Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus
Microspore embryogenesis is a model system of plant cell reprogramming, totipotency acquisition, stress response and embryogenesis initiation. This in vitro system constitutes an important biotechnological tool for haploid and doubled-haploid plant production, very useful for crop breeding. In this...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2019-07-01
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| Series: | The EuroBiotech Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/ebtj-2019-0018 |
| _version_ | 1831763788484837376 |
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| author | Berenguer Eduardo Solís María-Teresa Pérez-Pérez Yolanda Testillano Pilar S. |
| author_facet | Berenguer Eduardo Solís María-Teresa Pérez-Pérez Yolanda Testillano Pilar S. |
| author_sort | Berenguer Eduardo |
| collection | DOAJ |
| description | Microspore embryogenesis is a model system of plant cell reprogramming, totipotency acquisition, stress response and embryogenesis initiation. This in vitro system constitutes an important biotechnological tool for haploid and doubled-haploid plant production, very useful for crop breeding. In this process, microspores (cells that produce pollen grains in planta) are reprogrammed toward embryogenesis by specific stress treatment, but many microspores die after the stress. The occurrence of cell death is a serious limiting problem that greatly reduces microspore embryogenesis yield. In animals, increasing evidence has revealed caspase proteolytic activities as essential executioners of programmed cell death (PCD) processes, however, less is known in plants. Although plant genomes do not contain caspase homologues, caspase-like proteolytic activities have been detected in many plant PCD processes. In the present study, we have analysed caspase 3-like activity and its involvement in stress-induced cell death during initial stages of microspore embryogenesis of Brassica napus. After stress treatment to induce embryogenesis, isolated microspore cultures showed high levels of cell death and caspase 3-like proteolytic activity was induced. Treatments with specific inhibitor of caspase 3-like activity reduced cell death and increased embryogenesis induction efficiency. Our findings indicate the involvement of proteases with caspase 3-like activity in the initiation and/or execution of cell death at early microspore embryogenesis in B. napus, giving new insights into the pathways of stress-induced cell death in plants and opening a new way to improve in vitro embryogenesis efficiency by using chemical modulators of cell death proteases. |
| first_indexed | 2024-12-22T05:32:47Z |
| format | Article |
| id | doaj.art-95aff62ee4314435be7e454261d7ebec |
| institution | Directory Open Access Journal |
| issn | 2564-615X |
| language | English |
| last_indexed | 2024-12-22T05:32:47Z |
| publishDate | 2019-07-01 |
| publisher | Sciendo |
| record_format | Article |
| series | The EuroBiotech Journal |
| spelling | doaj.art-95aff62ee4314435be7e454261d7ebec2022-12-21T18:37:25ZengSciendoThe EuroBiotech Journal2564-615X2019-07-013315215910.2478/ebtj-2019-0018ebtj-2019-0018Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napusBerenguer Eduardo0Solís María-Teresa1Pérez-Pérez Yolanda2Testillano Pilar S.3Pollen Biotechnology of Crop Plants laboratory, Biological Research Center, CIBCSIC, Madrid, SpainPollen Biotechnology of Crop Plants laboratory, Biological Research Center, CIBCSIC, Madrid, SpainPollen Biotechnology of Crop Plants laboratory, Biological Research Center, CIBCSIC, Madrid, SpainPollen Biotechnology of Crop Plants laboratory, Biological Research Center, CIBCSIC, Madrid, SpainMicrospore embryogenesis is a model system of plant cell reprogramming, totipotency acquisition, stress response and embryogenesis initiation. This in vitro system constitutes an important biotechnological tool for haploid and doubled-haploid plant production, very useful for crop breeding. In this process, microspores (cells that produce pollen grains in planta) are reprogrammed toward embryogenesis by specific stress treatment, but many microspores die after the stress. The occurrence of cell death is a serious limiting problem that greatly reduces microspore embryogenesis yield. In animals, increasing evidence has revealed caspase proteolytic activities as essential executioners of programmed cell death (PCD) processes, however, less is known in plants. Although plant genomes do not contain caspase homologues, caspase-like proteolytic activities have been detected in many plant PCD processes. In the present study, we have analysed caspase 3-like activity and its involvement in stress-induced cell death during initial stages of microspore embryogenesis of Brassica napus. After stress treatment to induce embryogenesis, isolated microspore cultures showed high levels of cell death and caspase 3-like proteolytic activity was induced. Treatments with specific inhibitor of caspase 3-like activity reduced cell death and increased embryogenesis induction efficiency. Our findings indicate the involvement of proteases with caspase 3-like activity in the initiation and/or execution of cell death at early microspore embryogenesis in B. napus, giving new insights into the pathways of stress-induced cell death in plants and opening a new way to improve in vitro embryogenesis efficiency by using chemical modulators of cell death proteases.https://doi.org/10.2478/ebtj-2019-0018cell deathcaspase 3-like activitymicrospore embryogenesisstress treatment |
| spellingShingle | Berenguer Eduardo Solís María-Teresa Pérez-Pérez Yolanda Testillano Pilar S. Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus The EuroBiotech Journal cell death caspase 3-like activity microspore embryogenesis stress treatment |
| title | Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus |
| title_full | Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus |
| title_fullStr | Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus |
| title_full_unstemmed | Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus |
| title_short | Proteases with caspase 3-like activity participate in cell death during stress-induced microspore embryogenesis of Brassica napus |
| title_sort | proteases with caspase 3 like activity participate in cell death during stress induced microspore embryogenesis of brassica napus |
| topic | cell death caspase 3-like activity microspore embryogenesis stress treatment |
| url | https://doi.org/10.2478/ebtj-2019-0018 |
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