Caproate production from Enset fiber in one-pot two-step fermentation using anaerobic fungi (Neocallimastix cameroonii strain G341) and Clostridium kluyveri DSM 555
Abstract Background Lignocellulosic biomass plays a crucial role in creating a circular bioeconomy and minimizing environmental impact. Enset biomass is a byproduct of traditional Ethiopian Enset food processing that is thrown away in huge quantities. This study aimed to produce caproate from Enset...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-10-01
|
| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02224-w |
| _version_ | 1827632648265138176 |
|---|---|
| author | Nebyat Seid Katrin Ochsenreither Anke Neumann |
| author_facet | Nebyat Seid Katrin Ochsenreither Anke Neumann |
| author_sort | Nebyat Seid |
| collection | DOAJ |
| description | Abstract Background Lignocellulosic biomass plays a crucial role in creating a circular bioeconomy and minimizing environmental impact. Enset biomass is a byproduct of traditional Ethiopian Enset food processing that is thrown away in huge quantities. This study aimed to produce caproate from Enset fiber using Neocallimastix cameroonii strain G341 and Clostridium kluyveri DSM 555 in one-pot two-step fermentation. Results The process started by growing N. cameroonii on Enset fiber as a carbon source for 7 days. Subsequently, the fungal culture was inoculated with active C. kluyveri preculture and further incubated. The results showed that N. cameroonii grew on 0.25 g untreated Enset fiber as the sole carbon source and produced 1.16 mmol acetate, 0.51 mmol hydrogen, and 1.34 mmol formate. In addition, lactate, succinate, and ethanol were detected in small amounts, 0.17 mmol, 0.08 mmol, and 0.7 mmol, respectively. After inoculating with C. kluyveri, 0.3 mmol of caproate and 0.48 mmol of butyrate were produced, and hydrogen production also increased to 0.95 mmol compared to sole N. cameroonii fermentation. Moreover, after the culture was supplemented with 2.18 mmol of ethanol during C. kluyveri inoculation, caproate, and hydrogen production was further increased to 1.2 and 1.36 mmol, respectively, and the consumption of acetate also increased. Conclusion A novel microbial cell factory was developed to convert untreated lignocellulosic Enset fiber into the medium chain carboxylic acid caproate and H2 by a co-culture of the anaerobic fungi N. cameroonii and C. kluyveri. This opens a new value chain for Enset farmers, as the process requires only locally available raw materials and low-price fermenters. As the caproate production was mainly limited by the available ethanol, the addition of locally produced ethanol-containing fermentation broth (“beer”) would further increase the titer. Graphical Abstract |
| first_indexed | 2024-03-09T14:48:30Z |
| format | Article |
| id | doaj.art-95f3d29c17244de2b9fe7d785823abd9 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-09T14:48:30Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-95f3d29c17244de2b9fe7d785823abd92023-11-26T14:37:50ZengBMCMicrobial Cell Factories1475-28592023-10-0122111210.1186/s12934-023-02224-wCaproate production from Enset fiber in one-pot two-step fermentation using anaerobic fungi (Neocallimastix cameroonii strain G341) and Clostridium kluyveri DSM 555Nebyat Seid0Katrin Ochsenreither1Anke Neumann2Electrobiotechnology, Institute of Process Engineering in Life Science 2, Karlsruhe Institute of Technology (KIT)Department of Chemical and Process Engineering, Karlsruhe Institute of Technology (KIT)Electrobiotechnology, Institute of Process Engineering in Life Science 2, Karlsruhe Institute of Technology (KIT)Abstract Background Lignocellulosic biomass plays a crucial role in creating a circular bioeconomy and minimizing environmental impact. Enset biomass is a byproduct of traditional Ethiopian Enset food processing that is thrown away in huge quantities. This study aimed to produce