Improvement of mechanical properties of collagen electrospun mats by halloysite nanotubes
Collagen electrospun fibers had emerged as a promising scaffold for tissue engineering applications, nonetheless, pristine collagen fibers fail to provide of adequate mechanical properties. Therefore, here we propose the addition of halloysite nanotubes (HNT) into collagen solution for the obtention...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Materials Research and Technology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2238785422011796 |
| _version_ | 1811253074852839424 |
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| author | A. Hernández Rangel R.G. Casañas Pimentel E. San Martin Martinez |
| author_facet | A. Hernández Rangel R.G. Casañas Pimentel E. San Martin Martinez |
| author_sort | A. Hernández Rangel |
| collection | DOAJ |
| description | Collagen electrospun fibers had emerged as a promising scaffold for tissue engineering applications, nonetheless, pristine collagen fibers fail to provide of adequate mechanical properties. Therefore, here we propose the addition of halloysite nanotubes (HNT) into collagen solution for the obtention of nanofibrous mats with improved mechanical performance. Collagen was isolated and purified from tilapia skin and different concentrations of HNT (0.5, 1.0, and 2.0 %wt) were added to further spin the collagen-HNT solutions. HNT incorporation augmented the elongation at break in 800% but not in a linear manner, the smallest concentration of HNT used was the one with the better results, probably due to the agglomeration of HNT at higher concentrations as shown by SEM micrographs. Finally, the human dermal fibroblast (HDF) cell viability assay demonstrated that COL-HNT membranes were biocompatible up to a concentration of less than 1.0% and that concentrations greater than 2.0% significantly affect membrane permeability, subsequently leading to the death of the cells. Our results show that HNT can be incorporated into collagen to obtain nanofiber scaffolds, with improved mechanical properties up to 0.5% of HNT, being important in the field of tissue engineering. |
| first_indexed | 2024-04-12T16:45:21Z |
| format | Article |
| id | doaj.art-95f3a44675d548fea03ba7eea7c71f5e |
| institution | Directory Open Access Journal |
| issn | 2238-7854 |
| language | English |
| last_indexed | 2024-04-12T16:45:21Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Materials Research and Technology |
| spelling | doaj.art-95f3a44675d548fea03ba7eea7c71f5e2022-12-22T03:24:37ZengElsevierJournal of Materials Research and Technology2238-78542022-09-012035923599Improvement of mechanical properties of collagen electrospun mats by halloysite nanotubesA. Hernández Rangel0R.G. Casañas Pimentel1E. San Martin Martinez2Instituto Politécnico Nacional, Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, C.P. 11500 Ciudad de México, MexicoInstituto Politécnico Nacional, Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, C.P. 11500 Ciudad de México, MexicoCorresponding author.; Instituto Politécnico Nacional, Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, C.P. 11500 Ciudad de México, MexicoCollagen electrospun fibers had emerged as a promising scaffold for tissue engineering applications, nonetheless, pristine collagen fibers fail to provide of adequate mechanical properties. Therefore, here we propose the addition of halloysite nanotubes (HNT) into collagen solution for the obtention of nanofibrous mats with improved mechanical performance. Collagen was isolated and purified from tilapia skin and different concentrations of HNT (0.5, 1.0, and 2.0 %wt) were added to further spin the collagen-HNT solutions. HNT incorporation augmented the elongation at break in 800% but not in a linear manner, the smallest concentration of HNT used was the one with the better results, probably due to the agglomeration of HNT at higher concentrations as shown by SEM micrographs. Finally, the human dermal fibroblast (HDF) cell viability assay demonstrated that COL-HNT membranes were biocompatible up to a concentration of less than 1.0% and that concentrations greater than 2.0% significantly affect membrane permeability, subsequently leading to the death of the cells. Our results show that HNT can be incorporated into collagen to obtain nanofiber scaffolds, with improved mechanical properties up to 0.5% of HNT, being important in the field of tissue engineering.http://www.sciencedirect.com/science/article/pii/S2238785422011796Collagen electrospinningNanocomposite matsMechanical propertiesHalloysite nanotubes |
| spellingShingle | A. Hernández Rangel R.G. Casañas Pimentel E. San Martin Martinez Improvement of mechanical properties of collagen electrospun mats by halloysite nanotubes Journal of Materials Research and Technology Collagen electrospinning Nanocomposite mats Mechanical properties Halloysite nanotubes |
| title | Improvement of mechanical properties of collagen electrospun mats by halloysite nanotubes |
| title_full | Improvement of mechanical properties of collagen electrospun mats by halloysite nanotubes |
| title_fullStr | Improvement of mechanical properties of collagen electrospun mats by halloysite nanotubes |
| title_full_unstemmed | Improvement of mechanical properties of collagen electrospun mats by halloysite nanotubes |
| title_short | Improvement of mechanical properties of collagen electrospun mats by halloysite nanotubes |
| title_sort | improvement of mechanical properties of collagen electrospun mats by halloysite nanotubes |
| topic | Collagen electrospinning Nanocomposite mats Mechanical properties Halloysite nanotubes |
| url | http://www.sciencedirect.com/science/article/pii/S2238785422011796 |
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