Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and Cattle
We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2003-02-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/9/2/02-0244_article |
| _version_ | 1818029543491895296 |
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| author | Giliane de Souza Trindade Flávio Guimarães da Fonseca João Trindade Marques Maurício Lacerda Nogueira Luiz Claudio Nogueira Mendes Alexandre Secorun Borges Juliana Regina Peiró Edviges Maristela Pituco Cláudio Antônio Bonjardim Paulo César Peregrino Ferreira Maurício Teixeira Lima |
| author_facet | Giliane de Souza Trindade Flávio Guimarães da Fonseca João Trindade Marques Maurício Lacerda Nogueira Luiz Claudio Nogueira Mendes Alexandre Secorun Borges Juliana Regina Peiró Edviges Maristela Pituco Cláudio Antônio Bonjardim Paulo César Peregrino Ferreira Maurício Teixeira Lima |
| author_sort | Giliane de Souza Trindade |
| collection | DOAJ |
| description | We describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus. |
| first_indexed | 2024-12-10T05:21:22Z |
| format | Article |
| id | doaj.art-95f52202ad92400483d4861d544fee03 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-10T05:21:22Z |
| publishDate | 2003-02-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-95f52202ad92400483d4861d544fee032022-12-22T02:00:47ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592003-02-019215516010.3201/eid0902.020244Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and CattleGiliane de Souza TrindadeFlávio Guimarães da FonsecaJoão Trindade MarquesMaurício Lacerda NogueiraLuiz Claudio Nogueira MendesAlexandre Secorun BorgesJuliana Regina PeiróEdviges Maristela PitucoCláudio Antônio BonjardimPaulo César Peregrino FerreiraMaurício Teixeira LimaWe describe a vaccinialike virus, Araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. Diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. Molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and DNA sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (VGF), thymidine kinase (TK), and hemagglutinin. We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. Araçatuba virus could be a novel vaccinialike virus or could represent the spread of Cantagalo virus.https://wwwnc.cdc.gov/eid/article/9/2/02-0244_articleAraçatuba viruscowpoxlike outbreakemergent orthopoxvirusTKVGFHA |
| spellingShingle | Giliane de Souza Trindade Flávio Guimarães da Fonseca João Trindade Marques Maurício Lacerda Nogueira Luiz Claudio Nogueira Mendes Alexandre Secorun Borges Juliana Regina Peiró Edviges Maristela Pituco Cláudio Antônio Bonjardim Paulo César Peregrino Ferreira Maurício Teixeira Lima Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and Cattle Emerging Infectious Diseases Araçatuba virus cowpoxlike outbreak emergent orthopoxvirus TK VGF HA |
| title | Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and Cattle |
| title_full | Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and Cattle |
| title_fullStr | Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and Cattle |
| title_full_unstemmed | Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and Cattle |
| title_short | Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and Cattle |
| title_sort | aracatuba virus a vaccinialike virus associated with infection in humans and cattle |
| topic | Araçatuba virus cowpoxlike outbreak emergent orthopoxvirus TK VGF HA |
| url | https://wwwnc.cdc.gov/eid/article/9/2/02-0244_article |
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