Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production
Varalu is an early maturing rice variety widely grown in the rainfed ecosystem preferred for its grain type and cooking quality. However, the yield of Varalu is substantially low since it is being affected by reproductive drought stress along with the blast disease. The genetic improvement of Varalu...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Rice Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630821000688 |
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| author | Vishalakshi Balija Umakanth Bangale Senguttuvel Ponnuvel Kalyani Makarand Barbadikar Srinivas Prasad Madamshetty Sanjeeva Rao Durbha Hari Yadla Sheshu Madhav Maganti |
| author_facet | Vishalakshi Balija Umakanth Bangale Senguttuvel Ponnuvel Kalyani Makarand Barbadikar Srinivas Prasad Madamshetty Sanjeeva Rao Durbha Hari Yadla Sheshu Madhav Maganti |
| author_sort | Vishalakshi Balija |
| collection | DOAJ |
| description | Varalu is an early maturing rice variety widely grown in the rainfed ecosystem preferred for its grain type and cooking quality. However, the yield of Varalu is substantially low since it is being affected by reproductive drought stress along with the blast disease. The genetic improvement of Varalu was done by introgressing a major yield QTL, qDTY12.1, along with two major blast resistance genes i.e. Pi54 and Pi1 through marker-assisted backcross breeding. Both traits were transferred till BC2 generation and intercrossing was followed to pyramid the two traits. Stringent foreground selection was carried out using linked markers as well as peak markers (RM28099, RM28130, RM511 and RM28163) for the targeted QTL (qDTY12.1), RM206 for Pi54 and RM224 for Pi1. Extensive background selection was done using genome-wide SSR markers. Six best lines (MSM-36, MSM-49, MSM-53, MSM-57, MSM-60 and MSM-63) having qDTY12.1 and two blast resistance genes in homozygous condition with recurrent parent genome of 95.0%-96.5% having minimal linkage drag of about 0.1 to 0.7 Mb were identified. These lines showed yield advantage under drought stress as well as irrigated conditions. MSM-36 showed better performance in the national coordinated trials conducted across India, which indicated that improved lines of Varalu expected to replace Varalu and may have an important role in sustaining rice production. The present study demonstrated the successful marker-assisted pyramiding strategy for introgression of genes/QTLs conferring biotic stress resistance and yield under abiotic stress in rice. |
| first_indexed | 2024-12-21T16:49:26Z |
| format | Article |
| id | doaj.art-95bf38f71dd74d34b66415b12d930b09 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-12-21T16:49:26Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj.art-95bf38f71dd74d34b66415b12d930b092022-12-21T18:56:55ZengElsevierRice Science1672-63082021-09-01285493500Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice ProductionVishalakshi Balija0Umakanth Bangale1Senguttuvel Ponnuvel2Kalyani Makarand Barbadikar3Srinivas Prasad Madamshetty4Sanjeeva Rao Durbha5Hari Yadla6Sheshu Madhav Maganti7Biotechnology Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, India; Department of Biotechnology, Acharya Nagarjuna University, Guntur 522510, IndiaBiotechnology Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, IndiaHybrid Rice Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, IndiaBiotechnology Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, IndiaPlant Pathology Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, IndiaPlant Physiology Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, IndiaRegional Agricultural Research Station, Professor Jayashankar Telangana State Agricultural University, Warangal 506006, IndiaBiotechnology Division, ICAR-Indian Institute of Rice Research, Rajendra Nagar, Hyderabad 500030, India; Corresponding author.Varalu is an early maturing rice variety widely grown in the rainfed ecosystem preferred for its grain type and cooking quality. However, the yield of Varalu is substantially low since it is being affected by reproductive drought stress along with the blast disease. The genetic improvement of Varalu was done by introgressing a major yield QTL, qDTY12.1, along with two major blast resistance genes i.e. Pi54 and Pi1 through marker-assisted backcross breeding. Both traits were transferred till BC2 generation and intercrossing was followed to pyramid the two traits. Stringent foreground selection was carried out using linked markers as well as peak markers (RM28099, RM28130, RM511 and RM28163) for the targeted QTL (qDTY12.1), RM206 for Pi54 and RM224 for Pi1. Extensive background selection was done using genome-wide SSR markers. Six best lines (MSM-36, MSM-49, MSM-53, MSM-57, MSM-60 and MSM-63) having qDTY12.1 and two blast resistance genes in homozygous condition with recurrent parent genome of 95.0%-96.5% having minimal linkage drag of about 0.1 to 0.7 Mb were identified. These lines showed yield advantage under drought stress as well as irrigated conditions. MSM-36 showed better performance in the national coordinated trials conducted across India, which indicated that improved lines of Varalu expected to replace Varalu and may have an important role in sustaining rice production. The present study demonstrated the successful marker-assisted pyramiding strategy for introgression of genes/QTLs conferring biotic stress resistance and yield under abiotic stress in rice.http://www.sciencedirect.com/science/article/pii/S1672630821000688blast resistancedrought stressmarker-assisted gene pyramidingrainfed ecosystemupland rice |
| spellingShingle | Vishalakshi Balija Umakanth Bangale Senguttuvel Ponnuvel Kalyani Makarand Barbadikar Srinivas Prasad Madamshetty Sanjeeva Rao Durbha Hari Yadla Sheshu Madhav Maganti Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production Rice Science blast resistance drought stress marker-assisted gene pyramiding rainfed ecosystem upland rice |
| title | Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production |
| title_full | Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production |
| title_fullStr | Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production |
| title_full_unstemmed | Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production |
| title_short | Improvement of Upland Rice Variety by Pyramiding Drought Tolerance QTL with Two Major Blast Resistance Genes for Sustainable Rice Production |
| title_sort | improvement of upland rice variety by pyramiding drought tolerance qtl with two major blast resistance genes for sustainable rice production |
| topic | blast resistance drought stress marker-assisted gene pyramiding rainfed ecosystem upland rice |
| url | http://www.sciencedirect.com/science/article/pii/S1672630821000688 |
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