Nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate-induced airway inflammation in horses
The objective of this study was to determine the extent that nebulized glycosylated caffeic acid phenylether ester-4-O-alpha-D-glucopyranoside (G-CAPE) attenuates particulate-induced airway inflammation in healthy horses. Our hypothesis was that nebulization with G-CAPE would result in improved resp...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.958567/full |
| _version_ | 1797990398351638528 |
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| author | Jessica J. Rutledge Jillian Paegelow Jerry Ritchey Anuradha Singh Theresa Rizzi Cynthia Murray Lyndi Gilliam Evan Crisman Natasha J. Williams Todd C. Holbrook |
| author_facet | Jessica J. Rutledge Jillian Paegelow Jerry Ritchey Anuradha Singh Theresa Rizzi Cynthia Murray Lyndi Gilliam Evan Crisman Natasha J. Williams Todd C. Holbrook |
| author_sort | Jessica J. Rutledge |
| collection | DOAJ |
| description | The objective of this study was to determine the extent that nebulized glycosylated caffeic acid phenylether ester-4-O-alpha-D-glucopyranoside (G-CAPE) attenuates particulate-induced airway inflammation in healthy horses. Our hypothesis was that nebulization with G-CAPE would result in improved respiratory scores, higher arterial oxygen partial pressure, and less inflammatory airway infiltrates in horses with induced airway inflammation, compared with untreated controls. Five healthy adult horses were housed inside a climate controlled, closed barn on straw bedding and fed ad lib moldy grass hay for 16 days to induce airway inflammation. An experimental crossover study was performed in which animals were treated with 200 mg G-CAPE dissolved in 45 mL of 10% triethanolamine (G-CAPE group) or 45 mL of 10% triethanolamine (CONTROL group), and clinical respiratory scoring, arterial blood gases, and bronchoalveolar lavages (BALs) were collected at predetermined time points up to 24 h post nebulization. While the mean neutrophil percentage decreased in treated horses compared to controls (9.3 ± 2.0 and 16.9 ± 2.4, respectively) at 6 hours post treatment (t = 6 h), the difference did not achieve statistical significance (p = 0.1154). Blood gas analysis did not differ significantly between groups. There was a significant difference in the mean respiratory scores of G-CAPE-treated horses between baseline and at 1-h post treatment (from 3.2 ± 0.7 to 1.6 ± 0.7, p = 0.0013). This study demonstrates that a single nebulized dose of G-CAPE decreased clinical respiratory scores 1 h post administration and decreased BAL percentage of neutrophils 6 h post administration in horses with particulate induced airway inflammation. This compound shows promise as an anti-inflammatory and warrants further investigation. |
| first_indexed | 2024-04-11T08:34:36Z |
| format | Article |
| id | doaj.art-95ce40962bf747adaf91e3a1020cc008 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-11T08:34:36Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-95ce40962bf747adaf91e3a1020cc0082022-12-22T04:34:22ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-11-01910.3389/fvets.2022.958567958567Nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate-induced airway inflammation in horsesJessica J. Rutledge0Jillian Paegelow1Jerry Ritchey2Anuradha Singh3Theresa Rizzi4Cynthia Murray5Lyndi Gilliam6Evan Crisman7Natasha J. Williams8Todd C. Holbrook9Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, United StatesDepartment of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, United StatesDepartment of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK, United StatesDepartment of Chemistry, Oklahoma State University, Stillwater, OK, United StatesDepartment of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK, United StatesDepartment of Mathematics and Statistics, University of Central Oklahoma, Edmond, OK, United StatesDepartment of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, United StatesDepartment of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, United StatesDepartment of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, United StatesDepartment of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, United StatesThe objective of this study was to determine the extent that nebulized glycosylated caffeic acid phenylether ester-4-O-alpha-D-glucopyranoside (G-CAPE) attenuates particulate-induced airway inflammation in healthy horses. Our hypothesis was that nebulization with G-CAPE would result in improved respiratory scores, higher arterial oxygen partial pressure, and less inflammatory airway infiltrates in horses with induced airway inflammation, compared with untreated controls. Five healthy adult horses were housed inside a climate controlled, closed barn on straw bedding and fed ad lib moldy grass hay for 16 days to induce airway inflammation. An experimental crossover study was performed in which animals were treated with 200 mg G-CAPE dissolved in 45 mL of 10% triethanolamine (G-CAPE group) or 45 mL of 10% triethanolamine (CONTROL group), and clinical respiratory scoring, arterial blood gases, and bronchoalveolar lavages (BALs) were collected at predetermined time points up to 24 h post nebulization. While the mean neutrophil percentage decreased in treated horses compared to controls (9.3 ± 2.0 and 16.9 ± 2.4, respectively) at 6 hours post treatment (t = 6 h), the difference did not achieve statistical significance (p = 0.1154). Blood gas analysis did not differ significantly between groups. There was a significant difference in the mean respiratory scores of G-CAPE-treated horses between baseline and at 1-h post treatment (from 3.2 ± 0.7 to 1.6 ± 0.7, p = 0.0013). This study demonstrates that a single nebulized dose of G-CAPE decreased clinical respiratory scores 1 h post administration and decreased BAL percentage of neutrophils 6 h post administration in horses with particulate induced airway inflammation. This compound shows promise as an anti-inflammatory and warrants further investigation.https://www.frontiersin.org/articles/10.3389/fvets.2022.958567/fullequine asthmaneutrophiliaairway inflammationnebulizationparticulateshay |
| spellingShingle | Jessica J. Rutledge Jillian Paegelow Jerry Ritchey Anuradha Singh Theresa Rizzi Cynthia Murray Lyndi Gilliam Evan Crisman Natasha J. Williams Todd C. Holbrook Nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate-induced airway inflammation in horses Frontiers in Veterinary Science equine asthma neutrophilia airway inflammation nebulization particulates hay |
| title | Nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate-induced airway inflammation in horses |
| title_full | Nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate-induced airway inflammation in horses |
| title_fullStr | Nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate-induced airway inflammation in horses |
| title_full_unstemmed | Nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate-induced airway inflammation in horses |
| title_short | Nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate-induced airway inflammation in horses |
| title_sort | nebulized glycosylated caffeic acid phenylether ester attenuation of environmental particulate induced airway inflammation in horses |
| topic | equine asthma neutrophilia airway inflammation nebulization particulates hay |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.958567/full |
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