Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased <i>Akkermansia muciniphila</i> in Mice
Obesity and obesity-related disorders, such as type 2 diabetes have been progressively increasing worldwide and treatments have failed to counteract their progression. Growing evidence have demonstrated that gut microbiota is associated with the incidence of these pathologies. Hence, the identificat...
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MDPI AG
2020-09-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/10/2932 |
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| author | Marion Régnier Marialetizia Rastelli Arianne Morissette Francesco Suriano Tiphaine Le Roy Geneviève Pilon Nathalie M Delzenne André Marette Matthias Van Hul Patrice D Cani |
| author_facet | Marion Régnier Marialetizia Rastelli Arianne Morissette Francesco Suriano Tiphaine Le Roy Geneviève Pilon Nathalie M Delzenne André Marette Matthias Van Hul Patrice D Cani |
| author_sort | Marion Régnier |
| collection | DOAJ |
| description | Obesity and obesity-related disorders, such as type 2 diabetes have been progressively increasing worldwide and treatments have failed to counteract their progression. Growing evidence have demonstrated that gut microbiota is associated with the incidence of these pathologies. Hence, the identification of new nutritional compounds, able to improve health through a modulation of gut microbiota, is gaining interest. In this context, the aim of this study was to investigate the gut-driving effects of rhubarb extract in a context of diet-induced obesity and diabetes. Eight weeks old C57BL6/J male mice were fed a control diet (CTRL), a high fat and high sucrose diet (HFHS) or a HFHS diet supplemented with 0.3% (g/g) of rhubarb extract for eight weeks. Rhubarb supplementation fully prevented HFHS-induced obesity, diabetes, visceral adiposity, adipose tissue inflammation and liver triglyceride accumulation, without any modification in food intake. By combining sequencing and qPCR methods, we found that all these effects were associated with a blooming of <i>Akkermansia muciniphila</i>, which is strongly correlated with increased expression of <i>Reg3γ</i> in the colon. Our data showed that rhubarb supplementation is sufficient to protect against metabolic disorders induced by a diet rich in lipid and carbohydrates in association with a reciprocal interaction between <i>Akkermansia muciniphila</i> and Reg3γ. |
| first_indexed | 2024-03-10T16:04:50Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T16:04:50Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-95face28f605433a8476b1875ce6faf32023-11-20T15:00:47ZengMDPI AGNutrients2072-66432020-09-011210293210.3390/nu12102932Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased <i>Akkermansia muciniphila</i> in MiceMarion Régnier0Marialetizia Rastelli1Arianne Morissette2Francesco Suriano3Tiphaine Le Roy4Geneviève Pilon5Nathalie M Delzenne6André Marette7Matthias Van Hul8Patrice D Cani9Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life sciences and BIOtechnology (WELBIO), UCLouvain, Université catholique de Louvain, Av. E. Mounier, 73 B1.73.11, 1200 Bruxelles, BelgiumMetabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life sciences and BIOtechnology (WELBIO), UCLouvain, Université catholique de Louvain, Av. E. Mounier, 73 B1.73.11, 1200 Bruxelles, BelgiumDepartment of Medicine, Faculty of Medicine, Cardiology Axis of the Quebec Heart and Lung Institute, Quebec, QC G1V 4G5, CanadaMetabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life sciences and BIOtechnology (WELBIO), UCLouvain, Université catholique de Louvain, Av. E. Mounier, 73 B1.73.11, 1200 Bruxelles, BelgiumMetabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life sciences and BIOtechnology (WELBIO), UCLouvain, Université catholique de Louvain, Av. E. Mounier, 73 B1.73.11, 1200 Bruxelles, BelgiumDepartment of Medicine, Faculty of Medicine, Cardiology Axis of the Quebec Heart and Lung Institute, Quebec, QC G1V 4G5, CanadaMetabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life sciences and BIOtechnology (WELBIO), UCLouvain, Université catholique de Louvain, Av. E. Mounier, 73 B1.73.11, 1200 Bruxelles, BelgiumDepartment of Medicine, Faculty of Medicine, Cardiology Axis of the Quebec Heart and Lung Institute, Quebec, QC G1V 4G5, CanadaMetabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life sciences and BIOtechnology (WELBIO), UCLouvain, Université catholique de Louvain, Av. E. Mounier, 73 B1.73.11, 1200 Bruxelles, BelgiumMetabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life sciences and BIOtechnology (WELBIO), UCLouvain, Université catholique de Louvain, Av. E. Mounier, 73 B1.73.11, 1200 Bruxelles, BelgiumObesity and obesity-related disorders, such as type 2 diabetes have been progressively increasing worldwide and treatments have failed to counteract their progression. Growing evidence have demonstrated that gut microbiota is associated with the incidence of these pathologies. Hence, the identification of new nutritional compounds, able to improve health through a modulation of gut microbiota, is gaining interest. In this context, the aim of this study was to investigate the gut-driving effects of rhubarb extract in a context of diet-induced obesity and diabetes. Eight weeks old C57BL6/J male mice were fed a control diet (CTRL), a high fat and high sucrose diet (HFHS) or a HFHS diet supplemented with 0.3% (g/g) of rhubarb extract for eight weeks. Rhubarb supplementation fully prevented HFHS-induced obesity, diabetes, visceral adiposity, adipose tissue inflammation and liver triglyceride accumulation, without any modification in food intake. By combining sequencing and qPCR methods, we found that all these effects were associated with a blooming of <i>Akkermansia muciniphila</i>, which is strongly correlated with increased expression of <i>Reg3γ</i> in the colon. Our data showed that rhubarb supplementation is sufficient to protect against metabolic disorders induced by a diet rich in lipid and carbohydrates in association with a reciprocal interaction between <i>Akkermansia muciniphila</i> and Reg3γ.https://www.mdpi.com/2072-6643/12/10/2932Rhubarbobesitydiabetesgut microbiota<i>Akkermansia muciniphila</i>Reg3γ |
| spellingShingle | Marion Régnier Marialetizia Rastelli Arianne Morissette Francesco Suriano Tiphaine Le Roy Geneviève Pilon Nathalie M Delzenne André Marette Matthias Van Hul Patrice D Cani Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased <i>Akkermansia muciniphila</i> in Mice Nutrients Rhubarb obesity diabetes gut microbiota <i>Akkermansia muciniphila</i> Reg3γ |
| title | Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased <i>Akkermansia muciniphila</i> in Mice |
| title_full | Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased <i>Akkermansia muciniphila</i> in Mice |
| title_fullStr | Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased <i>Akkermansia muciniphila</i> in Mice |
| title_full_unstemmed | Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased <i>Akkermansia muciniphila</i> in Mice |
| title_short | Rhubarb Supplementation Prevents Diet-Induced Obesity and Diabetes in Association with Increased <i>Akkermansia muciniphila</i> in Mice |
| title_sort | rhubarb supplementation prevents diet induced obesity and diabetes in association with increased i akkermansia muciniphila i in mice |
| topic | Rhubarb obesity diabetes gut microbiota <i>Akkermansia muciniphila</i> Reg3γ |
| url | https://www.mdpi.com/2072-6643/12/10/2932 |
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