The deployment of mobile diagnostic laboratories for Ebola virus disease diagnostics in Sierra Leone and Guinea
Background: Ebola virus emerged in West Africa in December 2013. The ease of mobility, porous borders, and lack of public health infrastructure led to the largest Ebola virus disease (EVD) outbreak to date. Intervention: The 2013 EVD outbreak signalled the need for laboratory diagnostic capabilitie...
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| Format: | Article |
| Language: | English |
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AOSIS
2021-10-01
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| Series: | African Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | https://ajlmonline.org/index.php/ajlm/article/view/1414 |
| _version_ | 1828346251707416576 |
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| author | Lance D. Presser Jeanette Coffin Lamine Koivogui Allan Campbell Julian Campbell Fatmata Barrie Jone Ngobeh Zein Souma Samuel Sorie Doris Harding Alimou Camara Pepe Tohonamou Basala Traore Frank A. Hamill Joe Bogan Sharon Altmann Casey Ross Jay Mansheim Robert Hegerty Scott Poynter Scott Shearrer Carmen Asbun Brendan Karlstrand Phil Davis Jane Alam David Roberts Paul D. Stamper Jean Ndjomou Nadia Wauquier Mohamed Koroma Alhaji Munu Jason McClintock Mar Mar True Burns Stephen Krcha |
| author_facet | Lance D. Presser Jeanette Coffin Lamine Koivogui Allan Campbell Julian Campbell Fatmata Barrie Jone Ngobeh Zein Souma Samuel Sorie Doris Harding Alimou Camara Pepe Tohonamou Basala Traore Frank A. Hamill Joe Bogan Sharon Altmann Casey Ross Jay Mansheim Robert Hegerty Scott Poynter Scott Shearrer Carmen Asbun Brendan Karlstrand Phil Davis Jane Alam David Roberts Paul D. Stamper Jean Ndjomou Nadia Wauquier Mohamed Koroma Alhaji Munu Jason McClintock Mar Mar True Burns Stephen Krcha |
| author_sort | Lance D. Presser |
| collection | DOAJ |
| description | Background: Ebola virus emerged in West Africa in December 2013. The ease of mobility, porous borders, and lack of public health infrastructure led to the largest Ebola virus disease (EVD) outbreak to date.
Intervention: The 2013 EVD outbreak signalled the need for laboratory diagnostic capabilities in areas without strong public health systems. As part of the United States’ Department of Defense response, MRIGlobal was contracted to design, fabricate, equip, deploy, and operate two mobile diagnostic laboratories (MDLs). The first laboratory analysed blood samples from patients in an adjacent Ebola Treatment Centre (ETC) and buccal swabs from the deceased in the community in Moyamba, Sierra Leone. The second laboratory was deployed to support an ETC in Conakry, Guinea. The Department of Defense provided real-time quantitative reverse transcription polymerase chain reaction assays that were deployed and validated on-site.
Lessons Learnt: Prompt and accurate molecular diagnostics reduced sample turn-around times from over 24 h to under 4 h. Experienced laboratory staff tested up to 110 samples per day and on-site engineering proved necessary for MDL setup and operation. As the Ebola response slowed, the sustainment of the MDLs’ operations was prioritised, including staff training and the transition of the MDLs to local governments. Training programmes for local staff were prepared in Sierra Leone and Guinea.
Recommendations: The MRIGlobal MDL team significantly contributed to establishing increased laboratory capacity during the EVD outbreak in West Africa. Using the MDLs for molecular diagnosis is highly recommended until more sustainable solutions can be provided. |
| first_indexed | 2024-04-14T00:23:55Z |
| format | Article |
| id | doaj.art-95ad5e951b1d4b59bc6374b6ef44863d |
| institution | Directory Open Access Journal |
| issn | 2225-2002 2225-2010 |
| language | English |
| last_indexed | 2024-04-14T00:23:55Z |
| publishDate | 2021-10-01 |
| publisher | AOSIS |
| record_format | Article |
| series | African Journal of Laboratory Medicine |
