Metabolic Engineering of Extremophilic Bacterium <i>Deinococcus radiodurans</i> for the Production of the Novel Carotenoid Deinoxanthin
Deinoxanthin, a xanthophyll derived from <i>Deinococcus</i> species, is a unique organic compound that provides greater antioxidant effects compared to other carotenoids due to its superior scavenging activity against singlet oxygen and hydrogen peroxide. Therefore, it has attracted sign...
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MDPI AG
2020-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/1/44 |
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| author | Sun-Wook Jeong Jun-Ho Kim Ji-Woong Kim Chae Yeon Kim Su Young Kim Yong Jun Choi |
| author_facet | Sun-Wook Jeong Jun-Ho Kim Ji-Woong Kim Chae Yeon Kim Su Young Kim Yong Jun Choi |
| author_sort | Sun-Wook Jeong |
| collection | DOAJ |
| description | Deinoxanthin, a xanthophyll derived from <i>Deinococcus</i> species, is a unique organic compound that provides greater antioxidant effects compared to other carotenoids due to its superior scavenging activity against singlet oxygen and hydrogen peroxide. Therefore, it has attracted significant attention as a next-generation organic compound that has great potential as a natural ingredient in a food supplements. Although the microbial identification of deinoxanthin has been identified, mass production has not yet been achieved. Here, we report, for the first time, the development of an engineered extremophilic microorganism, <i>Deinococcus radiodurans</i> strain R1, that is capable of producing deinoxanthin through rational metabolic engineering and process optimization. The genes <i>crtB</i> and <i>dxs</i> were first introduced into the genome to reinforce the metabolic flux towards deinoxanthin. The optimal temperature was then identified through a comparative analysis of the mRNA expression of the two genes, while the carbon source was further optimized to increase deinoxanthin production. The final engineered <i>D. radiodurans</i> strain R1 was able to produce 394 ± 17.6 mg/L (102 ± 11.1 mg/g DCW) of deinoxanthin with a yield of 40.4 ± 1.2 mg/g sucrose and a productivity of 8.4 ± 0.2 mg/L/h from 10 g/L of sucrose. The final engineered strain and the strategies developed in the present study can act as the foundation for the industrial application of extremophilic microorganisms. |
| first_indexed | 2024-03-10T13:45:42Z |
| format | Article |
| id | doaj.art-95f825ea9cc34e28b0010b8e43d51c92 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T13:45:42Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-95f825ea9cc34e28b0010b8e43d51c922023-11-21T02:37:34ZengMDPI AGMicroorganisms2076-26072020-12-01914410.3390/microorganisms9010044Metabolic Engineering of Extremophilic Bacterium <i>Deinococcus radiodurans</i> for the Production of the Novel Carotenoid DeinoxanthinSun-Wook Jeong0Jun-Ho Kim1Ji-Woong Kim2Chae Yeon Kim3Su Young Kim4Yong Jun Choi5School of Environmental Engineering, University of Seoul, Seoul 02504, KoreaMaterial Sciences Research Institute, LABIO Co., Ltd., Seoul 08501, KoreaMaterial Sciences Research Institute, LABIO Co., Ltd., Seoul 08501, KoreaMaterial Sciences Research Institute, LABIO Co., Ltd., Seoul 08501, KoreaMaterial Sciences Research Institute, LABIO Co., Ltd., Seoul 08501, KoreaSchool of Environmental Engineering, University of Seoul, Seoul 02504, KoreaDeinoxanthin, a xanthophyll derived from <i>Deinococcus</i> species, is a unique organic compound that provides greater antioxidant effects compared to other carotenoids due to its superior scavenging activity against singlet oxygen and hydrogen peroxide. Therefore, it has attracted significant attention as a next-generation organic compound that has great potential as a natural ingredient in a food supplements. Although the microbial identification of deinoxanthin has been identified, mass production has not yet been achieved. Here, we report, for the first time, the development of an engineered extremophilic microorganism, <i>Deinococcus radiodurans</i> strain R1, that is capable of producing deinoxanthin through rational metabolic engineering and process optimization. The genes <i>crtB</i> and <i>dxs</i> were first introduced into the genome to reinforce the metabolic flux towards deinoxanthin. The optimal temperature was then identified through a comparative analysis of the mRNA expression of the two genes, while the carbon source was further optimized to increase deinoxanthin production. The final engineered <i>D. radiodurans</i> strain R1 was able to produce 394 ± 17.6 mg/L (102 ± 11.1 mg/g DCW) of deinoxanthin with a yield of 40.4 ± 1.2 mg/g sucrose and a productivity of 8.4 ± 0.2 mg/L/h from 10 g/L of sucrose. The final engineered strain and the strategies developed in the present study can act as the foundation for the industrial application of extremophilic microorganisms.https://www.mdpi.com/2076-2607/9/1/44<i>Deinococcus radiodurans</i>metabolic engineeringdeinoxanthinxanthophyllsantioxidation |
| spellingShingle | Sun-Wook Jeong Jun-Ho Kim Ji-Woong Kim Chae Yeon Kim Su Young Kim Yong Jun Choi Metabolic Engineering of Extremophilic Bacterium <i>Deinococcus radiodurans</i> for the Production of the Novel Carotenoid Deinoxanthin Microorganisms <i>Deinococcus radiodurans</i> metabolic engineering deinoxanthin xanthophylls antioxidation |
| title | Metabolic Engineering of Extremophilic Bacterium <i>Deinococcus radiodurans</i> for the Production of the Novel Carotenoid Deinoxanthin |
| title_full | Metabolic Engineering of Extremophilic Bacterium <i>Deinococcus radiodurans</i> for the Production of the Novel Carotenoid Deinoxanthin |
| title_fullStr | Metabolic Engineering of Extremophilic Bacterium <i>Deinococcus radiodurans</i> for the Production of the Novel Carotenoid Deinoxanthin |
| title_full_unstemmed | Metabolic Engineering of Extremophilic Bacterium <i>Deinococcus radiodurans</i> for the Production of the Novel Carotenoid Deinoxanthin |
| title_short | Metabolic Engineering of Extremophilic Bacterium <i>Deinococcus radiodurans</i> for the Production of the Novel Carotenoid Deinoxanthin |
| title_sort | metabolic engineering of extremophilic bacterium i deinococcus radiodurans i for the production of the novel carotenoid deinoxanthin |
| topic | <i>Deinococcus radiodurans</i> metabolic engineering deinoxanthin xanthophylls antioxidation |
| url | https://www.mdpi.com/2076-2607/9/1/44 |
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