Sperm preservation by freeze-drying for the conservation of wild animals.
Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4 °C. Sperm are freeze-dried in a solution...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4237436?pdf=render |
| _version_ | 1811281972465500160 |
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| author | Takehito Kaneko Hideyuki Ito Hidefusa Sakamoto Manabu Onuma Miho Inoue-Murayama |
| author_facet | Takehito Kaneko Hideyuki Ito Hidefusa Sakamoto Manabu Onuma Miho Inoue-Murayama |
| author_sort | Takehito Kaneko |
| collection | DOAJ |
| description | Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4 °C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the storage and transportation of sperm. We demonstrate that chimpanzee, giraffe, jaguar, weasel and the long-haired rat sperm remain viable after freeze-drying. In all species, pronuclei were formed after the injection of freeze-dried sperm into the mouse oocytes. Although preliminary, these results may be useful for the future establishment of "freeze-drying zoo" to conserve wild animals. |
| first_indexed | 2024-04-13T01:43:19Z |
| format | Article |
| id | doaj.art-95acc2b1fdfa4836bacbc68a3a189ae5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T01:43:19Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-95acc2b1fdfa4836bacbc68a3a189ae52022-12-22T03:08:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11338110.1371/journal.pone.0113381Sperm preservation by freeze-drying for the conservation of wild animals.Takehito KanekoHideyuki ItoHidefusa SakamotoManabu OnumaMiho Inoue-MurayamaSperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4 °C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the storage and transportation of sperm. We demonstrate that chimpanzee, giraffe, jaguar, weasel and the long-haired rat sperm remain viable after freeze-drying. In all species, pronuclei were formed after the injection of freeze-dried sperm into the mouse oocytes. Although preliminary, these results may be useful for the future establishment of "freeze-drying zoo" to conserve wild animals.http://europepmc.org/articles/PMC4237436?pdf=render |
| spellingShingle | Takehito Kaneko Hideyuki Ito Hidefusa Sakamoto Manabu Onuma Miho Inoue-Murayama Sperm preservation by freeze-drying for the conservation of wild animals. PLoS ONE |
| title | Sperm preservation by freeze-drying for the conservation of wild animals. |
| title_full | Sperm preservation by freeze-drying for the conservation of wild animals. |
| title_fullStr | Sperm preservation by freeze-drying for the conservation of wild animals. |
| title_full_unstemmed | Sperm preservation by freeze-drying for the conservation of wild animals. |
| title_short | Sperm preservation by freeze-drying for the conservation of wild animals. |
| title_sort | sperm preservation by freeze drying for the conservation of wild animals |
| url | http://europepmc.org/articles/PMC4237436?pdf=render |
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