Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak
(1) Background: We determined the relevance of intestinal dominance by <i>Serratia</i> spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. <i>Serratia</i> spp. was cultured from swabs obtained from 13 neonates (Gr...
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MDPI AG
2021-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2271 |
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| author | Elias Dahdouh Fernando Lázaro-Perona Guillermo Ruiz-Carrascoso Laura Sánchez García Miguel Saenz de Pipaón Jesús Mingorance |
| author_facet | Elias Dahdouh Fernando Lázaro-Perona Guillermo Ruiz-Carrascoso Laura Sánchez García Miguel Saenz de Pipaón Jesús Mingorance |
| author_sort | Elias Dahdouh |
| collection | DOAJ |
| description | (1) Background: We determined the relevance of intestinal dominance by <i>Serratia</i> spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. <i>Serratia</i> spp. was cultured from swabs obtained from 13 neonates (Group 1), while the other 29 neonates were culture-negative (Group 2). Total DNA was extracted from rectal swabs, and quantitative PCRs (qPCRs) using <i>Serratia</i>- and <i>16SrRNA</i>-gene-specific primers were performed. relative intestinal loads (RLs) were determined using ΔΔC<sub>t</sub>. Clonality was investigated by random amplified polymorphic DNA analysis and whole-genome sequencing. (3) Results: The outbreak was caused by <i>Serratia marcescens</i> during the first eight weeks and <i>Serratia ureilytica</i> during the remaining five weeks. <i>Serratia</i> spp. were detected by qPCR in all Group 1 neonates and eleven Group 2 neonates. RLs of <i>Serratia</i> spp. were higher in Group 1 as compared to Group 2 (6.31% vs. 0.09%, <i>p</i> < 0.05) and in the first swab compared to the last (26.9% vs. 4.37%, <i>p</i> < 0.05). Nine neonates had extraintestinal detection of <i>Serratia</i> spp.; eight of them were infected. RLs of the patients with extraintestinal spread were higher than the rest (2.79% vs. 0.29%, <i>p</i> < 0.05). (4) Conclusions: Intestinal dominance by <i>Serratia</i> spp. plays a role in outbreaks and extraintestinal spread. |
| first_indexed | 2024-03-10T05:15:58Z |
| format | Article |
| id | doaj.art-95ee7026732240fcadeba90f269395e8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:15:58Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-95ee7026732240fcadeba90f269395e82023-11-23T00:28:54ZengMDPI AGMicroorganisms2076-26072021-10-01911227110.3390/microorganisms9112271Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an OutbreakElias Dahdouh0Fernando Lázaro-Perona1Guillermo Ruiz-Carrascoso2Laura Sánchez García3Miguel Saenz de Pipaón4Jesús Mingorance5Servicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046 Madrid, SpainServicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046 Madrid, SpainServicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046 Madrid, SpainServicio de Neonatología, Hospital Universitario La Paz, 28046 Madrid, SpainServicio de Neonatología, Hospital Universitario La Paz, 28046 Madrid, SpainServicio de Microbiología, Hospital Universitario La Paz, IdiPAZ, Paseo de la Castellana, 261, 28046 Madrid, Spain(1) Background: We determined the relevance of intestinal dominance by <i>Serratia</i> spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. <i>Serratia</i> spp. was cultured from swabs obtained from 13 neonates (Group 1), while the other 29 neonates were culture-negative (Group 2). Total DNA was extracted from rectal swabs, and quantitative PCRs (qPCRs) using <i>Serratia</i>- and <i>16SrRNA</i>-gene-specific primers were performed. relative intestinal loads (RLs) were determined using ΔΔC<sub>t</sub>. Clonality was investigated by random amplified polymorphic DNA analysis and whole-genome sequencing. (3) Results: The outbreak was caused by <i>Serratia marcescens</i> during the first eight weeks and <i>Serratia ureilytica</i> during the remaining five weeks. <i>Serratia</i> spp. were detected by qPCR in all Group 1 neonates and eleven Group 2 neonates. RLs of <i>Serratia</i> spp. were higher in Group 1 as compared to Group 2 (6.31% vs. 0.09%, <i>p</i> < 0.05) and in the first swab compared to the last (26.9% vs. 4.37%, <i>p</i> < 0.05). Nine neonates had extraintestinal detection of <i>Serratia</i> spp.; eight of them were infected. RLs of the patients with extraintestinal spread were higher than the rest (2.79% vs. 0.29%, <i>p</i> < 0.05). (4) Conclusions: Intestinal dominance by <i>Serratia</i> spp. plays a role in outbreaks and extraintestinal spread.https://www.mdpi.com/2076-2607/9/11/2271<i>Serratia marcescens</i><i>Serratia ureilytica</i>neonatal wardintestinal dominanceqPCR |
| spellingShingle | Elias Dahdouh Fernando Lázaro-Perona Guillermo Ruiz-Carrascoso Laura Sánchez García Miguel Saenz de Pipaón Jesús Mingorance Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak Microorganisms <i>Serratia marcescens</i> <i>Serratia ureilytica</i> neonatal ward intestinal dominance qPCR |
| title | Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak |
| title_full | Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak |
| title_fullStr | Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak |
| title_full_unstemmed | Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak |
| title_short | Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak |
| title_sort | intestinal dominance by i serratia marcescens i and i serratia ureilytica i among neonates in the setting of an outbreak |
| topic | <i>Serratia marcescens</i> <i>Serratia ureilytica</i> neonatal ward intestinal dominance qPCR |
| url | https://www.mdpi.com/2076-2607/9/11/2271 |
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