CD83 expression regulates antibody production in response to influenza A virus infection
Abstract Background CD83 is known to regulate lymphocyte maturation, activation, homeostasis, and antibody response to immunization and infection. While CD83 has a major part in B cell function, its role in influenza A virus infection has not yet been investigated. Methods We investigated the role o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-12-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-020-01465-0 |
| _version_ | 1818392354808135680 |
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| author | Madhav Akauliya Avishekh Gautam Sony Maharjan Byoung Kwon Park Jinsoo Kim Hyung-Joo Kwon |
| author_facet | Madhav Akauliya Avishekh Gautam Sony Maharjan Byoung Kwon Park Jinsoo Kim Hyung-Joo Kwon |
| author_sort | Madhav Akauliya |
| collection | DOAJ |
| description | Abstract Background CD83 is known to regulate lymphocyte maturation, activation, homeostasis, and antibody response to immunization and infection. While CD83 has a major part in B cell function, its role in influenza A virus infection has not yet been investigated. Methods We investigated the role of CD83 using C57BL/6J wild type mice and CD83 knockout (KO) mice after intraperitoneal administration of the influenza A/WSN/1933 virus. We analyzed cells of the peritoneal cavity, splenocytes, and cells of the bone marrow with FACS to investigate CD83 expression and cell population change in response to the virus infection. ELISA was performed with sera and peritoneal cavity fluids to detect A/WSN/1933 virus-specific IgG and the subclasses of IgG. Results FACS analysis data showed a transient but distinct induction of CD83 expression in the peritoneal B cells of wild type mice. CD83 KO mice exhibited a delayed recovery of B cells in the bone marrow after influenza virus infection and overall, a smaller T cell population compared to wild type mice. The peritoneal cavity and serum of the wild type mice contained a high titer of IgG within 14 days after infection, whereas the CD83 KO mice had a very low titer of IgG. Conclusions These results show the importance of CD83 in lymphocytes homeostasis and antibody production during influenza A virus infection. |
| first_indexed | 2024-12-14T05:28:05Z |
| format | Article |
| id | doaj.art-95bb19ea5ef5437b9a1c78a5ff84c536 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-14T05:28:05Z |
| publishDate | 2020-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-95bb19ea5ef5437b9a1c78a5ff84c5362022-12-21T23:15:28ZengBMCVirology Journal1743-422X2020-12-0117111110.1186/s12985-020-01465-0CD83 expression regulates antibody production in response to influenza A virus infectionMadhav Akauliya0Avishekh Gautam1Sony Maharjan2Byoung Kwon Park3Jinsoo Kim4Hyung-Joo Kwon5Department of Microbiology, College of Medicine, Hallym UniversityDepartment of Microbiology, College of Medicine, Hallym UniversityInstitute of Medical Science, College of Medicine, Hallym UniversityInstitute of Medical Science, College of Medicine, Hallym UniversityDepartment of Microbiology, College of Medicine, Hallym UniversityDepartment of Microbiology, College of Medicine, Hallym UniversityAbstract Background CD83 is known to regulate lymphocyte maturation, activation, homeostasis, and antibody response to immunization and infection. While CD83 has a major part in B cell function, its role in influenza A virus infection has not yet been investigated. Methods We investigated the role of CD83 using C57BL/6J wild type mice and CD83 knockout (KO) mice after intraperitoneal administration of the influenza A/WSN/1933 virus. We analyzed cells of the peritoneal cavity, splenocytes, and cells of the bone marrow with FACS to investigate CD83 expression and cell population change in response to the virus infection. ELISA was performed with sera and peritoneal cavity fluids to detect A/WSN/1933 virus-specific IgG and the subclasses of IgG. Results FACS analysis data showed a transient but distinct induction of CD83 expression in the peritoneal B cells of wild type mice. CD83 KO mice exhibited a delayed recovery of B cells in the bone marrow after influenza virus infection and overall, a smaller T cell population compared to wild type mice. The peritoneal cavity and serum of the wild type mice contained a high titer of IgG within 14 days after infection, whereas the CD83 KO mice had a very low titer of IgG. Conclusions These results show the importance of CD83 in lymphocytes homeostasis and antibody production during influenza A virus infection.https://doi.org/10.1186/s12985-020-01465-0Antibody productionB cellsCD83Influenza A virusPeritoneal cavity |
| spellingShingle | Madhav Akauliya Avishekh Gautam Sony Maharjan Byoung Kwon Park Jinsoo Kim Hyung-Joo Kwon CD83 expression regulates antibody production in response to influenza A virus infection Virology Journal Antibody production B cells CD83 Influenza A virus Peritoneal cavity |
| title | CD83 expression regulates antibody production in response to influenza A virus infection |
| title_full | CD83 expression regulates antibody production in response to influenza A virus infection |
| title_fullStr | CD83 expression regulates antibody production in response to influenza A virus infection |
| title_full_unstemmed | CD83 expression regulates antibody production in response to influenza A virus infection |
| title_short | CD83 expression regulates antibody production in response to influenza A virus infection |
| title_sort | cd83 expression regulates antibody production in response to influenza a virus infection |
| topic | Antibody production B cells CD83 Influenza A virus Peritoneal cavity |
| url | https://doi.org/10.1186/s12985-020-01465-0 |
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