<i>In Vitro</i> Susceptibility of <i>Cryptosporidium parvum</i> to Plant Antiparasitic Compounds
<i>Cryptosporidium parvum</i> is a significant cause of watery diarrhoea in humans and other animals worldwide. Although hundreds of novel drugs have been evaluated, no effective specific chemotherapeutic intervention for <i>C. parvum</i> has been reported. There has been muc...
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MDPI AG
2022-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/1/61 |
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| author | Sandamalie Ranasinghe Alireza Zahedi Anthony Armson Alan J. Lymbery Amanda Ash |
| author_facet | Sandamalie Ranasinghe Alireza Zahedi Anthony Armson Alan J. Lymbery Amanda Ash |
| author_sort | Sandamalie Ranasinghe |
| collection | DOAJ |
| description | <i>Cryptosporidium parvum</i> is a significant cause of watery diarrhoea in humans and other animals worldwide. Although hundreds of novel drugs have been evaluated, no effective specific chemotherapeutic intervention for <i>C. parvum</i> has been reported. There has been much recent interest in evaluating plant-derived products in the fight against gastrointestinal parasites, including <i>C. parvum</i>. This study aimed to identify extracts from 13 different plant species that provide evidence for inhibiting the growth of <i>C. parvum</i> in vitro. Efficacy against <i>C. parvum</i> was detected and quantified using quantitative PCR and immunofluorescence assays. All plant extracts tested against <i>C. parvum</i> showed varying inhibition activities in vitro, and none of them produced a cytotoxic effect on HCT-8 cells at concentrations up to 500 µg/mL. Four plant species with the strongest evidence of activity against <i>C. parvum</i> were <i>Curcuma longa</i>, <i>Piper nigrum, Embelia ribes</i>, and <i>Nigella sativa,</i> all with dose-dependent efficacy. To the authors' knowledge, this is the first time that these plant extracts have proven to be experimentally efficacious against <i>C. parvum</i>. These results support further exploration of these plants and their compounds as possible treatments for <i>Cryptosporidium</i> infections. |
| first_indexed | 2024-03-09T11:30:43Z |
| format | Article |
| id | doaj.art-95ed77c102394a08bea6a87afa8f4d4c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T11:30:43Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-95ed77c102394a08bea6a87afa8f4d4c2023-11-30T23:53:12ZengMDPI AGPathogens2076-08172022-12-011216110.3390/pathogens12010061<i>In Vitro</i> Susceptibility of <i>Cryptosporidium parvum</i> to Plant Antiparasitic CompoundsSandamalie Ranasinghe0Alireza Zahedi1Anthony Armson2Alan J. Lymbery3Amanda Ash4Centre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Perth 6150, AustraliaCentre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Perth 6150, AustraliaPsychology, Counselling, Exercise Science and Chiropractic, College of Science, Health, Engineering and Education, Murdoch University, Perth 6150, AustraliaCentre for Sustainable Aquatic Ecosystems, Harry Butler Institute, Murdoch University, Perth 6150, AustraliaCentre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Perth 6150, Australia<i>Cryptosporidium parvum</i> is a significant cause of watery diarrhoea in humans and other animals worldwide. Although hundreds of novel drugs have been evaluated, no effective specific chemotherapeutic intervention for <i>C. parvum</i> has been reported. There has been much recent interest in evaluating plant-derived products in the fight against gastrointestinal parasites, including <i>C. parvum</i>. This study aimed to identify extracts from 13 different plant species that provide evidence for inhibiting the growth of <i>C. parvum</i> in vitro. Efficacy against <i>C. parvum</i> was detected and quantified using quantitative PCR and immunofluorescence assays. All plant extracts tested against <i>C. parvum</i> showed varying inhibition activities in vitro, and none of them produced a cytotoxic effect on HCT-8 cells at concentrations up to 500 µg/mL. Four plant species with the strongest evidence of activity against <i>C. parvum</i> were <i>Curcuma longa</i>, <i>Piper nigrum, Embelia ribes</i>, and <i>Nigella sativa,</i> all with dose-dependent efficacy. To the authors' knowledge, this is the first time that these plant extracts have proven to be experimentally efficacious against <i>C. parvum</i>. These results support further exploration of these plants and their compounds as possible treatments for <i>Cryptosporidium</i> infections.https://www.mdpi.com/2076-0817/12/1/61Ayurvedacytotoxicitycryptosporidiosisdrug screeningplant medicine |
| spellingShingle | Sandamalie Ranasinghe Alireza Zahedi Anthony Armson Alan J. Lymbery Amanda Ash <i>In Vitro</i> Susceptibility of <i>Cryptosporidium parvum</i> to Plant Antiparasitic Compounds Pathogens Ayurveda cytotoxicity cryptosporidiosis drug screening plant medicine |
| title | <i>In Vitro</i> Susceptibility of <i>Cryptosporidium parvum</i> to Plant Antiparasitic Compounds |
| title_full | <i>In Vitro</i> Susceptibility of <i>Cryptosporidium parvum</i> to Plant Antiparasitic Compounds |
| title_fullStr | <i>In Vitro</i> Susceptibility of <i>Cryptosporidium parvum</i> to Plant Antiparasitic Compounds |
| title_full_unstemmed | <i>In Vitro</i> Susceptibility of <i>Cryptosporidium parvum</i> to Plant Antiparasitic Compounds |
| title_short | <i>In Vitro</i> Susceptibility of <i>Cryptosporidium parvum</i> to Plant Antiparasitic Compounds |
| title_sort | i in vitro i susceptibility of i cryptosporidium parvum i to plant antiparasitic compounds |
| topic | Ayurveda cytotoxicity cryptosporidiosis drug screening plant medicine |
| url | https://www.mdpi.com/2076-0817/12/1/61 |
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