Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors
Skeletal muscle mass is decreased under a wide range of pathologic conditions. In particular, chemotherapy is well known for inducing muscle loss and atrophy. Previous studies using tonsil-derived mesenchymal stem cells (T-MSCs) or a T-MSC-conditioned medium showed effective recovery of total body w...
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MDPI AG
2021-08-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/8/2169 |
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| author | Kyung-Ah Cho Da-Won Choi Yu-Hee Kim Jungwoo Kim Kyung-Ha Ryu So-Youn Woo |
| author_facet | Kyung-Ah Cho Da-Won Choi Yu-Hee Kim Jungwoo Kim Kyung-Ha Ryu So-Youn Woo |
| author_sort | Kyung-Ah Cho |
| collection | DOAJ |
| description | Skeletal muscle mass is decreased under a wide range of pathologic conditions. In particular, chemotherapy is well known for inducing muscle loss and atrophy. Previous studies using tonsil-derived mesenchymal stem cells (T-MSCs) or a T-MSC-conditioned medium showed effective recovery of total body weight in the chemotherapy-preconditioned bone marrow transplantation mouse model. This study investigated whether extracellular vesicles of T-MSCs, such as exosomes, are a key player in the recovery of body weight and skeletal muscle mass in chemotherapy-treated mice. T-MSC exosomes transplantation significantly decreased loss of total body weight and muscle mass in the busulfan-cyclophosphamide conditioning regimen in BALB/c recipient mice containing elevated serum activin A. Additionally, T-MSC exosomes rescued impaired C2C12 cell differentiation in the presence of activin A in vitro. We found that T-MSC exosomes possess abundant miR-145-5p, which targets activin A receptors, ACVR2A, and ACVR1B. Indeed, T-MSC exosomes rescue muscle atrophy both in vivo and in vitro via miR-145-5p dependent manner. These results suggest that T-MSC exosomes have therapeutic potential to maintain or improve skeletal muscle mass in various activin A elevated pathologic conditions. |
| first_indexed | 2024-03-10T08:54:42Z |
| format | Article |
| id | doaj.art-95e896fcc5d240fcb809c4d3c47c92ee |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T08:54:42Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-95e896fcc5d240fcb809c4d3c47c92ee2023-11-22T07:12:43ZengMDPI AGCells2073-44092021-08-01108216910.3390/cells10082169Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A ReceptorsKyung-Ah Cho0Da-Won Choi1Yu-Hee Kim2Jungwoo Kim3Kyung-Ha Ryu4So-Youn Woo5Department of Microbiology, College of Medicine, Ewha Womans University, Seoul 07804, KoreaDepartment of Microbiology, College of Medicine, Ewha Womans University, Seoul 07804, KoreaAdvanced Biomedical Research Institute, Ewha Womans University Seoul Hospital, Seoul 07804, KoreaDepartment of Microbiology, College of Medicine, Ewha Womans University, Seoul 07804, KoreaDepartment of Pediatrics, College of Medicine, Ewha Womans University, Seoul 07804, KoreaDepartment of Microbiology, College of Medicine, Ewha Womans University, Seoul 07804, KoreaSkeletal muscle mass is decreased under a wide range of pathologic conditions. In particular, chemotherapy is well known for inducing muscle loss and atrophy. Previous studies using tonsil-derived mesenchymal stem cells (T-MSCs) or a T-MSC-conditioned medium showed effective recovery of total body weight in the chemotherapy-preconditioned bone marrow transplantation mouse model. This study investigated whether extracellular vesicles of T-MSCs, such as exosomes, are a key player in the recovery of body weight and skeletal muscle mass in chemotherapy-treated mice. T-MSC exosomes transplantation significantly decreased loss of total body weight and muscle mass in the busulfan-cyclophosphamide conditioning regimen in BALB/c recipient mice containing elevated serum activin A. Additionally, T-MSC exosomes rescued impaired C2C12 cell differentiation in the presence of activin A in vitro. We found that T-MSC exosomes possess abundant miR-145-5p, which targets activin A receptors, ACVR2A, and ACVR1B. Indeed, T-MSC exosomes rescue muscle atrophy both in vivo and in vitro via miR-145-5p dependent manner. These results suggest that T-MSC exosomes have therapeutic potential to maintain or improve skeletal muscle mass in various activin A elevated pathologic conditions.https://www.mdpi.com/2073-4409/10/8/2169mesenchymal stem cellexosomesskeletal muscleactivin A |
| spellingShingle | Kyung-Ah Cho Da-Won Choi Yu-Hee Kim Jungwoo Kim Kyung-Ha Ryu So-Youn Woo Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors Cells mesenchymal stem cell exosomes skeletal muscle activin A |
| title | Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors |
| title_full | Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors |
| title_fullStr | Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors |
| title_full_unstemmed | Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors |
| title_short | Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors |
| title_sort | mesenchymal stem cell derived exosomes protect muscle loss by mir 145 5p activity targeting activin a receptors |
| topic | mesenchymal stem cell exosomes skeletal muscle activin A |
| url | https://www.mdpi.com/2073-4409/10/8/2169 |
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