Quantitative In Silico Evaluation of Allergenic Proteins from <i>Anacardium occidentale</i>, <i>Carya illinoinensis</i>, <i>Juglans regia</i> and <i>Pistacia vera</i> and Their Epitopes as Precursors of Bioactive Peptides
The aim of the study presented here was to determine if there is a correlation between the presence of specific protein domains within tree nut allergens or tree nut allergen epitopes and the frequency of bioactive fragments and the predicted susceptibility to enzymatic digestion in allergenic prote...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/7/214 |
| _version_ | 1797496800916013056 |
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| author | Piotr Minkiewicz Christopher P. Mattison Małgorzata Darewicz |
| author_facet | Piotr Minkiewicz Christopher P. Mattison Małgorzata Darewicz |
| author_sort | Piotr Minkiewicz |
| collection | DOAJ |
| description | The aim of the study presented here was to determine if there is a correlation between the presence of specific protein domains within tree nut allergens or tree nut allergen epitopes and the frequency of bioactive fragments and the predicted susceptibility to enzymatic digestion in allergenic proteins from tree nuts of cashew (<i>Anacardium occidentale</i>), pecan (<i>Carya illinoinensis</i>), English walnut (<i>Juglans regia</i>) and pistachio (<i>Pistacia vera</i>) plants. These bioactive peptides are distributed along the length of the protein and are not enriched in IgE epitope sequences. Classification of proteins as bioactive peptide precursors based on the presence of specific protein domains may be a promising approach. Proteins possessing a vicilin, N-terminal family domain, or napin domain contain a relatively low occurrence of bioactive fragments. In contrast, proteins possessing the cupin 1 domain without the vicilin N-terminal family domain contain a relatively high total frequency of bioactive fragments and predicted release of bioactive fragments by the joint action of pepsin, trypsin, and chymotrypsin. This approach could be utilized in food science to simplify the selection of protein domains enriched for bioactive peptides. |
| first_indexed | 2024-03-10T03:08:45Z |
| format | Article |
| id | doaj.art-95aeae8022af4be08fcaaad86744f7aa |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T03:08:45Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-95aeae8022af4be08fcaaad86744f7aa2023-11-23T12:36:08ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-07-014473100311710.3390/cimb44070214Quantitative In Silico Evaluation of Allergenic Proteins from <i>Anacardium occidentale</i>, <i>Carya illinoinensis</i>, <i>Juglans regia</i> and <i>Pistacia vera</i> and Their Epitopes as Precursors of Bioactive PeptidesPiotr Minkiewicz0Christopher P. Mattison1Małgorzata Darewicz2Chair of Food Biochemistry, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-726 Olsztyn-Kortowo, PolandSouthern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture, New Orleans, LA 70124, USAChair of Food Biochemistry, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-726 Olsztyn-Kortowo, PolandThe aim of the study presented here was to determine if there is a correlation between the presence of specific protein domains within tree nut allergens or tree nut allergen epitopes and the frequency of bioactive fragments and the predicted susceptibility to enzymatic digestion in allergenic proteins from tree nuts of cashew (<i>Anacardium occidentale</i>), pecan (<i>Carya illinoinensis</i>), English walnut (<i>Juglans regia</i>) and pistachio (<i>Pistacia vera</i>) plants. These bioactive peptides are distributed along the length of the protein and are not enriched in IgE epitope sequences. Classification of proteins as bioactive peptide precursors based on the presence of specific protein domains may be a promising approach. Proteins possessing a vicilin, N-terminal family domain, or napin domain contain a relatively low occurrence of bioactive fragments. In contrast, proteins possessing the cupin 1 domain without the vicilin N-terminal family domain contain a relatively high total frequency of bioactive fragments and predicted release of bioactive fragments by the joint action of pepsin, trypsin, and chymotrypsin. This approach could be utilized in food science to simplify the selection of protein domains enriched for bioactive peptides.https://www.mdpi.com/1467-3045/44/7/214food allergytree nutbioactivepeptidebioinformaticscupin |
| spellingShingle | Piotr Minkiewicz Christopher P. Mattison Małgorzata Darewicz Quantitative In Silico Evaluation of Allergenic Proteins from <i>Anacardium occidentale</i>, <i>Carya illinoinensis</i>, <i>Juglans regia</i> and <i>Pistacia vera</i> and Their Epitopes as Precursors of Bioactive Peptides Current Issues in Molecular Biology food allergy tree nut bioactive peptide bioinformatics cupin |
| title | Quantitative In Silico Evaluation of Allergenic Proteins from <i>Anacardium occidentale</i>, <i>Carya illinoinensis</i>, <i>Juglans regia</i> and <i>Pistacia vera</i> and Their Epitopes as Precursors of Bioactive Peptides |
| title_full | Quantitative In Silico Evaluation of Allergenic Proteins from <i>Anacardium occidentale</i>, <i>Carya illinoinensis</i>, <i>Juglans regia</i> and <i>Pistacia vera</i> and Their Epitopes as Precursors of Bioactive Peptides |
| title_fullStr | Quantitative In Silico Evaluation of Allergenic Proteins from <i>Anacardium occidentale</i>, <i>Carya illinoinensis</i>, <i>Juglans regia</i> and <i>Pistacia vera</i> and Their Epitopes as Precursors of Bioactive Peptides |
| title_full_unstemmed | Quantitative In Silico Evaluation of Allergenic Proteins from <i>Anacardium occidentale</i>, <i>Carya illinoinensis</i>, <i>Juglans regia</i> and <i>Pistacia vera</i> and Their Epitopes as Precursors of Bioactive Peptides |
| title_short | Quantitative In Silico Evaluation of Allergenic Proteins from <i>Anacardium occidentale</i>, <i>Carya illinoinensis</i>, <i>Juglans regia</i> and <i>Pistacia vera</i> and Their Epitopes as Precursors of Bioactive Peptides |
| title_sort | quantitative in silico evaluation of allergenic proteins from i anacardium occidentale i i carya illinoinensis i i juglans regia i and i pistacia vera i and their epitopes as precursors of bioactive peptides |
| topic | food allergy tree nut bioactive peptide bioinformatics cupin |
| url | https://www.mdpi.com/1467-3045/44/7/214 |
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