Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced Osteoclastogenesis
Changes in mitochondrial bioenergetics are believed to take place during osteoclastogenesis. This study aims to assess changes in mitochondrial bioenergetics and reactive oxygen species (ROS) levels during polyethylene (PE)-induced osteoclastogenesis in vitro. For this purpose, RAW264.7 cells were c...
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2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| author | Nur Shukriyah Mohamad Hazir Nor Hamdan Mohamad Yahaya Muhamad Syahrul Fitri Zawawi Hanafi Ahmad Damanhuri Norazlina Mohamed Ekram Alias |
| author_facet | Nur Shukriyah Mohamad Hazir Nor Hamdan Mohamad Yahaya Muhamad Syahrul Fitri Zawawi Hanafi Ahmad Damanhuri Norazlina Mohamed Ekram Alias |
| author_sort | Nur Shukriyah Mohamad Hazir |
| collection | DOAJ |
| description | Changes in mitochondrial bioenergetics are believed to take place during osteoclastogenesis. This study aims to assess changes in mitochondrial bioenergetics and reactive oxygen species (ROS) levels during polyethylene (PE)-induced osteoclastogenesis in vitro. For this purpose, RAW264.7 cells were cultured for nine days and allowed to differentiate into osteoclasts in the presence of PE and RANKL. The total TRAP-positive cells, resorption activity, expression of osteoclast marker genes, ROS level, mitochondrial bioenergetics, glycolysis, and substrate utilization were measured. The effect of tocotrienols-rich fraction (TRF) treatment (50 ng/mL) on those parameters during PE-induced osteoclastogenesis was also studied. During PE-induced osteoclastogenesis, as depicted by an increase in TRAP-positive cells and gene expression of osteoclast-related markers, higher proton leak, higher extracellular acidification rate (ECAR), as well as higher levels of ROS and NADPH oxidases (NOXs) were observed in the differentiated cells. The oxidation level of some substrates in the differentiated group was higher than in other groups. TRF treatment significantly reduced the number of TRAP-positive osteoclasts, bone resorption activity, and ROS levels, as well as modulating the gene expression of antioxidant-related genes and mitochondrial function. In conclusion, changes in mitochondrial bioenergetics and substrate utilization were observed during PE-induced osteoclastogenesis, while TRF treatment modulated these changes. |
| first_indexed | 2024-03-09T12:35:06Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:35:06Z |
| publishDate | 2022-07-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-95e0d7de21b849dd9080095faf8b977d2023-11-30T22:25:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012315833110.3390/ijms23158331Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced OsteoclastogenesisNur Shukriyah Mohamad Hazir0Nor Hamdan Mohamad Yahaya1Muhamad Syahrul Fitri Zawawi2Hanafi Ahmad Damanhuri3Norazlina Mohamed4Ekram Alias5Department of Biochemistry, Faculty of Medicine, Pusat Perubatan Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaDepartment of Orthopaedics, Faculty of Medicine, Pusat Perubatan Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaDepartment of Orthopaedics, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kota Bharu 16150, Kelantan, MalaysiaDepartment of Biochemistry, Faculty of Medicine, Pusat Perubatan Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Pusat Perubatan Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaDepartment of Biochemistry, Faculty of Medicine, Pusat Perubatan Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur 56000, MalaysiaChanges in mitochondrial bioenergetics are believed to take place during osteoclastogenesis. This study aims to assess changes in mitochondrial bioenergetics and reactive oxygen species (ROS) levels during polyethylene (PE)-induced osteoclastogenesis in vitro. For this purpose, RAW264.7 cells were cultured for nine days and allowed to differentiate into osteoclasts in the presence of PE and RANKL. The total TRAP-positive cells, resorption activity, expression of osteoclast marker genes, ROS level, mitochondrial bioenergetics, glycolysis, and substrate utilization were measured. The effect of tocotrienols-rich fraction (TRF) treatment (50 ng/mL) on those parameters during PE-induced osteoclastogenesis was also studied. During PE-induced osteoclastogenesis, as depicted by an increase in TRAP-positive cells and gene expression of osteoclast-related markers, higher proton leak, higher extracellular acidification rate (ECAR), as well as higher levels of ROS and NADPH oxidases (NOXs) were observed in the differentiated cells. The oxidation level of some substrates in the differentiated group was higher than in other groups. TRF treatment significantly reduced the number of TRAP-positive osteoclasts, bone resorption activity, and ROS levels, as well as modulating the gene expression of antioxidant-related genes and mitochondrial function. In conclusion, changes in mitochondrial bioenergetics and substrate utilization were observed during PE-induced osteoclastogenesis, while TRF treatment modulated these changes.https://www.mdpi.com/1422-0067/23/15/8331polyethylenepolyethylene-induced osteolysisosteoclastosteoclastogenesisreactive oxygen speciesmitochondrial bioenergetics |
| spellingShingle | Nur Shukriyah Mohamad Hazir Nor Hamdan Mohamad Yahaya Muhamad Syahrul Fitri Zawawi Hanafi Ahmad Damanhuri Norazlina Mohamed Ekram Alias Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced Osteoclastogenesis International Journal of Molecular Sciences polyethylene polyethylene-induced osteolysis osteoclast osteoclastogenesis reactive oxygen species mitochondrial bioenergetics |
| title | Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced Osteoclastogenesis |
| title_full | Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced Osteoclastogenesis |
| title_fullStr | Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced Osteoclastogenesis |
| title_full_unstemmed | Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced Osteoclastogenesis |
| title_short | Changes in Metabolism and Mitochondrial Bioenergetics during Polyethylene-Induced Osteoclastogenesis |
| title_sort | changes in metabolism and mitochondrial bioenergetics during polyethylene induced osteoclastogenesis |
| topic | polyethylene polyethylene-induced osteolysis osteoclast osteoclastogenesis reactive oxygen species mitochondrial bioenergetics |
| url | https://www.mdpi.com/1422-0067/23/15/8331 |
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