Study of the Mechanism of Action of Guanxin Shutong Capsules in the Treatment of Coronary Heart Disease Based on Metabolomics
Background: Guan-Xin-Shu-Tong capsule (GXSTC) is a traditional Chinese medicine (TCM) that has been used to treat coronary heart disease (CHD) for many years in China. However, the holistic mechanism of GXSTC against CHD is still unclear. Therefore, the purpose of this paper was to systematically ex...
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.650438/full |
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| author | Dan Wang Chang Shi Zhen-Hua Ge Zhen-Hua Ge Yu-Xi Wei Yu-Xi Wei Tian-Tian Liu Yue Wang Xin-Feng Zhou Zi-Jun Yang Wei-Ting Wang Yan-Wen Zhang Xue-Hui Zhu Jun Zhang Ying Li Min Gong Xiao-Hui Wu Hong-Quan Duan |
| author_facet | Dan Wang Chang Shi Zhen-Hua Ge Zhen-Hua Ge Yu-Xi Wei Yu-Xi Wei Tian-Tian Liu Yue Wang Xin-Feng Zhou Zi-Jun Yang Wei-Ting Wang Yan-Wen Zhang Xue-Hui Zhu Jun Zhang Ying Li Min Gong Xiao-Hui Wu Hong-Quan Duan |
| author_sort | Dan Wang |
| collection | DOAJ |
| description | Background: Guan-Xin-Shu-Tong capsule (GXSTC) is a traditional Chinese medicine (TCM) that has been used to treat coronary heart disease (CHD) for many years in China. However, the holistic mechanism of GXSTC against CHD is still unclear. Therefore, the purpose of this paper was to systematically explore the mechanism of action GXSTC in the treatment of CHD rats using a metabolomics strategy.Methods: A CHD model was induced by ligation of the left anterior descending coronary artery (LAD). In each group, echocardiography was performed; the contents of creatine kinase (CK), lactate dehydrogenase (LDH) and aspartate transaminase (AST) in serum were determined; and the myocardial infarct size was measured. The metabolites in plasma were analyzed by UHPLC-MS/MS-based untargeted metabolomics. Then, multivariate statistical analysis was performed to screen potential biomarkers associated with the GXSTC treatment in the LAD-induced rat CHD model. Finally, the MetaboAnalyst 4.0 platform was used for metabolic pathway enrichment analysis.Results: GXSTC was able to regulate the contents of CK, LDH and AST; restore impaired cardiac function; and significantly reduce the myocardial infarction area in model rats. Twenty-two biomarkers and nine metabolic pathways of GXSTC in the treatment of CHD were identified through UHPLC-MS/MS-based untargeted metabolomics analysis.Conclusion: GXSTC regulates metabolic disorders of endogenous components in LAD-induced CHD rats. The anti-CHD mechanism of GXSTC is mainly related to the regulation of amino acid, lipid and hormonal metabolism. This study provides an overall view of the mechanism underlying the action of GXSTC against CHD. |
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| format | Article |
| id | doaj.art-95c5189c9aa14130adae8d07d3501121 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-17T20:50:22Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-95c5189c9aa14130adae8d07d35011212022-12-21T21:33:03ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-03-011210.3389/fphar.2021.650438650438Study of the Mechanism of Action of Guanxin Shutong Capsules in the Treatment of Coronary Heart Disease Based on MetabolomicsDan Wang0Chang Shi1Zhen-Hua Ge2Zhen-Hua Ge3Yu-Xi Wei4Yu-Xi Wei5Tian-Tian Liu6Yue Wang7Xin-Feng Zhou8Zi-Jun Yang9Wei-Ting Wang10Yan-Wen Zhang11Xue-Hui Zhu12Jun Zhang13Ying Li14Min Gong15Xiao-Hui Wu16Hong-Quan Duan17College of Pharmacy, Tianjin Medical University, Tianjin, ChinaDepartment of Pharmacy, Tianjin Huanhu Hospital, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaDepartment of Pharmacy, Tianjin Hospital, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaDepartment of Anesthesiology, Pharmacology and Therapeutics, the University of British Columbia, Vancouver, BC, CanadaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaSchool