Discovery of induced point mutations in maize genes by TILLING
<p>Abstract</p> <p>Background</p> <p>Going from a gene sequence to its function in the context of a whole organism requires a strategy for targeting mutations, referred to as reverse genetics. Reverse genetics is highly desirable in the modern genomics era; however, the...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-07-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/4/12 |
| _version_ | 1819088013721862144 |
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| author | Enns Linda C Codomo Christine A Bowers Elisabeth Young Kim Burtner Chris Springer Nathan Weil Clifford Reynolds Steven H Till Bradley J Odden Anthony R Greene Elizabeth A Comai Luca Henikoff Steven |
| author_facet | Enns Linda C Codomo Christine A Bowers Elisabeth Young Kim Burtner Chris Springer Nathan Weil Clifford Reynolds Steven H Till Bradley J Odden Anthony R Greene Elizabeth A Comai Luca Henikoff Steven |
| author_sort | Enns Linda C |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Going from a gene sequence to its function in the context of a whole organism requires a strategy for targeting mutations, referred to as reverse genetics. Reverse genetics is highly desirable in the modern genomics era; however, the most powerful methods are generally restricted to a few model organisms. Previously, we introduced a reverse-genetic strategy with the potential for general applicability to organisms that lack well-developed genetic tools. Our TILLING (Targeting Induced Local Lesions IN Genomes) method uses chemical mutagenesis followed by screening for single-base changes to discover induced mutations that alter protein function. TILLING was shown to be an effective reverse genetic strategy by the establishment of a high-throughput TILLING facility and the delivery of thousands of point mutations in hundreds of Arabidopsis genes to members of the plant biology community.</p> <p>Results</p> <p>We demonstrate that high-throughput TILLING is applicable to maize, an important crop plant with a large genome but with limited reverse-genetic resources currently available. We screened pools of DNA samples for mutations in 1-kb segments from 11 different genes, obtaining 17 independent induced mutations from a population of 750 pollen-mutagenized maize plants. One of the genes targeted was the DMT102 chromomethylase gene, for which we obtained an allelic series of three missense mutations that are predicted to be strongly deleterious.</p> <p>Conclusions</p> <p>Our findings indicate that TILLING is a broadly applicable and efficient reverse-genetic strategy. We are establishing a public TILLING service for maize modeled on the existing Arabidopsis TILLING Project.</p> |
| first_indexed | 2024-12-21T21:45:17Z |
| format | Article |
| id | doaj.art-95afd8aac73046cc92045c0d26f81a50 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T21:45:17Z |
| publishDate | 2004-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-95afd8aac73046cc92045c0d26f81a502022-12-21T18:49:15ZengBMCBMC Plant Biology1471-22292004-07-01411210.1186/1471-2229-4-12Discovery of induced point mutations in maize genes by TILLINGEnns Linda CCodomo Christine ABowers ElisabethYoung KimBurtner ChrisSpringer NathanWeil CliffordReynolds Steven HTill Bradley JOdden Anthony RGreene Elizabeth AComai LucaHenikoff Steven<p>Abstract</p> <p>Background</p> <p>Going from a gene sequence to its function in the context of a whole organism requires a strategy for targeting mutations, referred to as reverse genetics. Reverse genetics is highly desirable in the modern genomics era; however, the most powerful methods are generally restricted to a few model organisms. Previously, we introduced a reverse-genetic strategy with the potential for general applicability to organisms that lack well-developed genetic tools. Our TILLING (Targeting Induced Local Lesions IN Genomes) method uses chemical mutagenesis followed by screening for single-base changes to discover induced mutations that alter protein function. TILLING was shown to be an effective reverse genetic strategy by the establishment of a high-throughput TILLING facility and the delivery of thousands of point mutations in hundreds of Arabidopsis genes to members of the plant biology community.</p> <p>Results</p> <p>We demonstrate that high-throughput TILLING is applicable to maize, an important crop plant with a large genome but with limited reverse-genetic resources currently available. We screened pools of DNA samples for mutations in 1-kb segments from 11 different genes, obtaining 17 independent induced mutations from a population of 750 pollen-mutagenized maize plants. One of the genes targeted was the DMT102 chromomethylase gene, for which we obtained an allelic series of three missense mutations that are predicted to be strongly deleterious.</p> <p>Conclusions</p> <p>Our findings indicate that TILLING is a broadly applicable and efficient reverse-genetic strategy. We are establishing a public TILLING service for maize modeled on the existing Arabidopsis TILLING Project.</p>http://www.biomedcentral.com/1471-2229/4/12 |
| spellingShingle | Enns Linda C Codomo Christine A Bowers Elisabeth Young Kim Burtner Chris Springer Nathan Weil Clifford Reynolds Steven H Till Bradley J Odden Anthony R Greene Elizabeth A Comai Luca Henikoff Steven Discovery of induced point mutations in maize genes by TILLING BMC Plant Biology |
| title | Discovery of induced point mutations in maize genes by TILLING |
| title_full | Discovery of induced point mutations in maize genes by TILLING |
| title_fullStr | Discovery of induced point mutations in maize genes by TILLING |
| title_full_unstemmed | Discovery of induced point mutations in maize genes by TILLING |
| title_short | Discovery of induced point mutations in maize genes by TILLING |
| title_sort | discovery of induced point mutations in maize genes by tilling |
| url | http://www.biomedcentral.com/1471-2229/4/12 |
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