. Kanimoli MathivathanaK. Eraivan Arutkani Aiyanathan andM. SudhaA total of twenty two germplasm lines of Vigna spp. were subjected to agroinoculation to confirm their level resistance/susceptibility against Mungbean Yellow Mosaic Virus (MYMV) using Agrobacterium construct of MYMV strain VA 239. The results showed that twelve mungbean and one urdbean germplasms were found to be highly susceptible (HS) and the rest from both mungbean and urdbean showed susceptible (S) to strain VA 239. One rice bean germplasm (TNAU Yellow) showed complete resistance. The accumulation of the viral genome in the host was confirmed by PCR amplification by using gene-specific primer. The screening results concluded that the resistant germplasm can be utilized as a donor in disease resistance breeding programme for generating MYMV resistant progenies in future.http://ejplantbreeding.org/index.php/EJPB/article/view/3162MungbeanUrdbeanVigna spp.GermplasmsScreeningMYMVAgroinoculationResistance |
spellingShingle | B. Madhumitha A. Karthikeyan M. Kanimoli Mathivathana K. Eraivan Arutkani Aiyanathan and M. Sudha Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation Electronic Journal of Plant Breeding Mungbean Urdbean Vigna spp. Germplasms Screening MYMV Agroinoculation Resistance |
title | Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation |
title_full | Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation |
title_fullStr | Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation |
title_full_unstemmed | Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation |
title_short | Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation |
title_sort | evaluation of vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation |
topic | Mungbean Urdbean Vigna spp. Germplasms Screening MYMV Agroinoculation Resistance |
url | http://ejplantbreeding.org/index.php/EJPB/article/view/3162 |
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