Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus)
Cryopreservation of fish semen is a valuable technique for reproductive management in aquaculture. The aim of this study was to evaluate the effect of long-term storage in liquid nitrogen on post-thaw sperm functional parameters of brown-marbled grouper (Epinephelus fuscoguttatus). Samples were cryo...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342300159X |
| _version_ | 1797801720444616704 |
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| author | Xiuze Chen Haofeng Ma Sile Wu Quancheng Fu Zhenqiang Lin Sen Yang |
| author_facet | Xiuze Chen Haofeng Ma Sile Wu Quancheng Fu Zhenqiang Lin Sen Yang |
| author_sort | Xiuze Chen |
| collection | DOAJ |
| description | Cryopreservation of fish semen is a valuable technique for reproductive management in aquaculture. The aim of this study was to evaluate the effect of long-term storage in liquid nitrogen on post-thaw sperm functional parameters of brown-marbled grouper (Epinephelus fuscoguttatus). Samples were cryopreserved in a simple freezing medium (10 % DMSO + 0.3 M Glucose), sperm quality parameters were assessed at different storage periods (one month, one year, 2 years and 3 years). Our results indicated that cryostorage periods had a significant effect on the quality of frozen-thawed spermatozoa. Compared with fresh semen, cryopreserved sample was characterized by an overall decline in motility, viability, mitochondrial membrane potential (MMP), ATP content and increased level of lipid peroxidation with the storage time going on. For the analyzed quality parameters, there were no significant differences in storage time cryopreserved over one month. After 3 years of storage, frozen sperm still showed relatively high total motility, viability and MMP (all over 80 %), yielding acceptable fertilizing capacity close to that of fresh sperm. Therefore, it can be concluded that brown-marbled grouper sperm could be effectively cryopreserved for at least 3 years. The results of this study would be helpful to optimize gamete management in routine hatchery practices. |
| first_indexed | 2024-03-13T04:54:46Z |
| format | Article |
| id | doaj.art-95c03fe330dc43ccac143291f1be8556 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-13T04:54:46Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-95c03fe330dc43ccac143291f1be85562023-06-18T05:02:34ZengElsevierAquaculture Reports2352-51342023-06-0130101620Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus)Xiuze Chen0Haofeng Ma1Sile Wu2Quancheng Fu3Zhenqiang Lin4Sen Yang5College of Food Science and Technology, Guangdong Ocean University (Yangjiang Campus), Yangjiang 529500, ChinaCollege of Food Science and Technology, Guangdong Ocean University (Yangjiang Campus), Yangjiang 529500, ChinaCollege of Food Science and Technology, Guangdong Ocean University (Yangjiang Campus), Yangjiang 529500, ChinaCollege of Food Science and Technology, Guangdong Ocean University (Yangjiang Campus), Yangjiang 529500, ChinaCollege of Food Science and Technology, Guangdong Ocean University (Yangjiang Campus), Yangjiang 529500, ChinaCorresponding author.; College of Food Science and Technology, Guangdong Ocean University (Yangjiang Campus), Yangjiang 529500, ChinaCryopreservation of fish semen is a valuable technique for reproductive management in aquaculture. The aim of this study was to evaluate the effect of long-term storage in liquid nitrogen on post-thaw sperm functional parameters of brown-marbled grouper (Epinephelus fuscoguttatus). Samples were cryopreserved in a simple freezing medium (10 % DMSO + 0.3 M Glucose), sperm quality parameters were assessed at different storage periods (one month, one year, 2 years and 3 years). Our results indicated that cryostorage periods had a significant effect on the quality of frozen-thawed spermatozoa. Compared with fresh semen, cryopreserved sample was characterized by an overall decline in motility, viability, mitochondrial membrane potential (MMP), ATP content and increased level of lipid peroxidation with the storage time going on. For the analyzed quality parameters, there were no significant differences in storage time cryopreserved over one month. After 3 years of storage, frozen sperm still showed relatively high total motility, viability and MMP (all over 80 %), yielding acceptable fertilizing capacity close to that of fresh sperm. Therefore, it can be concluded that brown-marbled grouper sperm could be effectively cryopreserved for at least 3 years. The results of this study would be helpful to optimize gamete management in routine hatchery practices.http://www.sciencedirect.com/science/article/pii/S235251342300159XCryopreservationSperm qualityBrown-marbled grouperStorage time |
| spellingShingle | Xiuze Chen Haofeng Ma Sile Wu Quancheng Fu Zhenqiang Lin Sen Yang Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus) Aquaculture Reports Cryopreservation Sperm quality Brown-marbled grouper Storage time |
| title | Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus) |
| title_full | Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus) |
| title_fullStr | Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus) |
| title_full_unstemmed | Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus) |
| title_short | Evaluation of long-term cryostorage on sperm function and fertility of brown-marbled grouper (Epinephelus fuscoguttatus) |
| title_sort | evaluation of long term cryostorage on sperm function and fertility of brown marbled grouper epinephelus fuscoguttatus |
| topic | Cryopreservation Sperm quality Brown-marbled grouper Storage time |
| url | http://www.sciencedirect.com/science/article/pii/S235251342300159X |
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