Topographic Cues Impact on Embryonic Stem Cell Zscan4-Metastate
The extracellular microenvironment proved to exert a potent regulatory effect over different aspects of Embryonic Stem Cells (ESCs) behavior. In particular, the employment of engineered culture surfaces aimed at modulating ESC self-organization resulted effective in directing ESCs toward specific fa...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00178/full |
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| author | Carlo F. Natale Tiziana Angrisano Luigi Pistelli Geppino Falco Viola Calabrò Paolo A. Netti Paolo A. Netti Paolo A. Netti Maurizio Ventre Maurizio Ventre Maurizio Ventre |
| author_facet | Carlo F. Natale Tiziana Angrisano Luigi Pistelli Geppino Falco Viola Calabrò Paolo A. Netti Paolo A. Netti Paolo A. Netti Maurizio Ventre Maurizio Ventre Maurizio Ventre |
| author_sort | Carlo F. Natale |
| collection | DOAJ |
| description | The extracellular microenvironment proved to exert a potent regulatory effect over different aspects of Embryonic Stem Cells (ESCs) behavior. In particular, the employment of engineered culture surfaces aimed at modulating ESC self-organization resulted effective in directing ESCs toward specific fate decision. ESCs fluctuate among different levels of functional potency and in this context the Zscan4 gene marks the so-called “metastate,” a cellular state in which ESCs retain both self-renewal and pluripotency capabilities. Here we investigated the impact of topographic cues on ESCs pluripotency, differentiation and organization capabilities. To this aim, we engineered culturing platforms of nanograted surfaces with different features size and we investigated their impact on ESCs multicellular organization and Zscan4 gene expression. We showed that the morphology of ESC-derived aggregates and Zscan4 expression are strictly intertwined. Our data suggest that ESC Zscan4 metastate can be promoted if the adhesive surface conditions guide cellular self-aggregation into 3D dome-like structure, in which both cell-material interactions and cell-cell contact are supportive for Zscan4 expression. |
| first_indexed | 2024-12-14T00:38:47Z |
| format | Article |
| id | doaj.art-95f6f0ac15f54f6f959eac289583510c |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-14T00:38:47Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-95f6f0ac15f54f6f959eac289583510c2022-12-21T23:24:29ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-03-01810.3389/fbioe.2020.00178514933Topographic Cues Impact on Embryonic Stem Cell Zscan4-MetastateCarlo F. Natale0Tiziana Angrisano1Luigi Pistelli2Geppino Falco3Viola Calabrò4Paolo A. Netti5Paolo A. Netti6Paolo A. Netti7Maurizio Ventre8Maurizio Ventre9Maurizio Ventre10Interdisciplinary Research Centre on Biomaterials, University of Naples Federico II, Naples, ItalyDepartment of Biology, University of Naples Federico II, Naples, ItalyDepartment of Biology, University of Naples Federico II, Naples, ItalyDepartment of Biology, University of Naples Federico II, Naples, ItalyDepartment of Biology, University of Naples Federico II, Naples, ItalyInterdisciplinary Research Centre on Biomaterials, University of Naples Federico II, Naples, ItalyDepartment of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Naples, ItalyCenter for Advanced Biomaterials for HealthCare@CRIB, Istituto Italiano di Tecnologia, Naples, ItalyInterdisciplinary Research Centre on Biomaterials, University of Naples Federico II, Naples, ItalyDepartment of Chemical, Materials and Industrial Production Engineering, University of Naples Federico II, Naples, ItalyCenter for Advanced Biomaterials for HealthCare@CRIB, Istituto Italiano di Tecnologia, Naples, ItalyThe extracellular microenvironment proved to exert a potent regulatory effect over different aspects of Embryonic Stem Cells (ESCs) behavior. In particular, the employment of engineered culture surfaces aimed at modulating ESC self-organization resulted effective in directing ESCs toward specific fate decision. ESCs fluctuate among different levels of functional potency and in this context the Zscan4 gene marks the so-called “metastate,” a cellular state in which ESCs retain both self-renewal and pluripotency capabilities. Here we investigated the impact of topographic cues on ESCs pluripotency, differentiation and organization capabilities. To this aim, we engineered culturing platforms of nanograted surfaces with different features size and we investigated their impact on ESCs multicellular organization and Zscan4 gene expression. We showed that the morphology of ESC-derived aggregates and Zscan4 expression are strictly intertwined. Our data suggest that ESC Zscan4 metastate can be promoted if the adhesive surface conditions guide cellular self-aggregation into 3D dome-like structure, in which both cell-material interactions and cell-cell contact are supportive for Zscan4 expression.https://www.frontiersin.org/article/10.3389/fbioe.2020.00178/fullembryonic stem cellZscan4topographycell adhesioncytoskeleton |
| spellingShingle | Carlo F. Natale Tiziana Angrisano Luigi Pistelli Geppino Falco Viola Calabrò Paolo A. Netti Paolo A. Netti Paolo A. Netti Maurizio Ventre Maurizio Ventre Maurizio Ventre Topographic Cues Impact on Embryonic Stem Cell Zscan4-Metastate Frontiers in Bioengineering and Biotechnology embryonic stem cell Zscan4 topography cell adhesion cytoskeleton |
| title | Topographic Cues Impact on Embryonic Stem Cell Zscan4-Metastate |
| title_full | Topographic Cues Impact on Embryonic Stem Cell Zscan4-Metastate |
| title_fullStr | Topographic Cues Impact on Embryonic Stem Cell Zscan4-Metastate |
| title_full_unstemmed | Topographic Cues Impact on Embryonic Stem Cell Zscan4-Metastate |
| title_short | Topographic Cues Impact on Embryonic Stem Cell Zscan4-Metastate |
| title_sort | topographic cues impact on embryonic stem cell zscan4 metastate |
| topic | embryonic stem cell Zscan4 topography cell adhesion cytoskeleton |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00178/full |
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