Potential Anti-Tumor Activity of Nardoguaianone L Isolated from <i>Nardostachys jatamansi</i> DC. in SW1990 Cells
Natural products (NPs) were a rich source of diverse bioactive molecules. Most anti-tumor agents were built on natural scaffolds. <i>Nardostachys jatamansi</i> DC. was an important plant used to process the traditional Chinese herbal medicines “gansong”. Pancreatic cancer was the fourth...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/21/7490 |
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| author | Chun-Yan Sang Yi-Dan Zheng Li-Mei Ma Kai Wang Cheng-Bo Wang Tian Chai Komila A. Eshbakova Jun-Li Yang |
| author_facet | Chun-Yan Sang Yi-Dan Zheng Li-Mei Ma Kai Wang Cheng-Bo Wang Tian Chai Komila A. Eshbakova Jun-Li Yang |
| author_sort | Chun-Yan Sang |
| collection | DOAJ |
| description | Natural products (NPs) were a rich source of diverse bioactive molecules. Most anti-tumor agents were built on natural scaffolds. <i>Nardostachys jatamansi</i> DC. was an important plant used to process the traditional Chinese herbal medicines “gansong”. Pancreatic cancer was the fourth most common cause of cancer-related death in the world. Hence, there was an urgent need to develop novel agents for the treatment of pancreatic cancer. In this paper, nardoguaianone L (G-6) is isolated from <i>N. jatamansi</i>, which inhibited SW1990 cells colony formation and cell migration, and induced cell apoptosis. Furthermore, we analyzed the differential expression proteins after treatment with G-6 in SW1990 cells by using iTRAQ/TMT-based quantitative proteomics technology, and the results showed that G-6 regulated 143 proteins’ differential expression by GO annotation, including biological process, cellular component, and molecular function. Meanwhile, KEGG enrichment found that with Human T-cell leukemia virus, one infection was the most highly enhanced pathway. Furthermore, the MET/PTEN/TGF-β pathway was identified as a significant pathway that had important biological functions, including cell migration and motility by PPI network analysis in SW1990 cells. Taken together, our study found that G-6 is a potential anti-pancreatic cancer agent with regulation of MET/PTEN/TGF-β pathway. |
| first_indexed | 2024-03-09T18:48:07Z |
| format | Article |
| id | doaj.art-95ea0449dc50467db43fbc5135f65fb9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:48:07Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-95ea0449dc50467db43fbc5135f65fb92023-11-24T06:05:05ZengMDPI AGMolecules1420-30492022-11-012721749010.3390/molecules27217490Potential Anti-Tumor Activity of Nardoguaianone L Isolated from <i>Nardostachys jatamansi</i> DC. in SW1990 CellsChun-Yan Sang0Yi-Dan Zheng1Li-Mei Ma2Kai Wang3Cheng-Bo Wang4Tian Chai5Komila A. Eshbakova6Jun-Li Yang7CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaCollege of Life Science, Northwest Normal University, Lanzhou 730070, ChinaCAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaCAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaCAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaCAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaS. Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences, Tashkent 100170, UzbekistanCAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, ChinaNatural products (NPs) were a rich source of diverse bioactive molecules. Most anti-tumor agents were built on natural scaffolds. <i>Nardostachys jatamansi</i> DC. was an important plant used to process the traditional Chinese herbal medicines “gansong”. Pancreatic cancer was the fourth most common cause of cancer-related death in the world. Hence, there was an urgent need to develop novel agents for the treatment of pancreatic cancer. In this paper, nardoguaianone L (G-6) is isolated from <i>N. jatamansi</i>, which inhibited SW1990 cells colony formation and cell migration, and induced cell apoptosis. Furthermore, we analyzed the differential expression proteins after treatment with G-6 in SW1990 cells by using iTRAQ/TMT-based quantitative proteomics technology, and the results showed that G-6 regulated 143 proteins’ differential expression by GO annotation, including biological process, cellular component, and molecular function. Meanwhile, KEGG enrichment found that with Human T-cell leukemia virus, one infection was the most highly enhanced pathway. Furthermore, the MET/PTEN/TGF-β pathway was identified as a significant pathway that had important biological functions, including cell migration and motility by PPI network analysis in SW1990 cells. Taken together, our study found that G-6 is a potential anti-pancreatic cancer agent with regulation of MET/PTEN/TGF-β pathway.https://www.mdpi.com/1420-3049/27/21/7490natural products<i>Nardostachys jatamansi</i> DC.pancreatic canceriTRAQ/TMTMET/PTEN/TGF-β pathway |
| spellingShingle | Chun-Yan Sang Yi-Dan Zheng Li-Mei Ma Kai Wang Cheng-Bo Wang Tian Chai Komila A. Eshbakova Jun-Li Yang Potential Anti-Tumor Activity of Nardoguaianone L Isolated from <i>Nardostachys jatamansi</i> DC. in SW1990 Cells Molecules natural products <i>Nardostachys jatamansi</i> DC. pancreatic cancer iTRAQ/TMT MET/PTEN/TGF-β pathway |
| title | Potential Anti-Tumor Activity of Nardoguaianone L Isolated from <i>Nardostachys jatamansi</i> DC. in SW1990 Cells |
| title_full | Potential Anti-Tumor Activity of Nardoguaianone L Isolated from <i>Nardostachys jatamansi</i> DC. in SW1990 Cells |
| title_fullStr | Potential Anti-Tumor Activity of Nardoguaianone L Isolated from <i>Nardostachys jatamansi</i> DC. in SW1990 Cells |
| title_full_unstemmed | Potential Anti-Tumor Activity of Nardoguaianone L Isolated from <i>Nardostachys jatamansi</i> DC. in SW1990 Cells |
| title_short | Potential Anti-Tumor Activity of Nardoguaianone L Isolated from <i>Nardostachys jatamansi</i> DC. in SW1990 Cells |
| title_sort | potential anti tumor activity of nardoguaianone l isolated from i nardostachys jatamansi i dc in sw1990 cells |
| topic | natural products <i>Nardostachys jatamansi</i> DC. pancreatic cancer iTRAQ/TMT MET/PTEN/TGF-β pathway |
| url | https://www.mdpi.com/1420-3049/27/21/7490 |
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