Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9
ASGR1 is a liver-specific surface marker that has been used to purify human pluripotent stem cell (PSC)-derived hepatocytes (iHeps). Furthermore, ASGR1+ iHeps represents a more mature subpopulation of iHeps. To utilize this marker for optimizing iHep differentiation and purification, we substituted...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-07-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122001490 |
| _version_ | 1811242298184302592 |
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| author | Jian Fu Lixiang Jiang Baorong Yu Yuqing Liu Rui Wei Yang Hu Wai-In Ho Bo Yang Meng Chu Hung-Fat Tse Jiayin Yang |
| author_facet | Jian Fu Lixiang Jiang Baorong Yu Yuqing Liu Rui Wei Yang Hu Wai-In Ho Bo Yang Meng Chu Hung-Fat Tse Jiayin Yang |
| author_sort | Jian Fu |
| collection | DOAJ |
| description | ASGR1 is a liver-specific surface marker that has been used to purify human pluripotent stem cell (PSC)-derived hepatocytes (iHeps). Furthermore, ASGR1+ iHeps represents a more mature subpopulation of iHeps. To utilize this marker for optimizing iHep differentiation and purification, we substituted the stop coden of ASGR1 with a fluorescent reporter protein mCherry in a human iPSC line iPSN0052 via CRISPR/Cas9-mediated homologus recombination. The generated CIBi010-A enableds us to monitor ASGR1 expression during hepatic differentiation and thus can be used to optimize our hepatic differentiation procedures. |
| first_indexed | 2024-04-12T13:49:39Z |
| format | Article |
| id | doaj.art-95af9e66ea7c478193f1276176135d6e |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-12T13:49:39Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-95af9e66ea7c478193f1276176135d6e2022-12-22T03:30:34ZengElsevierStem Cell Research1873-50612022-07-0162102800Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9Jian Fu0Lixiang Jiang1Baorong Yu2Yuqing Liu3Rui Wei4Yang Hu5Wai-In Ho6Bo Yang7Meng Chu8Hung-Fat Tse9Jiayin Yang10Cell Inspire Biotechnology Co., Ltd., Shenzhen 518101, ChinaCell Inspire Biotechnology Co., Ltd., Shenzhen 518101, ChinaCell Inspire Biotechnology Co., Ltd., Shenzhen 518101, ChinaCell Inspire Biotechnology Co., Ltd., Shenzhen 518101, ChinaCardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, ChinaCardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, ChinaCardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, ChinaCell Inspire Biotechnology Co., Ltd., Shenzhen 518101, ChinaCell Inspire Biotechnology Co., Ltd., Shenzhen 518101, ChinaCardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China; Cardiac and Vascular Center, Hong Kong University Shenzhen Hospital, Shenzhen 518053, China; Hong Kong-Guangdong Joint Laboratory on Stem Cell and Regenerative Medicine, the University of Hong Kong, Hong Kong, China; Center for Translational Stem Cell Biology, Hong Kong SAR, China; Corresponding authors at: Cell Inspire Therapeutics Co., Ltd., Shenzhen 518101, China (J. Yang). Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China (H.-F. Tse).Cell Inspire Therapeutics Co., Ltd., Shenzhen 518101, China; Corresponding authors at: Cell Inspire Therapeutics Co., Ltd., Shenzhen 518101, China (J. Yang). Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China (H.-F. Tse).ASGR1 is a liver-specific surface marker that has been used to purify human pluripotent stem cell (PSC)-derived hepatocytes (iHeps). Furthermore, ASGR1+ iHeps represents a more mature subpopulation of iHeps. To utilize this marker for optimizing iHep differentiation and purification, we substituted the stop coden of ASGR1 with a fluorescent reporter protein mCherry in a human iPSC line iPSN0052 via CRISPR/Cas9-mediated homologus recombination. The generated CIBi010-A enableds us to monitor ASGR1 expression during hepatic differentiation and thus can be used to optimize our hepatic differentiation procedures.http://www.sciencedirect.com/science/article/pii/S1873506122001490 |
| spellingShingle | Jian Fu Lixiang Jiang Baorong Yu Yuqing Liu Rui Wei Yang Hu Wai-In Ho Bo Yang Meng Chu Hung-Fat Tse Jiayin Yang Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9 Stem Cell Research |
| title | Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9 |
| title_full | Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9 |
| title_fullStr | Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9 |
| title_full_unstemmed | Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9 |
| title_short | Generation of a human iPSC line CIBi010-A with a reporter for ASGR1 using CRISPR/Cas9 |
| title_sort | generation of a human ipsc line cibi010 a with a reporter for asgr1 using crispr cas9 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122001490 |
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