Genetic characterization of four strains porcine circovirus-like viruses in pigs with diarrhea in Hunan Province of China
In this study, we detected a circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus [named Po-Circo-like (PCL) virus] in intestinal tissue and fecal samples of pigs. PCL virus contains a single-stranded DNA genome, and ORF1 encodes the Rep and not the typical capsid...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1126707/full |
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| author | Chihai Ji Chihai Ji Chihai Ji Meng Zeng Meng Zeng Meng Zeng Yingfang Wei Xiaocheng Lv Xiaocheng Lv Xiaocheng Lv Yuan Sun Yuan Sun Yuan Sun Jingyun Ma Jingyun Ma Jingyun Ma |
| author_facet | Chihai Ji Chihai Ji Chihai Ji Meng Zeng Meng Zeng Meng Zeng Yingfang Wei Xiaocheng Lv Xiaocheng Lv Xiaocheng Lv Yuan Sun Yuan Sun Yuan Sun Jingyun Ma Jingyun Ma Jingyun Ma |
| author_sort | Chihai Ji |
| collection | DOAJ |
| description | In this study, we detected a circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus [named Po-Circo-like (PCL) virus] in intestinal tissue and fecal samples of pigs. PCL virus contains a single-stranded DNA genome, and ORF1 encodes the Rep and not the typical capsid protein encoded in PCV. The Rep protein may be responsible for viral genome replication. In addition, PCL virus may be one of the pathogens causing diarrhea symptoms in pigs. We identified four strains of PCL virus in two different pig farms with severe diarrhea outbreaks in Hunan Province, China. The strains in this study share 85.7–99.7% nucleic acid identity and 84.7–100% amino acid identity with Rep of the reference strains. A multiple sequence alignment of these PCL viruses and Bo-Circo-like CH showed a identity of 93.2% for the Rep protein, and the nucleotide identity was 86.7–89.3%. Moreover, Bo-Circo-like CH and HN75, HN39-01, HN39-02 had similar stem-loop sequences. In conclusion, the present study is the first detailed report of the PCL virus in Hunan provinces, which is a potential new virus in pigs that might be involved in cross-species transmission. Further investigation is needed to determine the pathogenesis of this virus and its epidemiologic impact. |
| first_indexed | 2024-04-10T06:32:05Z |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T06:32:05Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-95be6da864aa4cfea3da9e5dd8d67c092023-03-01T07:01:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11267071126707Genetic characterization of four strains porcine circovirus-like viruses in pigs with diarrhea in Hunan Province of ChinaChihai Ji0Chihai Ji1Chihai Ji2Meng Zeng3Meng Zeng4Meng Zeng5Yingfang Wei6Xiaocheng Lv7Xiaocheng Lv8Xiaocheng Lv9Yuan Sun10Yuan Sun11Yuan Sun12Jingyun Ma13Jingyun Ma14Jingyun Ma15Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, ChinaKey Laboratory of Animal Health Aquaculture and Environmental Control, College of Animal Science, South China Agricultural University, Guangzhou, ChinaIn this study, we detected a circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA virus [named Po-Circo-like (PCL) virus] in intestinal tissue and fecal samples of pigs. PCL virus contains a single-stranded DNA genome, and ORF1 encodes the Rep and not the typical capsid protein encoded in PCV. The Rep protein may be responsible for viral genome replication. In addition, PCL virus may be one of the pathogens causing diarrhea symptoms in pigs. We identified four strains of PCL virus in two different pig farms with severe diarrhea outbreaks in Hunan Province, China. The strains in this study share 85.7–99.7% nucleic acid identity and 84.7–100% amino acid identity with Rep of the reference strains. A multiple sequence alignment of these PCL viruses and Bo-Circo-like CH showed a identity of 93.2% for the Rep protein, and the nucleotide identity was 86.7–89.3%. Moreover, Bo-Circo-like CH and HN75, HN39-01, HN39-02 had similar stem-loop sequences. In conclusion, the present study is the first detailed report of the PCL virus in Hunan provinces, which is a potential new virus in pigs that might be involved in cross-species transmission. Further investigation is needed to determine the pathogenesis of this virus and its epidemiologic impact.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1126707/fullCRESS DNA virusPo-Circo-like virusRep proteinphylogenetic analysisepidemiologic |
| spellingShingle | Chihai Ji Chihai Ji Chihai Ji Meng Zeng Meng Zeng Meng Zeng Yingfang Wei Xiaocheng Lv Xiaocheng Lv Xiaocheng Lv Yuan Sun Yuan Sun Yuan Sun Jingyun Ma Jingyun Ma Jingyun Ma Genetic characterization of four strains porcine circovirus-like viruses in pigs with diarrhea in Hunan Province of China Frontiers in Microbiology CRESS DNA virus Po-Circo-like virus Rep protein phylogenetic analysis epidemiologic |
| title | Genetic characterization of four strains porcine circovirus-like viruses in pigs with diarrhea in Hunan Province of China |
| title_full | Genetic characterization of four strains porcine circovirus-like viruses in pigs with diarrhea in Hunan Province of China |
| title_fullStr | Genetic characterization of four strains porcine circovirus-like viruses in pigs with diarrhea in Hunan Province of China |
| title_full_unstemmed | Genetic characterization of four strains porcine circovirus-like viruses in pigs with diarrhea in Hunan Province of China |
| title_short | Genetic characterization of four strains porcine circovirus-like viruses in pigs with diarrhea in Hunan Province of China |
| title_sort | genetic characterization of four strains porcine circovirus like viruses in pigs with diarrhea in hunan province of china |
| topic | CRESS DNA virus Po-Circo-like virus Rep protein phylogenetic analysis epidemiologic |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1126707/full |
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