Protective Effects of Hydro-alcoholic Extract of Apium Graveolens on Carbon Tetrachloride Induced Hepatotoxicity in Sprague-Dawley male Rats
Background & Objective: Apium graveolens has antioxidant property because of its flavonoid compounds. In the present study, we investigated the hepatoprotective effects of hydroalcoholic extract of Apium graveolens in Sprague Dawley male rats treated with carbon tetrachloride (CCl4).Materials &a...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Fasa University of Medical Sciences
2015-05-01
|
| Series: | Journal of Fasa University of Medical Sciences |
| Subjects: | |
| Online Access: | http://journal.fums.ac.ir/browse.php?a_code=A-10-673-2&slc_lang=en&sid=1 |
| _version_ | 1818023145510010880 |
|---|---|
| author | Zahra Talebanpour bayat Heydar Aqababa Manzar Banoo Shojaiefard |
| author_facet | Zahra Talebanpour bayat Heydar Aqababa Manzar Banoo Shojaiefard |
| author_sort | Zahra Talebanpour bayat |
| collection | DOAJ |
| description | Background & Objective: Apium
graveolens has antioxidant property because of its flavonoid compounds. In the
present study, we investigated the hepatoprotective effects of hydroalcoholic
extract of Apium graveolens in Sprague Dawley male rats treated with
carbon tetrachloride (CCl4).Materials & Methods: In
this study, 48 rats (weighted 180-200 gram) were divided into six groups each
having eight rats. The six groups were control, sham, CCl4, and
three experimental groups. The experimental groups (4-6) received 1ml/kg CCl4
twice a week and olive oil with the ratio of 1:1 by intraperitoneal injection
for 40 days. In addition, they received 2 cc hydroalcoholic extract of Apium
graveolens by daily gavage with the concentrations of 200, 400, and 600
mg/kg/cc, respectively. The sham group received only olive oil with the
mentioned ratio and the other group received CCl4 and olive
oil. At the end, Alkaline Phosphatase
(ALP), Aspartate amino Transferase (AST), Alanine amino Transferase (ALT), and
the serum concentrations of albumin, total and direct bilirubin were measured
and analyzed by using statistical ANOVA Test. Results: Injection of CCl4
increased the serum levels of liver enzymes and total and direct bilirubin but
it decreased the concentration of serum albumin. On the other hand,
hydroalcoholic extract of Apium graveolens decreased the liver enzymes and
increased albumin level (p<0.001). Conclusion: The
results of this study reveals that the consumption of hydro-alcoholic extract
of Apium graveolens maintain the integrity of the liver and protects it against
damage. |
| first_indexed | 2024-12-10T03:39:40Z |
| format | Article |
| id | doaj.art-95f275000c7e447a920bff3ad3063479 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2228-7329 |
| language | fas |
| last_indexed | 2024-12-10T03:39:40Z |
| publishDate | 2015-05-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Fasa University of Medical Sciences |
| spelling | doaj.art-95f275000c7e447a920bff3ad30634792022-12-22T02:03:36ZfasFasa University of Medical SciencesJournal of Fasa University of Medical Sciences2228-51052228-73292015-05-0151102110Protective Effects of Hydro-alcoholic Extract of Apium Graveolens on Carbon Tetrachloride Induced Hepatotoxicity in Sprague-Dawley male RatsZahra Talebanpour bayat0Heydar Aqababa1Manzar Banoo Shojaiefard2 Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran. Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran. Department of physiology, Fasa University of Medical Sciences, Fasa, Iran. Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran. Background & Objective: Apium graveolens has antioxidant property because of its flavonoid compounds. In the present study, we investigated the hepatoprotective effects of hydroalcoholic extract of Apium graveolens in Sprague Dawley male rats treated with carbon tetrachloride (CCl4).Materials & Methods: In this study, 48 rats (weighted 180-200 gram) were divided into six groups each having eight rats. The six groups were control, sham, CCl4, and three experimental groups. The experimental groups (4-6) received 1ml/kg CCl4 twice a week and olive oil with the ratio of 1:1 by intraperitoneal injection for 40 days. In addition, they received 2 cc hydroalcoholic extract of Apium graveolens by daily gavage with the concentrations of 200, 400, and 600 mg/kg/cc, respectively. The sham group received only olive oil with the mentioned ratio and the other group received CCl4 and olive oil. At the end, Alkaline Phosphatase (ALP), Aspartate amino Transferase (AST), Alanine amino Transferase (ALT), and the serum concentrations of albumin, total and direct bilirubin were measured and analyzed by using statistical ANOVA Test. Results: Injection of CCl4 increased the serum levels of liver enzymes and total and direct bilirubin but it decreased the concentration of serum albumin. On the other hand, hydroalcoholic extract of Apium graveolens decreased the liver enzymes and increased albumin level (p<0.001). Conclusion: The results of this study reveals that the consumption of hydro-alcoholic extract of Apium graveolens maintain the integrity of the liver and protects it against damage.http://journal.fums.ac.ir/browse.php?a_code=A-10-673-2&slc_lang=en&sid=1Apium graveolens CCl4 hepatic protection rat |
| spellingShingle | Zahra Talebanpour bayat Heydar Aqababa Manzar Banoo Shojaiefard Protective Effects of Hydro-alcoholic Extract of Apium Graveolens on Carbon Tetrachloride Induced Hepatotoxicity in Sprague-Dawley male Rats Journal of Fasa University of Medical Sciences Apium graveolens CCl4 hepatic protection rat |
| title | Protective Effects of Hydro-alcoholic Extract of Apium Graveolens on Carbon Tetrachloride Induced Hepatotoxicity in Sprague-Dawley male Rats |
| title_full | Protective Effects of Hydro-alcoholic Extract of Apium Graveolens on Carbon Tetrachloride Induced Hepatotoxicity in Sprague-Dawley male Rats |
| title_fullStr | Protective Effects of Hydro-alcoholic Extract of Apium Graveolens on Carbon Tetrachloride Induced Hepatotoxicity in Sprague-Dawley male Rats |
| title_full_unstemmed | Protective Effects of Hydro-alcoholic Extract of Apium Graveolens on Carbon Tetrachloride Induced Hepatotoxicity in Sprague-Dawley male Rats |
| title_short | Protective Effects of Hydro-alcoholic Extract of Apium Graveolens on Carbon Tetrachloride Induced Hepatotoxicity in Sprague-Dawley male Rats |
| title_sort | protective effects of hydro alcoholic extract of apium graveolens on carbon tetrachloride induced hepatotoxicity in sprague dawley male rats |
| topic | Apium graveolens CCl4 hepatic protection rat |
| url | http://journal.fums.ac.ir/browse.php?a_code=A-10-673-2&slc_lang=en&sid=1 |
| work_keys_str_mv | AT zahratalebanpourbayat protectiveeffectsofhydroalcoholicextractofapiumgraveolensoncarbontetrachlorideinducedhepatotoxicityinspraguedawleymalerats AT heydaraqababa protectiveeffectsofhydroalcoholicextractofapiumgraveolensoncarbontetrachlorideinducedhepatotoxicityinspraguedawleymalerats AT manzarbanooshojaiefard protectiveeffectsofhydroalcoholicextractofapiumgraveolensoncarbontetrachlorideinducedhepatotoxicityinspraguedawleymalerats |