Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture
<p>Abstract</p> <p>Background</p> <p>Poly(amidoamine)s (PAAs) are synthetic polymers endowed with many biologically interesting properties, being highly biocompatible, non toxic and biodegradable. Hydrogels based on PAAs can be easily modified during the synthesis by th...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-11-01
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| Series: | Journal of Nanobiotechnology |
| Online Access: | http://www.jnanobiotechnology.com/content/6/1/14 |
| _version_ | 1817993273612959744 |
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| author | Lenardi Cristina Gianfelice Antonella Indrieri Marco Rodighiero Simona Emilitri Elisa Jacchetti Emanuela Podestà Alessandro Ranucci Elisabetta Ferruti Paolo Milani Paolo |
| author_facet | Lenardi Cristina Gianfelice Antonella Indrieri Marco Rodighiero Simona Emilitri Elisa Jacchetti Emanuela Podestà Alessandro Ranucci Elisabetta Ferruti Paolo Milani Paolo |
| author_sort | Lenardi Cristina |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Poly(amidoamine)s (PAAs) are synthetic polymers endowed with many biologically interesting properties, being highly biocompatible, non toxic and biodegradable. Hydrogels based on PAAs can be easily modified during the synthesis by the introduction of functional co-monomers. Aim of this work is the development and testing of novel amphoteric nanosized poly(amidoamine) hydrogel film incorporating 4-aminobutylguanidine (agmatine) moieties to create RGD-mimicking repeating units for promoting cell adhesion.</p> <p>Results</p> <p>A systematic comparative study of the response of an epithelial cell line was performed on hydrogels with agmatine and on non-functionalized amphoteric poly(amidoamine) hydrogels and tissue culture plastic substrates. The cell adhesion on the agmatine containing substrates was comparable to that on plastic substrates and significantly enhanced with respect to the non-functionalized controls. Interestingly, spreading and proliferation on the functionalized supports are slower than on plastic exhibiting the possibility of an easier control of the cell growth kinetics. In order to favor the handling of the samples, a procedure for the production of bi-layered constructs was also developed by means the deposition via spin coating of a thin layer of hydrogel on a pre-treated cover slip.</p> <p>Conclusion</p> <p>The obtained results reveal that PAAs hydrogels can be profitably functionalized and, in general, undergo physical and chemical modifications to meet specific requirements. In particular the incorporation of agmatine warrants good potential in the field of cell culturing and the development of supported functionalized hydrogels on cover glass are very promising substrates for applications in cell screening devices.</p> |
| first_indexed | 2024-04-14T01:36:43Z |
| format | Article |
| id | doaj.art-95bbf76c35ee423385e04256cdf28950 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-14T01:36:43Z |
| publishDate | 2008-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-95bbf76c35ee423385e04256cdf289502022-12-22T02:19:54ZengBMCJournal of Nanobiotechnology1477-31552008-11-01611410.1186/1477-3155-6-14Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell cultureLenardi CristinaGianfelice AntonellaIndrieri MarcoRodighiero SimonaEmilitri ElisaJacchetti EmanuelaPodestà AlessandroRanucci ElisabettaFerruti PaoloMilani Paolo<p>Abstract</p> <p>Background</p> <p>Poly(amidoamine)s (PAAs) are synthetic polymers endowed with many biologically interesting properties, being highly biocompatible, non toxic and biodegradable. Hydrogels based on PAAs can be easily modified during the synthesis by the introduction of functional co-monomers. Aim of this work is the development and testing of novel amphoteric nanosized poly(amidoamine) hydrogel film incorporating 4-aminobutylguanidine (agmatine) moieties to create RGD-mimicking repeating units for promoting cell adhesion.</p> <p>Results</p> <p>A systematic comparative study of the response of an epithelial cell line was performed on hydrogels with agmatine and on non-functionalized amphoteric poly(amidoamine) hydrogels and tissue culture plastic substrates. The cell adhesion on the agmatine containing substrates was comparable to that on plastic substrates and significantly enhanced with respect to the non-functionalized controls. Interestingly, spreading and proliferation on the functionalized supports are slower than on plastic exhibiting the possibility of an easier control of the cell growth kinetics. In order to favor the handling of the samples, a procedure for the production of bi-layered constructs was also developed by means the deposition via spin coating of a thin layer of hydrogel on a pre-treated cover slip.</p> <p>Conclusion</p> <p>The obtained results reveal that PAAs hydrogels can be profitably functionalized and, in general, undergo physical and chemical modifications to meet specific requirements. In particular the incorporation of agmatine warrants good potential in the field of cell culturing and the development of supported functionalized hydrogels on cover glass are very promising substrates for applications in cell screening devices.</p>http://www.jnanobiotechnology.com/content/6/1/14 |
| spellingShingle | Lenardi Cristina Gianfelice Antonella Indrieri Marco Rodighiero Simona Emilitri Elisa Jacchetti Emanuela Podestà Alessandro Ranucci Elisabetta Ferruti Paolo Milani Paolo Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture Journal of Nanobiotechnology |
| title | Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture |
| title_full | Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture |
| title_fullStr | Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture |
| title_full_unstemmed | Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture |
| title_short | Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture |
| title_sort | biomimetic poly amidoamine hydrogels as synthetic materials for cell culture |
| url | http://www.jnanobiotechnology.com/content/6/1/14 |
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