| spelling | doaj.art-95ad5e951b1d4b59bc6374b6ef44863d2022-12-22T02:22:50ZengAOSISAfrican Journal of Laboratory Medicine2225-20022225-20102021-10-01101e1e610.4102/ajlm.v10i1.1414341The deployment of mobile diagnostic laboratories for Ebola virus disease diagnostics in Sierra Leone and GuineaLance D. Presser0Jeanette Coffin1Lamine Koivogui2Allan Campbell3Julian Campbell4Fatmata Barrie5Jone Ngobeh6Zein Souma7Samuel Sorie8Doris Harding9Alimou Camara10Pepe Tohonamou11Basala Traore12Frank A. Hamill13Joe Bogan14Sharon Altmann15Casey Ross16Jay Mansheim17Robert Hegerty18Scott Poynter19Scott Shearrer20Carmen Asbun21Brendan Karlstrand22Phil Davis23Jane Alam24David Roberts25Paul D. Stamper26Jean Ndjomou27Nadia Wauquier28Mohamed Koroma29Alhaji Munu30Jason McClintock31Mar Mar32True Burns33Stephen Krcha34Global Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandCentre de Recherche et de Formation en Infectiologie de Guinée, Université Gamal Abdel Nasser de Conakry, ConakryCentral Public Health Reference Laboratory, FreetownCentral Public Health Reference Laboratory, FreetownCentral Public Health Reference Laboratory, FreetownCentral Public Health Reference Laboratory, FreetownCentral Public Health Reference Laboratory, FreetownCentral Public Health Reference Laboratory, FreetownCentral Public Health Reference Laboratory, FreetownInstitut National de Santé Publique, ConakryInstitut National de Santé Publique, ConakryInstitut National de Santé Publique, ConakryGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandGlobal Engagement Program, MRIGlobal, Gaithersburg, MarylandBackground: Ebola virus emerged in West Africa in December 2013. The ease of mobility, porous borders, and lack of public health infrastructure led to the largest Ebola virus disease (EVD) outbreak to date. Intervention: The 2013 EVD outbreak signalled the need for laboratory diagnostic capabilities in areas without strong public health systems. As part of the United States’ Department of Defense response, MRIGlobal was contracted to design, fabricate, equip, deploy, and operate two mobile diagnostic laboratories (MDLs). The first laboratory analysed blood samples from patients in an adjacent Ebola Treatment Centre (ETC) and buccal swabs from the deceased in the community in Moyamba, Sierra Leone. The second laboratory was deployed to support an ETC in Conakry, Guinea. The Department of Defense provided real-time quantitative reverse transcription polymerase chain reaction assays that were deployed and validated on-site. Lessons Learnt: Prompt and accurate molecular diagnostics reduced sample turn-around times from over 24 h to under 4 h. Experienced laboratory staff tested up to 110 samples per day and on-site engineering proved necessary for MDL setup and operation. As the Ebola response slowed, the sustainment of the MDLs’ operations was prioritised, including staff training and the transition of the MDLs to local governments. Training programmes for local staff were prepared in Sierra Leone and Guinea. Recommendations: The MRIGlobal MDL team significantly contributed to establishing increased laboratory capacity during the EVD outbreak in West Africa. Using the MDLs for molecular diagnosis is highly recommended until more sustainable solutions can be provided.https://ajlmonline.org/index.php/ajlm/article/view/1414ebolaebola virussierra leoneguineadiagnosticslaboratory capacityservice expansionepidemicoutbreakoutbreak responsewest africamobile diagnostic laboratories |
| spellingShingle | Lance D. Presser Jeanette Coffin Lamine Koivogui Allan Campbell Julian Campbell Fatmata Barrie Jone Ngobeh Zein Souma Samuel Sorie Doris Harding Alimou Camara Pepe Tohonamou Basala Traore Frank A. Hamill Joe Bogan Sharon Altmann Casey Ross Jay Mansheim Robert Hegerty Scott Poynter Scott Shearrer Carmen Asbun Brendan Karlstrand Phil Davis Jane Alam David Roberts Paul D. Stamper Jean Ndjomou Nadia Wauquier Mohamed Koroma Alhaji Munu Jason McClintock Mar Mar True Burns Stephen Krcha The deployment of mobile diagnostic laboratories for Ebola virus disease diagnostics in Sierra Leone and Guinea African Journal of Laboratory Medicine ebola ebola virus sierra leone guinea diagnostics laboratory capacity service expansion epidemic outbreak outbreak response west africa mobile diagnostic laboratories |
| title | The deployment of mobile diagnostic laboratories for Ebola virus disease diagnostics in Sierra Leone and Guinea |
| title_full | The deployment of mobile diagnostic laboratories for Ebola virus disease diagnostics in Sierra Leone and Guinea |
| title_fullStr | The deployment of mobile diagnostic laboratories for Ebola virus disease diagnostics in Sierra Leone and Guinea |
| title_full_unstemmed | The deployment of mobile diagnostic laboratories for Ebola virus disease diagnostics in Sierra Leone and Guinea |
| title_short | The deployment of mobile diagnostic laboratories for Ebola virus disease diagnostics in Sierra Leone and Guinea |
| title_sort | deployment of mobile diagnostic laboratories for ebola virus disease diagnostics in sierra leone and guinea |
| topic | ebola ebola virus sierra leone guinea diagnostics laboratory capacity service expansion epidemic outbreak outbreak response west africa mobile diagnostic laboratories |
| url | https://ajlmonline.org/index.php/ajlm/article/view/1414 |
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