promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis
Objective This study explored the mechanism of squamous cervical cancer (SCC) progression. Methods Reverse transcription-quantitative polymerase chain reaction and western blotting were used to evaluate the expression of myosin light chain 9 ( MYL9 ) in SCC tissues and cell lines. Furthermore, Trans...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2023-11-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/03000605231208582 |
| _version_ | 1797630692044046336 |
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| author | Bin Wen Limei Luo Zhaoyang Zeng Xiping Luo |
| author_facet | Bin Wen Limei Luo Zhaoyang Zeng Xiping Luo |
| author_sort | Bin Wen |
| collection | DOAJ |
| description | Objective This study explored the mechanism of squamous cervical cancer (SCC) progression. Methods Reverse transcription-quantitative polymerase chain reaction and western blotting were used to evaluate the expression of myosin light chain 9 ( MYL9 ) in SCC tissues and cell lines. Furthermore, Transwell and Boyden assays were used to assess the function of MYL9 in SCC progression. In addition, the levels of lactate and aerobic glycolysis were used to explore the detailed mechanism of MYL9 in SCC. Results The mRNA and protein levels of MYL9 were elevated in SCC tissues, and MYL9 knockdown inhibited the migration and invasion of SCC cell lines. A mechanistic study demonstrated that MYL9 promotes SCC migration and invasion by enhancing aerobic glycolysis and increasing the activity of the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway. Conclusions MYL9 was upregulated in SCC, and it enhanced JAK2/STAT3 pathway activity and promoted metastasis and glycolysis in SCC. |
| first_indexed | 2024-03-11T11:11:47Z |
| format | Article |
| id | doaj.art-95fb3467aca54179b89cc97af05ee9ed |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-03-11T11:11:47Z |
| publishDate | 2023-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-95fb3467aca54179b89cc97af05ee9ed2023-11-11T23:33:19ZengSAGE PublishingJournal of International Medical Research1473-23002023-11-015110.1177/03000605231208582 promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysisBin WenLimei LuoZhaoyang ZengXiping LuoObjective This study explored the mechanism of squamous cervical cancer (SCC) progression. Methods Reverse transcription-quantitative polymerase chain reaction and western blotting were used to evaluate the expression of myosin light chain 9 ( MYL9 ) in SCC tissues and cell lines. Furthermore, Transwell and Boyden assays were used to assess the function of MYL9 in SCC progression. In addition, the levels of lactate and aerobic glycolysis were used to explore the detailed mechanism of MYL9 in SCC. Results The mRNA and protein levels of MYL9 were elevated in SCC tissues, and MYL9 knockdown inhibited the migration and invasion of SCC cell lines. A mechanistic study demonstrated that MYL9 promotes SCC migration and invasion by enhancing aerobic glycolysis and increasing the activity of the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway. Conclusions MYL9 was upregulated in SCC, and it enhanced JAK2/STAT3 pathway activity and promoted metastasis and glycolysis in SCC.https://doi.org/10.1177/03000605231208582 |
| spellingShingle | Bin Wen Limei Luo Zhaoyang Zeng Xiping Luo promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis Journal of International Medical Research |
| title | promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis |
| title_full | promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis |
| title_fullStr | promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis |
| title_full_unstemmed | promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis |
| title_short | promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis |
| title_sort | promotes squamous cervical cancer migration and invasion by enhancing aerobic glycolysis |
| url | https://doi.org/10.1177/03000605231208582 |
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