Direct deep UV lithography to micropattern PMMA for stem cell culture
Microengineering is increasingly being used for controlling the microenvironment of stem cells. Here, a novel method for fabricating structures with subcellular dimensions in commonly available thermoplastic poly(methyl methacrylate) (PMMA) is shown. Microstructures are produced in PMMA substrates u...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423002399 |
| _version_ | 1827815491518857216 |
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| author | Pinak Samal Jay Rabindra Kumar Samal Hoon Suk Rho Denis van Beurden Clemens van Blitterswijk Roman Truckenmüller Stefan Giselbrecht |
| author_facet | Pinak Samal Jay Rabindra Kumar Samal Hoon Suk Rho Denis van Beurden Clemens van Blitterswijk Roman Truckenmüller Stefan Giselbrecht |
| author_sort | Pinak Samal |
| collection | DOAJ |
| description | Microengineering is increasingly being used for controlling the microenvironment of stem cells. Here, a novel method for fabricating structures with subcellular dimensions in commonly available thermoplastic poly(methyl methacrylate) (PMMA) is shown. Microstructures are produced in PMMA substrates using Deep Ultraviolet lithography, and the effect of different developers is described. Microgrooves fabricated in PMMA are used for the neuronal differentiation of mouse embryonic stem cells (mESCs) directly on the polymer. The fabrication of 3D, curvilinear patterned surfaces is also highlighted. A 3D multilayered microfluidic chip is fabricated using this method, which includes a porous polycarbonate (PC) membrane as cell culture substrate. Besides directly manufacturing PMMA-based microfluidic devices, an application of the novel approach is shown where a reusable PMMA master is created for replicating microstructures with polydimethylsiloxane (PDMS). As an application example, microchannels fabricated in PDMS are used to selectively expose mESCs to soluble factors in a localized manner. The described microfabrication process offers a remarkably simple method to fabricate for example multifunctional topographical or microfluidic culture substrates outside cleanrooms, thereby using inexpensive and widely accessible equipment. The versatility of the underlying process could find various applications also in optical systems and surface modification of biomedical implants. |
| first_indexed | 2024-03-12T00:00:16Z |
| format | Article |
| id | doaj.art-95eab6a8cb11412b86ec98441e44bbaf |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-12T00:00:16Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-95eab6a8cb11412b86ec98441e44bbaf2023-09-18T04:30:22ZengElsevierMaterials Today Bio2590-00642023-10-0122100779Direct deep UV lithography to micropattern PMMA for stem cell culturePinak Samal0Jay Rabindra Kumar Samal1Hoon Suk Rho2Denis van Beurden3Clemens van Blitterswijk4Roman Truckenmüller5Stefan Giselbrecht6MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER, Maastricht, the NetherlandsMERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER, Maastricht, the NetherlandsMERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER, Maastricht, the Netherlands; Mepsgen Co., Ltd., 7F, Hanyang Tower, 12, Beobwon-ro-11-gil, Songpa-gu, Seoul, Republic of KoreaMERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER, Maastricht, the NetherlandsMERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER, Maastricht, the NetherlandsMERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER, Maastricht, the Netherlands; Corresponding author.MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER, Maastricht, the Netherlands; Corresponding author.Microengineering is increasingly being used for controlling the microenvironment of stem cells. Here, a novel method for fabricating structures with subcellular dimensions in commonly available thermoplastic poly(methyl methacrylate) (PMMA) is shown. Microstructures are produced in PMMA substrates using Deep Ultraviolet lithography, and the effect of different developers is described. Microgrooves fabricated in PMMA are used for the neuronal differentiation of mouse embryonic stem cells (mESCs) directly on the polymer. The fabrication of 3D, curvilinear patterned surfaces is also highlighted. A 3D multilayered microfluidic chip is fabricated using this method, which includes a porous polycarbonate (PC) membrane as cell culture substrate. Besides directly manufacturing PMMA-based microfluidic devices, an application of the novel approach is shown where a reusable PMMA master is created for replicating microstructures with polydimethylsiloxane (PDMS). As an application example, microchannels fabricated in PDMS are used to selectively expose mESCs to soluble factors in a localized manner. The described microfabrication process offers a remarkably simple method to fabricate for example multifunctional topographical or microfluidic culture substrates outside cleanrooms, thereby using inexpensive and widely accessible equipment. The versatility of the underlying process could find various applications also in optical systems and surface modification of biomedical implants.http://www.sciencedirect.com/science/article/pii/S2590006423002399Poly(methyl methacrylate) (PMMA)Deep UV lithographyMicrostructuresMicrofluidicsStem cell patterning |
| spellingShingle | Pinak Samal Jay Rabindra Kumar Samal Hoon Suk Rho Denis van Beurden Clemens van Blitterswijk Roman Truckenmüller Stefan Giselbrecht Direct deep UV lithography to micropattern PMMA for stem cell culture Materials Today Bio Poly(methyl methacrylate) (PMMA) Deep UV lithography Microstructures Microfluidics Stem cell patterning |
| title | Direct deep UV lithography to micropattern PMMA for stem cell culture |
| title_full | Direct deep UV lithography to micropattern PMMA for stem cell culture |
| title_fullStr | Direct deep UV lithography to micropattern PMMA for stem cell culture |
| title_full_unstemmed | Direct deep UV lithography to micropattern PMMA for stem cell culture |
| title_short | Direct deep UV lithography to micropattern PMMA for stem cell culture |
| title_sort | direct deep uv lithography to micropattern pmma for stem cell culture |
| topic | Poly(methyl methacrylate) (PMMA) Deep UV lithography Microstructures Microfluidics Stem cell patterning |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423002399 |
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