Long Dan Xie Gan Formula Granule Promotes Pro-Inflammatory Cytokine Expression in Female Guinea Pigs with Recurrent Genital Herpes
ABSTRACT: Objective To explore the effects of Long Dan Xie Gan formula granule (LDXGFG) on regulation of pro-inflammatory cytokines in female guinea pigs with recurrent genital herpes (herpes simplex virus 2, HSV-2).Methods Levels of pro-inflammatory cytokines in blood of HSV-2-infected guinea pigs,...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2018-06-01
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| Series: | Digital Chinese Medicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589377719300217 |
| _version_ | 1818133742901788672 |
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| author | Lin KUANG En-Hui HUANG Qing-Hu HE Shao-Wu CHENG Xiao-Dan LIU |
| author_facet | Lin KUANG En-Hui HUANG Qing-Hu HE Shao-Wu CHENG Xiao-Dan LIU |
| author_sort | Lin KUANG |
| collection | DOAJ |
| description | ABSTRACT: Objective To explore the effects of Long Dan Xie Gan formula granule (LDXGFG) on regulation of pro-inflammatory cytokines in female guinea pigs with recurrent genital herpes (herpes simplex virus 2, HSV-2).Methods Levels of pro-inflammatory cytokines in blood of HSV-2-infected guinea pigs, including IL-6, IL-8, IL-10, IL-12, IFN-α, IFN-β, IFN-γ, and TNF-α, were detected by ELISA; corresponding gene expression levels in tissues were detected by real-time PCR.Results IL-6, IL-10, IL-12, IFN-α, IFN-γ and TNF-α decreased significantly in both blood and diseased tissues after infection with recurrent genital herpes. Upon feeding LDXGFG to HSV-2-infected guinea pigs, IL-6, IL-10, IL-12, IFN-α, IFN-γ and TNF-α demonstrated significant increases, similar to the effects of acyclovir (ACV). LDXGFG promoted the expression of pro-inflammatory cytokines in blood and tissue, with a stronger effect than ACV. Moreover, LDXGFG demonstrated broader effects than ACV.Conclusion The present results suggest that LDXGFG can serve as an alternative, inexpensive, and long-term treatment for HSV-2 infection. Keywords: Recurrent genital herpes, HSV-2, Long Dan Xie Gan formula granule, (LDXGFG), Pro-inflammatory cytokines, ACV, Guinea pig |
| first_indexed | 2024-12-11T08:57:34Z |
| format | Article |
| id | doaj.art-95e5a60b28814aaa86930c6fc8c8ff39 |
| institution | Directory Open Access Journal |
| issn | 2589-3777 |
| language | English |
| last_indexed | 2024-12-11T08:57:34Z |
| publishDate | 2018-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Digital Chinese Medicine |
| spelling | doaj.art-95e5a60b28814aaa86930c6fc8c8ff392022-12-22T01:13:51ZengKeAi Communications Co., Ltd.Digital Chinese Medicine2589-37772018-06-0112164172Long Dan Xie Gan Formula Granule Promotes Pro-Inflammatory Cytokine Expression in Female Guinea Pigs with Recurrent Genital HerpesLin KUANG0En-Hui HUANG1Qing-Hu HE2Shao-Wu CHENG3Xiao-Dan LIU4Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; Corresponding author: Lin KUANG, Associate Professor, M. D.. Research direction: Infectious Dermotology. Peer review under the responsibility of Hunan University of Chinese Medicine.Hunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaHunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaHunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaHunan University of Chinese Medicine, Changsha, Hunan 410208, ChinaABSTRACT: Objective To explore the effects of Long Dan Xie Gan formula granule (LDXGFG) on regulation of pro-inflammatory cytokines in female guinea pigs with recurrent genital herpes (herpes simplex virus 2, HSV-2).Methods Levels of pro-inflammatory cytokines in blood of HSV-2-infected guinea pigs, including IL-6, IL-8, IL-10, IL-12, IFN-α, IFN-β, IFN-γ, and TNF-α, were detected by ELISA; corresponding gene expression levels in tissues were detected by real-time PCR.Results IL-6, IL-10, IL-12, IFN-α, IFN-γ and TNF-α decreased significantly in both blood and diseased tissues after infection with recurrent genital herpes. Upon feeding LDXGFG to HSV-2-infected guinea pigs, IL-6, IL-10, IL-12, IFN-α, IFN-γ and TNF-α demonstrated significant increases, similar to the effects of acyclovir (ACV). LDXGFG promoted the expression of pro-inflammatory cytokines in blood and tissue, with a stronger effect than ACV. Moreover, LDXGFG demonstrated broader effects than ACV.Conclusion The present results suggest that LDXGFG can serve as an alternative, inexpensive, and long-term treatment for HSV-2 infection. Keywords: Recurrent genital herpes, HSV-2, Long Dan Xie Gan formula granule, (LDXGFG), Pro-inflammatory cytokines, ACV, Guinea pighttp://www.sciencedirect.com/science/article/pii/S2589377719300217 |
| spellingShingle | Lin KUANG En-Hui HUANG Qing-Hu HE Shao-Wu CHENG Xiao-Dan LIU Long Dan Xie Gan Formula Granule Promotes Pro-Inflammatory Cytokine Expression in Female Guinea Pigs with Recurrent Genital Herpes Digital Chinese Medicine |
| title | Long Dan Xie Gan Formula Granule Promotes Pro-Inflammatory Cytokine Expression in Female Guinea Pigs with Recurrent Genital Herpes |
| title_full | Long Dan Xie Gan Formula Granule Promotes Pro-Inflammatory Cytokine Expression in Female Guinea Pigs with Recurrent Genital Herpes |
| title_fullStr | Long Dan Xie Gan Formula Granule Promotes Pro-Inflammatory Cytokine Expression in Female Guinea Pigs with Recurrent Genital Herpes |
| title_full_unstemmed | Long Dan Xie Gan Formula Granule Promotes Pro-Inflammatory Cytokine Expression in Female Guinea Pigs with Recurrent Genital Herpes |
| title_short | Long Dan Xie Gan Formula Granule Promotes Pro-Inflammatory Cytokine Expression in Female Guinea Pigs with Recurrent Genital Herpes |
| title_sort | long dan xie gan formula granule promotes pro inflammatory cytokine expression in female guinea pigs with recurrent genital herpes |
| url | http://www.sciencedirect.com/science/article/pii/S2589377719300217 |
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