Identification of Escherichia coli multidrug resistance transporters involved in anthocyanin biosynthesis
The anthocyanin compound cyanidin 3-O-glucoside (C3G) is a natural pigment widely used in food and nutraceutical industries. Its microbial synthesis by E. coli is a promising alternative to the traditional extraction methods. However, part of the synthesized C3G accumulates in the cytoplasm, thus po...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1357794/full |
| _version_ | 1797221712064937984 |
|---|---|
| author | Xia Wu Xia Wu Rongxia Chen Ping Liang Jian Zha Jian Zha |
| author_facet | Xia Wu Xia Wu Rongxia Chen Ping Liang Jian Zha Jian Zha |
| author_sort | Xia Wu |
| collection | DOAJ |
| description | The anthocyanin compound cyanidin 3-O-glucoside (C3G) is a natural pigment widely used in food and nutraceutical industries. Its microbial synthesis by E. coli is a promising alternative to the traditional extraction methods. However, part of the synthesized C3G accumulates in the cytoplasm, thus potentially causing growth inhibition and product degradation. Therefore, it is necessary to enhance C3G secretion via exploration of native transporters facilitating C3G export. In this study, we report the screening and verification of native multidrug resistance transporters from 40 candidates in E. coli that can improve the extracellular C3G production when using catechin as the substrate. Overexpression of single transporter genes including fsr, yebQ, ynfM, mdlAB, and emrKY were found to increase C3G production by 0.5- to 4.8-fold. Genetic studies indicated that mdlAB and emrKY are vital transporters in the secretion of C3G. Our study reveals a set of new multidrug resistance transporters for the improvement of microbial biosynthesis of C3G and other anthocyanins. |
| first_indexed | 2024-04-24T13:09:47Z |
| format | Article |
| id | doaj.art-9601941bc5e24bb781ee4dd387056620 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T13:09:47Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9601941bc5e24bb781ee4dd3870566202024-04-05T04:48:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-04-011510.3389/fmicb.2024.13577941357794Identification of Escherichia coli multidrug resistance transporters involved in anthocyanin biosynthesisXia Wu0Xia Wu1Rongxia Chen2Ping Liang3Jian Zha4Jian Zha5School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an, ChinaXi’an Key Laboratory of Antiviral and Antimicrobial-Resistant Bacteria Therapeutics Research, Xi’an, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an, ChinaXi’an Key Laboratory of Antiviral and Antimicrobial-Resistant Bacteria Therapeutics Research, Xi’an, ChinaThe anthocyanin compound cyanidin 3-O-glucoside (C3G) is a natural pigment widely used in food and nutraceutical industries. Its microbial synthesis by E. coli is a promising alternative to the traditional extraction methods. However, part of the synthesized C3G accumulates in the cytoplasm, thus potentially causing growth inhibition and product degradation. Therefore, it is necessary to enhance C3G secretion via exploration of native transporters facilitating C3G export. In this study, we report the screening and verification of native multidrug resistance transporters from 40 candidates in E. coli that can improve the extracellular C3G production when using catechin as the substrate. Overexpression of single transporter genes including fsr, yebQ, ynfM, mdlAB, and emrKY were found to increase C3G production by 0.5- to 4.8-fold. Genetic studies indicated that mdlAB and emrKY are vital transporters in the secretion of C3G. Our study reveals a set of new multidrug resistance transporters for the improvement of microbial biosynthesis of C3G and other anthocyanins.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1357794/fullcyanidin 3-O-glucosideefflux transporteranthocyaninE. colimultidrug resistance transporters |
| spellingShingle | Xia Wu Xia Wu Rongxia Chen Ping Liang Jian Zha Jian Zha Identification of Escherichia coli multidrug resistance transporters involved in anthocyanin biosynthesis Frontiers in Microbiology cyanidin 3-O-glucoside efflux transporter anthocyanin E. coli multidrug resistance transporters |
| title | Identification of Escherichia coli multidrug resistance transporters involved in anthocyanin biosynthesis |
| title_full | Identification of Escherichia coli multidrug resistance transporters involved in anthocyanin biosynthesis |
| title_fullStr | Identification of Escherichia coli multidrug resistance transporters involved in anthocyanin biosynthesis |
| title_full_unstemmed | Identification of Escherichia coli multidrug resistance transporters involved in anthocyanin biosynthesis |
| title_short | Identification of Escherichia coli multidrug resistance transporters involved in anthocyanin biosynthesis |
| title_sort | identification of escherichia coli multidrug resistance transporters involved in anthocyanin biosynthesis |
| topic | cyanidin 3-O-glucoside efflux transporter anthocyanin E. coli multidrug resistance transporters |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1357794/full |
| work_keys_str_mv | AT xiawu identificationofescherichiacolimultidrugresistancetransportersinvolvedinanthocyaninbiosynthesis AT xiawu identificationofescherichiacolimultidrugresistancetransportersinvolvedinanthocyaninbiosynthesis AT rongxiachen identificationofescherichiacolimultidrugresistancetransportersinvolvedinanthocyaninbiosynthesis AT pingliang identificationofescherichiacolimultidrugresistancetransportersinvolvedinanthocyaninbiosynthesis AT jianzha identificationofescherichiacolimultidrugresistancetransportersinvolvedinanthocyaninbiosynthesis AT jianzha identificationofescherichiacolimultidrugresistancetransportersinvolvedinanthocyaninbiosynthesis |