Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine
Kidney xenotransplantation has been attracting attention as a treatment option for end-stage renal disease. Fetal porcine kidneys are particularly promising grafts because they can reduce rejection through vascularization from host vessels. We are proposing xenogeneic regenerative medicine using fet...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/12/6/2293 |
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| author | Kenji Matsui Yoshitaka Kinoshita Yuka Inage Naoto Matsumoto Keita Morimoto Yatsumu Saito Tsuyoshi Takamura Hitomi Matsunari Shuichiro Yamanaka Hiroshi Nagashima Eiji Kobayashi Takashi Yokoo |
| author_facet | Kenji Matsui Yoshitaka Kinoshita Yuka Inage Naoto Matsumoto Keita Morimoto Yatsumu Saito Tsuyoshi Takamura Hitomi Matsunari Shuichiro Yamanaka Hiroshi Nagashima Eiji Kobayashi Takashi Yokoo |
| author_sort | Kenji Matsui |
| collection | DOAJ |
| description | Kidney xenotransplantation has been attracting attention as a treatment option for end-stage renal disease. Fetal porcine kidneys are particularly promising grafts because they can reduce rejection through vascularization from host vessels. We are proposing xenogeneic regenerative medicine using fetal porcine kidneys injected with human nephron progenitor cells. For clinical application, it is desirable to establish reliable methods for the preservation and quality assessment of grafts. We evaluated the differentiation potency of vitrified porcine fetal kidneys compared with nonfrozen kidneys, using an in vivo differentiation model. Fetal porcine kidneys connected to the bladder were frozen via vitrification and stored in liquid nitrogen. Several days later, they were thawed and transplanted under the retroperitoneum of immunocompromised mice. After 14 days, the frozen kidneys grew and differentiated into mature nephrons, and the findings were comparable to those of nonfrozen kidneys. In conclusion, we demonstrated that the differentiation potency of vitrified fetal porcine kidneys could be evaluated using this model, thereby providing a practical protocol to assess the quality of individual lots. |
| first_indexed | 2024-03-11T06:22:14Z |
| format | Article |
| id | doaj.art-95e3d85e9a094e35bc26c52138e33405 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-11T06:22:14Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-95e3d85e9a094e35bc26c52138e334052023-11-17T11:50:50ZengMDPI AGJournal of Clinical Medicine2077-03832023-03-01126229310.3390/jcm12062293Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative MedicineKenji Matsui0Yoshitaka Kinoshita1Yuka Inage2Naoto Matsumoto3Keita Morimoto4Yatsumu Saito5Tsuyoshi Takamura6Hitomi Matsunari7Shuichiro Yamanaka8Hiroshi Nagashima9Eiji Kobayashi10Takashi Yokoo11Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanMeiji University International Institute for Bio-Resource Research, Kanagawa 214-8571, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanMeiji University International Institute for Bio-Resource Research, Kanagawa 214-8571, JapanDepartment of Kidney Regenerative Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanDivision of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, JapanKidney xenotransplantation has been attracting attention as a treatment option for end-stage renal disease. Fetal porcine kidneys are particularly promising grafts because they can reduce rejection through vascularization from host vessels. We are proposing xenogeneic regenerative medicine using fetal porcine kidneys injected with human nephron progenitor cells. For clinical application, it is desirable to establish reliable methods for the preservation and quality assessment of grafts. We evaluated the differentiation potency of vitrified porcine fetal kidneys compared with nonfrozen kidneys, using an in vivo differentiation model. Fetal porcine kidneys connected to the bladder were frozen via vitrification and stored in liquid nitrogen. Several days later, they were thawed and transplanted under the retroperitoneum of immunocompromised mice. After 14 days, the frozen kidneys grew and differentiated into mature nephrons, and the findings were comparable to those of nonfrozen kidneys. In conclusion, we demonstrated that the differentiation potency of vitrified fetal porcine kidneys could be evaluated using this model, thereby providing a practical protocol to assess the quality of individual lots.https://www.mdpi.com/2077-0383/12/6/2293fetal pigkidney xenotransplantationvitrificationorgan preservation |
| spellingShingle | Kenji Matsui Yoshitaka Kinoshita Yuka Inage Naoto Matsumoto Keita Morimoto Yatsumu Saito Tsuyoshi Takamura Hitomi Matsunari Shuichiro Yamanaka Hiroshi Nagashima Eiji Kobayashi Takashi Yokoo Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine Journal of Clinical Medicine fetal pig kidney xenotransplantation vitrification organ preservation |
| title | Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine |
| title_full | Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine |
| title_fullStr | Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine |
| title_full_unstemmed | Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine |
| title_short | Cryopreservation of Fetal Porcine Kidneys for Xenogeneic Regenerative Medicine |
| title_sort | cryopreservation of fetal porcine kidneys for xenogeneic regenerative medicine |
| topic | fetal pig kidney xenotransplantation vitrification organ preservation |
| url | https://www.mdpi.com/2077-0383/12/6/2293 |
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