caproate from Enset fiber using Neocallimastix cameroonii strain G341 and Clostridium kluyveri DSM 555 in one-pot two-step fermentation. Results The process started by growing N. cameroonii on Enset fiber as a carbon source for 7 days. Subsequently, the fungal culture was inoculated with active C. kluyveri preculture and further incubated. The results showed that N. cameroonii grew on 0.25 g untreated Enset fiber as the sole carbon source and produced 1.16 mmol acetate, 0.51 mmol hydrogen, and 1.34 mmol formate. In addition, lactate, succinate, and ethanol were detected in small amounts, 0.17 mmol, 0.08 mmol, and 0.7 mmol, respectively. After inoculating with C. kluyveri, 0.3 mmol of caproate and 0.48 mmol of butyrate were produced, and hydrogen production also increased to 0.95 mmol compared to sole N. cameroonii fermentation. Moreover, after the culture was supplemented with 2.18 mmol of ethanol during C. kluyveri inoculation, caproate, and hydrogen production was further increased to 1.2 and 1.36 mmol, respectively, and the consumption of acetate also increased. Conclusion A novel microbial cell factory was developed to convert untreated lignocellulosic Enset fiber into the medium chain carboxylic acid caproate and H2 by a co-culture of the anaerobic fungi N. cameroonii and C. kluyveri. This opens a new value chain for Enset farmers, as the process requires only locally available raw materials and low-price fermenters. As the caproate production was mainly limited by the available ethanol, the addition of locally produced ethanol-containing fermentation broth (“beer”) would further increase the titer. Graphical Abstracthttps://doi.org/10.1186/s12934-023-02224-wEnset fiberOne-pot two-step fermentationCaproateN. cameroonii strain G341C. KluyveriAnaerobic fungi |
| spellingShingle | Nebyat Seid Katrin Ochsenreither Anke Neumann Caproate production from Enset fiber in one-pot two-step fermentation using anaerobic fungi (Neocallimastix cameroonii strain G341) and Clostridium kluyveri DSM 555 Microbial Cell Factories Enset fiber One-pot two-step fermentation Caproate N. cameroonii strain G341 C. Kluyveri Anaerobic fungi |
| title | Caproate production from Enset fiber in one-pot two-step fermentation using anaerobic fungi (Neocallimastix cameroonii strain G341) and Clostridium kluyveri DSM 555 |
| title_full | Caproate production from Enset fiber in one-pot two-step fermentation using anaerobic fungi (Neocallimastix cameroonii strain G341) and Clostridium kluyveri DSM 555 |
| title_fullStr | Caproate production from Enset fiber in one-pot two-step fermentation using anaerobic fungi (Neocallimastix cameroonii strain G341) and Clostridium kluyveri DSM 555 |
| title_full_unstemmed | Caproate production from Enset fiber in one-pot two-step fermentation using anaerobic fungi (Neocallimastix cameroonii strain G341) and Clostridium kluyveri DSM 555 |
| title_short | Caproate production from Enset fiber in one-pot two-step fermentation using anaerobic fungi (Neocallimastix cameroonii strain G341) and Clostridium kluyveri DSM 555 |
| title_sort | caproate production from enset fiber in one pot two step fermentation using anaerobic fungi neocallimastix cameroonii strain g341 and clostridium kluyveri dsm 555 |
| topic | Enset fiber One-pot two-step fermentation Caproate N. cameroonii strain G341 C. Kluyveri Anaerobic fungi |
| url | https://doi.org/10.1186/s12934-023-02224-w |
| work_keys_str_mv | AT nebyatseid caproateproductionfromensetfiberinonepottwostepfermentationusinganaerobicfungineocallimastixcamerooniistraing341andclostridiumkluyveridsm555 AT katrinochsenreither caproateproductionfromensetfiberinonepottwostepfermentationusinganaerobicfungineocallimastixcamerooniistraing341andclostridiumkluyveridsm555 AT ankeneumann caproateproductionfromensetfiberinonepottwostepfermentationusinganaerobicfungineocallimastixcamerooniistraing341andclostridiumkluyveridsm555 |