Identification of Aedes aegypti salivary gland proteins interacting with human immune receptor proteins.
Mosquito saliva proteins modulate the human immune and hemostatic systems and control mosquito-borne pathogenic infections. One mechanism through which mosquito proteins may influence host immunity and hemostasis is their interactions with key human receptor proteins that may act as receptors for or...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-09-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0010743 |
| _version_ | 1828329219299475456 |
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| author | Edem Gavor Yeu Khai Choong Yonghao Liu Julien Pompon Eng Eong Ooi Yu Keung Mok Haiyan Liu R Manjunatha Kini J Sivaraman |
| author_facet | Edem Gavor Yeu Khai Choong Yonghao Liu Julien Pompon Eng Eong Ooi Yu Keung Mok Haiyan Liu R Manjunatha Kini J Sivaraman |
| author_sort | Edem Gavor |
| collection | DOAJ |
| description | Mosquito saliva proteins modulate the human immune and hemostatic systems and control mosquito-borne pathogenic infections. One mechanism through which mosquito proteins may influence host immunity and hemostasis is their interactions with key human receptor proteins that may act as receptors for or coordinate attacks against invading pathogens. Here, using pull-down assays and proteomics-based mass spectrometry, we identified 11 Ae. aegypti salivary gland proteins (SGPs) (e.g., apyrase, Ae. aegypti venom allergen-1 [AaVA-1], neutrophil stimulating protein 1 [NeSt1], and D7 proteins), that interact with one or more of five human receptor proteins (cluster of differentiation 4 [CD4], CD14, CD86, dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin [DC-SIGN], and Toll-like receptor 4 [TLR4]). We focused on CD4- and DC-SIGN-interacting proteins and confirmed that CD4 directly interacts with AaVA-1, D7, and NeST1 recombinant proteins and that AaVA-1 showed a moderate interaction with DC-SIGN using ELISA. Bacteria responsive protein 1 (AgBR1), an Ae. aegypti saliva protein reported to enhance ZIKV infection in humans but that was not identified in our pull-down assay moderately interacts with CD4 in the ELISA assay. Functionally, we showed that AaVA-1 and NeST1 proteins promoted activation of CD4+ T cells. We propose the possible impact of these interactions and effects on mosquito-borne viral infections such as dengue, Zika, and chikungunya viruses. Overall, this study provides key insight into the vector-host (protein-protein) interaction network and suggests roles for these interactions in mosquito-borne viral infections. |
| first_indexed | 2024-04-13T20:21:18Z |
| format | Article |
| id | doaj.art-95f02715ee0e49d99342b9a0e1dd98e1 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-13T20:21:18Z |
| publishDate | 2022-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-95f02715ee0e49d99342b9a0e1dd98e12022-12-22T02:31:32ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352022-09-01169e001074310.1371/journal.pntd.0010743Identification of Aedes aegypti salivary gland proteins interacting with human immune receptor proteins.Edem GavorYeu Khai ChoongYonghao LiuJulien PomponEng Eong OoiYu Keung MokHaiyan LiuR Manjunatha KiniJ SivaramanMosquito saliva proteins modulate the human immune and hemostatic systems and control mosquito-borne pathogenic infections. One mechanism through which mosquito proteins may influence host immunity and hemostasis is their interactions with key human receptor proteins that may act as receptors for or coordinate attacks against invading pathogens. Here, using pull-down assays and proteomics-based mass spectrometry, we identified 11 Ae. aegypti salivary gland proteins (SGPs) (e.g., apyrase, Ae. aegypti venom allergen-1 [AaVA-1], neutrophil stimulating protein 1 [NeSt1], and D7 proteins), that interact with one or more of five human receptor proteins (cluster of differentiation 4 [CD4], CD14, CD86, dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin [DC-SIGN], and Toll-like receptor 4 [TLR4]). We focused on CD4- and DC-SIGN-interacting proteins and confirmed that CD4 directly interacts with AaVA-1, D7, and NeST1 recombinant proteins and that AaVA-1 showed a moderate interaction with DC-SIGN using ELISA. Bacteria responsive protein 1 (AgBR1), an Ae. aegypti saliva protein reported to enhance ZIKV infection in humans but that was not identified in our pull-down assay moderately interacts with CD4 in the ELISA assay. Functionally, we showed that AaVA-1 and NeST1 proteins promoted activation of CD4+ T cells. We propose the possible impact of these interactions and effects on mosquito-borne viral infections such as dengue, Zika, and chikungunya viruses. Overall, this study provides key insight into the vector-host (protein-protein) interaction network and suggests roles for these interactions in mosquito-borne viral infections.https://doi.org/10.1371/journal.pntd.0010743 |
| spellingShingle | Edem Gavor Yeu Khai Choong Yonghao Liu Julien Pompon Eng Eong Ooi Yu Keung Mok Haiyan Liu R Manjunatha Kini J Sivaraman Identification of Aedes aegypti salivary gland proteins interacting with human immune receptor proteins. PLoS Neglected Tropical Diseases |
| title | Identification of Aedes aegypti salivary gland proteins interacting with human immune receptor proteins. |
| title_full | Identification of Aedes aegypti salivary gland proteins interacting with human immune receptor proteins. |
| title_fullStr | Identification of Aedes aegypti salivary gland proteins interacting with human immune receptor proteins. |
| title_full_unstemmed | Identification of Aedes aegypti salivary gland proteins interacting with human immune receptor proteins. |
| title_short | Identification of Aedes aegypti salivary gland proteins interacting with human immune receptor proteins. |
| title_sort | identification of aedes aegypti salivary gland proteins interacting with human immune receptor proteins |
| url | https://doi.org/10.1371/journal.pntd.0010743 |
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