Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease
Severe malaria (SM) is a major public health problem in malaria-endemic countries. Sequestration of Plasmodium falciparum–infected erythrocytes in vital organs and the associated inflammation leads to organ dysfunction. MicroRNAs (miRNAs), which are rapidly released from damaged tissues into the hos...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2021-02-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/27/2/19-1795_article |
| _version_ | 1819242420963901440 |
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| author | Himanshu Gupta Mercedes Rubio Antonio Sitoe Rosauro Varo Pau Cisteró Lola Madrid Inocencia Cuamba Alfons Jimenez Xavier Martiáñez-Vendrell Diana Barrios Lorena Pantano Allison Brimacombe Mariona Bustamante Quique Bassat Alfredo Mayor |
| author_facet | Himanshu Gupta Mercedes Rubio Antonio Sitoe Rosauro Varo Pau Cisteró Lola Madrid Inocencia Cuamba Alfons Jimenez Xavier Martiáñez-Vendrell Diana Barrios Lorena Pantano Allison Brimacombe Mariona Bustamante Quique Bassat Alfredo Mayor |
| author_sort | Himanshu Gupta |
| collection | DOAJ |
| description | Severe malaria (SM) is a major public health problem in malaria-endemic countries. Sequestration of Plasmodium falciparum–infected erythrocytes in vital organs and the associated inflammation leads to organ dysfunction. MicroRNAs (miRNAs), which are rapidly released from damaged tissues into the host fluids, constitute a promising biomarker for the prognosis of SM. We applied next-generation sequencing to evaluate the differential expression of miRNAs in SM and in uncomplicated malaria (UM) in children in Mozambique. Six miRNAs were associated with in vitro P. falciparum cytoadhesion, severity in children, and P. falciparum biomass. Relative expression of hsa-miR-4497 quantified by TaqMan-quantitative reverse transcription PCR was higher in plasma of children with SM than those with UM (p<0.048) and again correlated with P. falciparum biomass (p = 0.033). These findings suggest that different physiopathological processes in SM and UM lead to differential expression of miRNAs and suggest a pathway for assessing their prognostic value malaria. |
| first_indexed | 2024-12-23T14:39:32Z |
| format | Article |
| id | doaj.art-95fe2e9beb2342e2a79de088dedac99d |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-23T14:39:32Z |
| publishDate | 2021-02-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-95fe2e9beb2342e2a79de088dedac99d2022-12-21T17:43:16ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592021-02-0127243044210.3201/eid2702.191795Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria DiseaseHimanshu GuptaMercedes RubioAntonio SitoeRosauro VaroPau CisteróLola MadridInocencia CuambaAlfons JimenezXavier Martiáñez-VendrellDiana BarriosLorena PantanoAllison BrimacombeMariona BustamanteQuique BassatAlfredo MayorSevere malaria (SM) is a major public health problem in malaria-endemic countries. Sequestration of Plasmodium falciparum–infected erythrocytes in vital organs and the associated inflammation leads to organ dysfunction. MicroRNAs (miRNAs), which are rapidly released from damaged tissues into the host fluids, constitute a promising biomarker for the prognosis of SM. We applied next-generation sequencing to evaluate the differential expression of miRNAs in SM and in uncomplicated malaria (UM) in children in Mozambique. Six miRNAs were associated with in vitro P. falciparum cytoadhesion, severity in children, and P. falciparum biomass. Relative expression of hsa-miR-4497 quantified by TaqMan-quantitative reverse transcription PCR was higher in plasma of children with SM than those with UM (p<0.048) and again correlated with P. falciparum biomass (p = 0.033). These findings suggest that different physiopathological processes in SM and UM lead to differential expression of miRNAs and suggest a pathway for assessing their prognostic value malaria.https://wwwnc.cdc.gov/eid/article/27/2/19-1795_articlePlasmodium falciparummiRNAmalariasevere malariabiomarkersnext-generation sequencing |
| spellingShingle | Himanshu Gupta Mercedes Rubio Antonio Sitoe Rosauro Varo Pau Cisteró Lola Madrid Inocencia Cuamba Alfons Jimenez Xavier Martiáñez-Vendrell Diana Barrios Lorena Pantano Allison Brimacombe Mariona Bustamante Quique Bassat Alfredo Mayor Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease Emerging Infectious Diseases Plasmodium falciparum miRNA malaria severe malaria biomarkers next-generation sequencing |
| title | Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease |
| title_full | Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease |
| title_fullStr | Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease |
| title_full_unstemmed | Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease |
| title_short | Plasma MicroRNA Profiling of Plasmodium falciparum Biomass and Association with Severity of Malaria Disease |
| title_sort | plasma microrna profiling of plasmodium falciparum biomass and association with severity of malaria disease |
| topic | Plasmodium falciparum miRNA malaria severe malaria biomarkers next-generation sequencing |
| url | https://wwwnc.cdc.gov/eid/article/27/2/19-1795_article |
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