Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model
Summary: Exosomes mediate intracellular communication between cancer cells and the local/distant microenvironment, which promotes systemic dissemination of cancer. Here, we present a protocol for tumor-derived exosome isolation and in vivo metastasis evaluation in a mouse model. We describe steps fo...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004112 |
| _version_ | 1797781867367235584 |
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| author | Yu Jin Lee Kyeong Jin Shin Young Chan Chae |
| author_facet | Yu Jin Lee Kyeong Jin Shin Young Chan Chae |
| author_sort | Yu Jin Lee |
| collection | DOAJ |
| description | Summary: Exosomes mediate intracellular communication between cancer cells and the local/distant microenvironment, which promotes systemic dissemination of cancer. Here, we present a protocol for tumor-derived exosome isolation and in vivo metastasis evaluation in a mouse model. We describe steps for isolating and characterizing exosomes, establishing a metastatic mouse model, and injecting exosomes into mouse. We then detail hematoxylin and eosin staining and analysis. This protocol can be used to investigate exosome function and identify unexplored metastatic regulators related to exosome biogenesis.For complete details on the use and execution of this protocol, please refer to Lee et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-13T00:02:58Z |
| format | Article |
| id | doaj.art-95f05d24e1a1473ea066d706f143ea3c |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-13T00:02:58Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-95f05d24e1a1473ea066d706f143ea3c2023-07-13T05:30:49ZengElsevierSTAR Protocols2666-16672023-09-0143102444Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse modelYu Jin Lee0Kyeong Jin Shin1Young Chan Chae2Department of Biological Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of KoreaDepartment of Biological Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of KoreaDepartment of Biological Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea; Corresponding authorSummary: Exosomes mediate intracellular communication between cancer cells and the local/distant microenvironment, which promotes systemic dissemination of cancer. Here, we present a protocol for tumor-derived exosome isolation and in vivo metastasis evaluation in a mouse model. We describe steps for isolating and characterizing exosomes, establishing a metastatic mouse model, and injecting exosomes into mouse. We then detail hematoxylin and eosin staining and analysis. This protocol can be used to investigate exosome function and identify unexplored metastatic regulators related to exosome biogenesis.For complete details on the use and execution of this protocol, please refer to Lee et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723004112Cell BiologyCancerModel Organisms |
| spellingShingle | Yu Jin Lee Kyeong Jin Shin Young Chan Chae Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model STAR Protocols Cell Biology Cancer Model Organisms |
| title | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
| title_full | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
| title_fullStr | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
| title_full_unstemmed | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
| title_short | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
| title_sort | protocol for evaluation of tumor derived exosome induced cancer cell metastasis in a mouse model |
| topic | Cell Biology Cancer Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723004112 |
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