The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor
In the modern farming industry, the irrational or illegal use of veterinary drugs leads to residues in animal-derived food, which can seriously threaten human health. Efficient detection of low concentrations of drug residues in animal products in a short time is a key challenge for analytical metho...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/8/578 |
| _version_ | 1797432378692468736 |
|---|---|
| author | Jiaxu Xiao Nana Wei Shuangmin Wu Huaming Li Xiaoyang Yin Yu Si Long Li Dapeng Peng |
| author_facet | Jiaxu Xiao Nana Wei Shuangmin Wu Huaming Li Xiaoyang Yin Yu Si Long Li Dapeng Peng |
| author_sort | Jiaxu Xiao |
| collection | DOAJ |
| description | In the modern farming industry, the irrational or illegal use of veterinary drugs leads to residues in animal-derived food, which can seriously threaten human health. Efficient detection of low concentrations of drug residues in animal products in a short time is a key challenge for analytical methods. This study proposes to use an antibody chip biosensor for rapid and automated analysis of cephalosporins, aminoglycosides, and sulfonamide antibiotics in pork and milk. 3D polymer slides were applied for the preparation of antibody chips. Ovalbumin (OVA) or bovine serum albumin (BSA) conjugates of the haptens were immobilized as spots on disposable chips. Monoclonal antibodies (mAbs) against cefalexin, ceftiofur, gentamicin, neomycin, and sulfonamides allowed the simultaneous detection of the respective analytes. Antibody binding was detected by a second antibody labeled with Cy3-generating fluorescence, which was scanned a with chip scanner. The limits of detection (LOD) for all the analytes were far below the respective maximum residue limits (MRLs) and ranged from 0.51 to 4.3 µg/kg. The average recoveries of all the analytes in each sample were in the range of 81.6–113.6%. The intra- and inter-assay CV was less than 12.9% and showed good accuracy and precision for all the antibiotics at the MRL level. The sample pretreatment method is simple, and the results are confirmed to be accurate by LC–MS/MS; therefore, this method is valuable for the quality control of animal-derived food. |
| first_indexed | 2024-03-09T10:00:35Z |
| format | Article |
| id | doaj.art-95e0f8054b9f47e5b478a13d9249fad4 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T10:00:35Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-95e0f8054b9f47e5b478a13d9249fad42023-12-01T23:29:58ZengMDPI AGBiosensors2079-63742022-07-0112857810.3390/bios12080578The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip BiosensorJiaxu Xiao0Nana Wei1Shuangmin Wu2Huaming Li3Xiaoyang Yin4Yu Si5Long Li6Dapeng Peng7National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, ChinaKunpeng Institute of Modern Agriculture at Foshan, Foshan 528200, ChinaNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, ChinaNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, ChinaNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, ChinaNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, ChinaNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, ChinaNational Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, ChinaIn the modern farming industry, the irrational or illegal use of veterinary drugs leads to residues in animal-derived food, which can seriously threaten human health. Efficient detection of low concentrations of drug residues in animal products in a short time is a key challenge for analytical methods. This study proposes to use an antibody chip biosensor for rapid and automated analysis of cephalosporins, aminoglycosides, and sulfonamide antibiotics in pork and milk. 3D polymer slides were applied for the preparation of antibody chips. Ovalbumin (OVA) or bovine serum albumin (BSA) conjugates of the haptens were immobilized as spots on disposable chips. Monoclonal antibodies (mAbs) against cefalexin, ceftiofur, gentamicin, neomycin, and sulfonamides allowed the simultaneous detection of the respective analytes. Antibody binding was detected by a second antibody labeled with Cy3-generating fluorescence, which was scanned a with chip scanner. The limits of detection (LOD) for all the analytes were far below the respective maximum residue limits (MRLs) and ranged from 0.51 to 4.3 µg/kg. The average recoveries of all the analytes in each sample were in the range of 81.6–113.6%. The intra- and inter-assay CV was less than 12.9% and showed good accuracy and precision for all the antibiotics at the MRL level. The sample pretreatment method is simple, and the results are confirmed to be accurate by LC–MS/MS; therefore, this method is valuable for the quality control of animal-derived food.https://www.mdpi.com/2079-6374/12/8/578multi-residuesantibody chipcephalosporinsaminoglycosidessulfonamides |
| spellingShingle | Jiaxu Xiao Nana Wei Shuangmin Wu Huaming Li Xiaoyang Yin Yu Si Long Li Dapeng Peng The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor Biosensors multi-residues antibody chip cephalosporins aminoglycosides sulfonamides |
| title | The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor |
| title_full | The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor |
| title_fullStr | The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor |
| title_full_unstemmed | The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor |
| title_short | The Simultaneous Detection of Multiple Antibiotics in Milk and Pork Based on an Antibody Chip Biosensor |
| title_sort | simultaneous detection of multiple antibiotics in milk and pork based on an antibody chip biosensor |
| topic | multi-residues antibody chip cephalosporins aminoglycosides sulfonamides |
| url | https://www.mdpi.com/2079-6374/12/8/578 |
| work_keys_str_mv | AT jiaxuxiao thesimultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT nanawei thesimultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT shuangminwu thesimultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT huamingli thesimultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT xiaoyangyin thesimultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT yusi thesimultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT longli thesimultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT dapengpeng thesimultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT jiaxuxiao simultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT nanawei simultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT shuangminwu simultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT huamingli simultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT xiaoyangyin simultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT yusi simultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT longli simultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor AT dapengpeng simultaneousdetectionofmultipleantibioticsinmilkandporkbasedonanantibodychipbiosensor |