Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection
Chlamydia psittaci is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for C. psittaci infection control. In this study, an assay combining recombinase-aided amplification and a lateral flow strip (RAA-LF) for the de...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.928025/full |
| _version_ | 1818546121632382976 |
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| author | Jun Jiao Yong Qi Peisheng He Weiqiang Wan Xuan OuYang Yonghui Yu Bohai Wen Xiaolu Xiong |
| author_facet | Jun Jiao Yong Qi Peisheng He Weiqiang Wan Xuan OuYang Yonghui Yu Bohai Wen Xiaolu Xiong |
| author_sort | Jun Jiao |
| collection | DOAJ |
| description | Chlamydia psittaci is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for C. psittaci infection control. In this study, an assay combining recombinase-aided amplification and a lateral flow strip (RAA-LF) for the detection of active C. psittaci infection was developed. The RAA-LF assay targeted the CPSIT_RS02830 gene of C. psittaci and could be accomplished in 15 min at a single temperature (39°C). The analytical sensitivity of the assay was as low as 1 × 100 copies/μl and no cross-reaction with some other intracellular pathogens was observed. Moreover, all feces samples from mice infected with C. psittaci at day-1 post-infection were positive in the RAA-LF assay. In conclusion, the RAA-LF assay provides a convenient, rapid, specific and sensitive method for detection of active C. psittaci infection and it is also suitable for C. psittaci detection in field. |
| first_indexed | 2024-12-12T07:48:54Z |
| format | Article |
| id | doaj.art-95bfc62a2933444583bb40701002e15e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T07:48:54Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-95bfc62a2933444583bb40701002e15e2022-12-22T00:32:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.928025928025Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci InfectionJun Jiao0Yong Qi1Peisheng He2Weiqiang Wan3Xuan OuYang4Yonghui Yu5Bohai Wen6Xiaolu Xiong7State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaHuadong Research Institute for Medicine and Biotechniques, Nanjing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaChlamydia psittaci is the causative agent of psittacosis, a worldwide zoonotic disease. A rapid, specific, and sensitive diagnostic assay would be benefit for C. psittaci infection control. In this study, an assay combining recombinase-aided amplification and a lateral flow strip (RAA-LF) for the detection of active C. psittaci infection was developed. The RAA-LF assay targeted the CPSIT_RS02830 gene of C. psittaci and could be accomplished in 15 min at a single temperature (39°C). The analytical sensitivity of the assay was as low as 1 × 100 copies/μl and no cross-reaction with some other intracellular pathogens was observed. Moreover, all feces samples from mice infected with C. psittaci at day-1 post-infection were positive in the RAA-LF assay. In conclusion, the RAA-LF assay provides a convenient, rapid, specific and sensitive method for detection of active C. psittaci infection and it is also suitable for C. psittaci detection in field.https://www.frontiersin.org/articles/10.3389/fmicb.2022.928025/fullChlamydia psittacidetectionrecombinase-aided amplificationlateral flow stripinfection |
| spellingShingle | Jun Jiao Yong Qi Peisheng He Weiqiang Wan Xuan OuYang Yonghui Yu Bohai Wen Xiaolu Xiong Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection Frontiers in Microbiology Chlamydia psittaci detection recombinase-aided amplification lateral flow strip infection |
| title | Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection |
| title_full | Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection |
| title_fullStr | Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection |
| title_full_unstemmed | Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection |
| title_short | Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active Chlamydia psittaci Infection |
| title_sort | development of a lateral flow strip based recombinase aided amplification for active chlamydia psittaci infection |
| topic | Chlamydia psittaci detection recombinase-aided amplification lateral flow strip infection |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.928025/full |
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