Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation

A total of twenty two germplasm lines of Vigna spp. were subjected to agroinoculation to confirm their level resistance/susceptibility against Mungbean Yellow Mosaic Virus (MYMV) using Agrobacterium construct of MYMV strain VA 239. The results showed that twelve mungbean and one urdbean germplasms...

Full description

Bibliographic Details
Main Authors: B. Madhumitha, A. Karthikeyan, M. Kanimoli Mathivathana, K. Eraivan Arutkani Aiyanathan and, M. Sudha
Format: Article
Language:English
Published: Indian Society of Plant Breeders 2019-06-01
Series:Electronic Journal of Plant Breeding
Subjects:
Online Access:http://ejplantbreeding.org/index.php/EJPB/article/view/3162
_version_ 1819239444136329216
author B. Madhumitha
A. Karthikeyan
M. Kanimoli Mathivathana
K. Eraivan Arutkani Aiyanathan and
M. Sudha
author_facet B. Madhumitha
A. Karthikeyan
M. Kanimoli Mathivathana
K. Eraivan Arutkani Aiyanathan and
M. Sudha
author_sort B. Madhumitha
collection DOAJ
description A total of twenty two germplasm lines of Vigna spp. were subjected to agroinoculation to confirm their level resistance/susceptibility against Mungbean Yellow Mosaic Virus (MYMV) using Agrobacterium construct of MYMV strain VA 239. The results showed that twelve mungbean and one urdbean germplasms were found to be highly susceptible (HS) and the rest from both mungbean and urdbean showed susceptible (S) to strain VA 239. One rice bean germplasm (TNAU Yellow) showed complete resistance. The accumulation of the viral genome in the host was confirmed by PCR amplification by using gene-specific primer. The screening results concluded that the resistant germplasm can be utilized as a donor in disease resistance breeding programme for generating MYMV resistant progenies in future.
first_indexed 2024-12-23T13:52:13Z
format Article
id doaj.art-95ac48c83e8441bfae57f5cf193eeea8
institution Directory Open Access Journal
issn 0975-928X
language English
last_indexed 2024-12-23T13:52:13Z
publishDate 2019-06-01
publisher Indian Society of Plant Breeders
record_format Article
series Electronic Journal of Plant Breeding
spelling doaj.art-95ac48c83e8441bfae57f5cf193eeea82022-12-21T17:44:34ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2019-06-0110269269810.5958/0975-928X.2019.00088.7Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculationB. MadhumithaA. KarthikeyanM. Kanimoli MathivathanaK. Eraivan Arutkani Aiyanathan andM. SudhaA total of twenty two germplasm lines of Vigna spp. were subjected to agroinoculation to confirm their level resistance/susceptibility against Mungbean Yellow Mosaic Virus (MYMV) using Agrobacterium construct of MYMV strain VA 239. The results showed that twelve mungbean and one urdbean germplasms were found to be highly susceptible (HS) and the rest from both mungbean and urdbean showed susceptible (S) to strain VA 239. One rice bean germplasm (TNAU Yellow) showed complete resistance. The accumulation of the viral genome in the host was confirmed by PCR amplification by using gene-specific primer. The screening results concluded that the resistant germplasm can be utilized as a donor in disease resistance breeding programme for generating MYMV resistant progenies in future.http://ejplantbreeding.org/index.php/EJPB/article/view/3162MungbeanUrdbeanVigna spp.GermplasmsScreeningMYMVAgroinoculationResistance
spellingShingle B. Madhumitha
A. Karthikeyan
M. Kanimoli Mathivathana
K. Eraivan Arutkani Aiyanathan and
M. Sudha
Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation
Electronic Journal of Plant Breeding
Mungbean
Urdbean
Vigna spp.
Germplasms
Screening
MYMV
Agroinoculation
Resistance
title Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation
title_full Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation
title_fullStr Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation
title_full_unstemmed Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation
title_short Evaluation of Vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation
title_sort evaluation of vigna germplasms for resistance to mungbean yellow mosaic virus using agro inoculation
topic Mungbean
Urdbean
Vigna spp.
Germplasms
Screening
MYMV
Agroinoculation
Resistance
url http://ejplantbreeding.org/index.php/EJPB/article/view/3162
work_keys_str_mv AT bmadhumitha evaluationofvignagermplasmsforresistancetomungbeanyellowmosaicvirususingagroinoculation
AT akarthikeyan evaluationofvignagermplasmsforresistancetomungbeanyellowmosaicvirususingagroinoculation
AT mkanimolimathivathana evaluationofvignagermplasmsforresistancetomungbeanyellowmosaicvirususingagroinoculation
AT keraivanarutkaniaiyanathanand evaluationofvignagermplasmsforresistancetomungbeanyellowmosaicvirususingagroinoculation
AT msudha evaluationofvignagermplasmsforresistancetomungbeanyellowmosaicvirususingagroinoculation