Effects of Different Additives on the Chemical Composition, Fermentation Profile, In Vitro and In Situ Digestibility of Paper mulberry Silage
Paper mulberry <i>(Broussonetia papyrifera</i>) plants are served as a local roughage in China, and they are mostly processed as silage for ruminants. This study aimed to explore the effects of different silage additives on the chemical composition, fermentation profile, as well as the i...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/8/9/435 |
| _version_ | 1797488591395356672 |
|---|---|
| author | Wei Wang Yangyi Hao Chenglong Luo Qianqian Wang Zhonghan Wang Dong Li Jing Yuan Zhijun Cao Hongjian Yang Shengli Li |
| author_facet | Wei Wang Yangyi Hao Chenglong Luo Qianqian Wang Zhonghan Wang Dong Li Jing Yuan Zhijun Cao Hongjian Yang Shengli Li |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Paper mulberry <i>(Broussonetia papyrifera</i>) plants are served as a local roughage in China, and they are mostly processed as silage for ruminants. This study aimed to explore the effects of different silage additives on the chemical composition, fermentation profile, as well as the in vitro and in situ digestibility of paper mulberry (PM) silage. Four groups consisting of PM silage, three with additives and one without any additives as the control group (CON), were established. The three experimental groups with additives were set up as follows: CON with 5 × 10<sup>6</sup> CFU per gram of fresh PM weight of lactic acid bacteria (<i>Lactobacillus plantarum</i>) (LAB); CON with 3% fresh PM weight of molasses (MOL) added to the PM silage; and CON with both LAB and MOL added (LM). After 45 days of ensiling at 20 °C, all of the PM treatment groups increased their ash content and decreased their water-soluble carbohydrate content (<i>p</i> < 0.05). Meanwhile, the pH and NH<sub>3</sub>-N content of the PM silage were lower in the additive treatment groups than in the CON group (<i>p</i> < 0.05). Lactic acid in the LM group was the highest (<i>p</i> < 0.05) among the four groups, and trace amounts of butyric acid was detected only in the CON group. In vitro dry matter digestibility was similar among all groups. Results of the in situ experiment found that the effective digestibility of the PM silage dry matter, as well as the acid detergent fiber digestibility was higher in the LM group than in the CON group (<i>p</i> < 0.05). In conclusion, the addition of LAB, MOL, and their combination can improve PM silage fermentation and improve the in situ digestibility of dry matter and acid detergent fiber; however they do not affect in the vitro digestibility of PM silage. |
| first_indexed | 2024-03-10T00:04:28Z |
| format | Article |
| id | doaj.art-95c95468b48e4af2a092c87f9a900358 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T00:04:28Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-95c95468b48e4af2a092c87f9a9003582023-11-23T16:10:38ZengMDPI AGFermentation2311-56372022-09-018943510.3390/fermentation8090435Effects of Different Additives on the Chemical Composition, Fermentation Profile, In Vitro and In Situ Digestibility of Paper mulberry SilageWei Wang0Yangyi Hao1Chenglong Luo2Qianqian Wang3Zhonghan Wang4Dong Li5Jing Yuan6Zhijun Cao7Hongjian Yang8Shengli Li9College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaPaper mulberry <i>(Broussonetia papyrifera</i>) plants are served as a local roughage in China, and they are mostly processed as silage for ruminants. This study aimed to explore the effects of different silage additives on the chemical composition, fermentation profile, as well as the in vitro and in situ digestibility of paper mulberry (PM) silage. Four groups consisting of PM silage, three with additives and one without any additives as the control group (CON), were established. The three experimental groups with additives were set up as follows: CON with 5 × 10<sup>6</sup> CFU per gram of fresh PM weight of lactic acid bacteria (<i>Lactobacillus plantarum</i>) (LAB); CON with 3% fresh PM weight of molasses (MOL) added to the PM silage; and CON with both LAB and MOL added (LM). After 45 days of ensiling at 20 °C, all of the PM treatment groups increased their ash content and decreased their water-soluble carbohydrate content (<i>p</i> < 0.05). Meanwhile, the pH and NH<sub>3</sub>-N content of the PM silage were lower in the additive treatment groups than in the CON group (<i>p</i> < 0.05). Lactic acid in the LM group was the highest (<i>p</i> < 0.05) among the four groups, and trace amounts of butyric acid was detected only in the CON group. In vitro dry matter digestibility was similar among all groups. Results of the in situ experiment found that the effective digestibility of the PM silage dry matter, as well as the acid detergent fiber digestibility was higher in the LM group than in the CON group (<i>p</i> < 0.05). In conclusion, the addition of LAB, MOL, and their combination can improve PM silage fermentation and improve the in situ digestibility of dry matter and acid detergent fiber; however they do not affect in the vitro digestibility of PM silage.https://www.mdpi.com/2311-5637/8/9/435paper mulberry silagelactic acid bacteriamolassesin vitro digestibilityin situ digestibility |
| spellingShingle | Wei Wang Yangyi Hao Chenglong Luo Qianqian Wang Zhonghan Wang Dong Li Jing Yuan Zhijun Cao Hongjian Yang Shengli Li Effects of Different Additives on the Chemical Composition, Fermentation Profile, In Vitro and In Situ Digestibility of Paper mulberry Silage Fermentation paper mulberry silage lactic acid bacteria molasses in vitro digestibility in situ digestibility |
| title | Effects of Different Additives on the Chemical Composition, Fermentation Profile, In Vitro and In Situ Digestibility of Paper mulberry Silage |
| title_full | Effects of Different Additives on the Chemical Composition, Fermentation Profile, In Vitro and In Situ Digestibility of Paper mulberry Silage |
| title_fullStr | Effects of Different Additives on the Chemical Composition, Fermentation Profile, In Vitro and In Situ Digestibility of Paper mulberry Silage |
| title_full_unstemmed | Effects of Different Additives on the Chemical Composition, Fermentation Profile, In Vitro and In Situ Digestibility of Paper mulberry Silage |
| title_short | Effects of Different Additives on the Chemical Composition, Fermentation Profile, In Vitro and In Situ Digestibility of Paper mulberry Silage |
| title_sort | effects of different additives on the chemical composition fermentation profile in vitro and in situ digestibility of paper mulberry silage |
| topic | paper mulberry silage lactic acid bacteria molasses in vitro digestibility in situ digestibility |
| url | https://www.mdpi.com/2311-5637/8/9/435 |
| work_keys_str_mv | AT weiwang effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage AT yangyihao effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage AT chenglongluo effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage AT qianqianwang effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage AT zhonghanwang effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage AT dongli effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage AT jingyuan effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage AT zhijuncao effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage AT hongjianyang effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage AT shenglili effectsofdifferentadditivesonthechemicalcompositionfermentationprofileinvitroandinsitudigestibilityofpapermulberrysilage |