of Medical Humanities, Tianjin Medical University, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaTianjin Institute of Pharmaceutical Research, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaTianjin Neurological Institute, Tianjin Medical University, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaCollege of Pharmacy, Tianjin Medical University, Tianjin, ChinaBackground: Guan-Xin-Shu-Tong capsule (GXSTC) is a traditional Chinese medicine (TCM) that has been used to treat coronary heart disease (CHD) for many years in China. However, the holistic mechanism of GXSTC against CHD is still unclear. Therefore, the purpose of this paper was to systematically explore the mechanism of action GXSTC in the treatment of CHD rats using a metabolomics strategy.Methods: A CHD model was induced by ligation of the left anterior descending coronary artery (LAD). In each group, echocardiography was performed; the contents of creatine kinase (CK), lactate dehydrogenase (LDH) and aspartate transaminase (AST) in serum were determined; and the myocardial infarct size was measured. The metabolites in plasma were analyzed by UHPLC-MS/MS-based untargeted metabolomics. Then, multivariate statistical analysis was performed to screen potential biomarkers associated with the GXSTC treatment in the LAD-induced rat CHD model. Finally, the MetaboAnalyst 4.0 platform was used for metabolic pathway enrichment analysis.Results: GXSTC was able to regulate the contents of CK, LDH and AST; restore impaired cardiac function; and significantly reduce the myocardial infarction area in model rats. Twenty-two biomarkers and nine metabolic pathways of GXSTC in the treatment of CHD were identified through UHPLC-MS/MS-based untargeted metabolomics analysis.Conclusion: GXSTC regulates metabolic disorders of endogenous components in LAD-induced CHD rats. The anti-CHD mechanism of GXSTC is mainly related to the regulation of amino acid, lipid and hormonal metabolism. This study provides an overall view of the mechanism underlying the action of GXSTC against CHD.https://www.frontiersin.org/articles/10.3389/fphar.2021.650438/fullmetabolomicsCoronary heart diseasetraditional Chinese medicineGuan-Xin-Shu-Tong capsuleactive ingredients |
| spellingShingle | Dan Wang Chang Shi Zhen-Hua Ge Zhen-Hua Ge Yu-Xi Wei Yu-Xi Wei Tian-Tian Liu Yue Wang Xin-Feng Zhou Zi-Jun Yang Wei-Ting Wang Yan-Wen Zhang Xue-Hui Zhu Jun Zhang Ying Li Min Gong Xiao-Hui Wu Hong-Quan Duan Study of the Mechanism of Action of Guanxin Shutong Capsules in the Treatment of Coronary Heart Disease Based on Metabolomics Frontiers in Pharmacology metabolomics Coronary heart disease traditional Chinese medicine Guan-Xin-Shu-Tong capsule active ingredients |
| title | Study of the Mechanism of Action of Guanxin Shutong Capsules in the Treatment of Coronary Heart Disease Based on Metabolomics |
| title_full | Study of the Mechanism of Action of Guanxin Shutong Capsules in the Treatment of Coronary Heart Disease Based on Metabolomics |
| title_fullStr | Study of the Mechanism of Action of Guanxin Shutong Capsules in the Treatment of Coronary Heart Disease Based on Metabolomics |
| title_full_unstemmed | Study of the Mechanism of Action of Guanxin Shutong Capsules in the Treatment of Coronary Heart Disease Based on Metabolomics |
| title_short | Study of the Mechanism of Action of Guanxin Shutong Capsules in the Treatment of Coronary Heart Disease Based on Metabolomics |
| title_sort | study of the mechanism of action of guanxin shutong capsules in the treatment of coronary heart disease based on metabolomics |
| topic | metabolomics Coronary heart disease traditional Chinese medicine Guan-Xin-Shu-Tong capsule active ingredients |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.650438/full |
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