Screening plant growth-promoting bacteria from the rhizosphere of invasive weed Ageratina adenophora for crop growth
Plant-growth promoting rhizobacteria (PGPR) play a vital role in soil fertility and crop production. The rhizosphere of many crop plants has been well documented by screening PGPR for their plant-growth promoting (PGP) mechanisms. However, the rhizosphere of grass species that may act as potential h...
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PeerJ Inc.
2023-03-01
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| author | Yun Xia Hongbo Zhang Yu Zhang Yuyu Zhang Jiani Liu Robert Seviour Yunhong Kong |
| author_facet | Yun Xia Hongbo Zhang Yu Zhang Yuyu Zhang Jiani Liu Robert Seviour Yunhong Kong |
| author_sort | Yun Xia |
| collection | DOAJ |
| description | Plant-growth promoting rhizobacteria (PGPR) play a vital role in soil fertility and crop production. The rhizosphere of many crop plants has been well documented by screening PGPR for their plant-growth promoting (PGP) mechanisms. However, the rhizosphere of grass species that may act as potential habitats for novel PGPR remains relatively unexplored. Ageratina adenophora is a noxious weed that has invaded more than 40 tropical and subtropical countries in Asia, Oceania, Africa, and Europe. Its presence has led to changes in plant species composition, reducing their biodiversity and destroying ecosystem function. In this study, we screened 1,200 bacterial strains isolated from the rhizosphere soil of A. adenophora in three floristic regions in Yunnan Province, China. Samples were screened for their in vitro ability for N-fixation, production of the plant growth regulator indole-3-acetic acid (IAA), and the synthesis of 1-amino-cyclopropane-1-carboxylate (ACC) deaminase, which controls the levels of ethylene in developing plant roots. We found that 144 strains showed at least one of these PGP attributes. 16S rRNA gene sequencing showed that most (62.5%) of the samples were bacteria closely related to members of the genera Pseudomonas (27 strains), Providencia (20 strains), Chryseobacterium (14 strains), Ensifer (12 strains), Enterobacter (nine strains), and Hafnia (eight strains). Their abundance and biodiversity in the soil of individual floristic regions correlate positively with the invasion history of A. adenophora. From these PGP bacterial strains, KM_A34 (Pantoea agglomerans), KM_C04 (Enterobacter asburiae), and KM_A57 (Pseudomonas putida), which had the greatest in vitro ability of N-fixation, and IAA and ACC deaminase production, respectively, were selected. The strains were evaluated for their effect on the seed germination and growth of soybean, faba bean, pea, wheat, and Chinese cabbage other than A. adenophora. Chamber experiments showed these strains significantly (P < 0.05) increased (14.2–43.4% over the controls) germination rates of the soybean, faba bean, pea, and/or Chinese cabbage seeds. They also reduced relative seed germination times (20.8–48.8% over the controls) of soy bean, faba bean and/or wheat seeds. Greenhouse pot experiments showed that they significantly (P < 0.05) promoted the aboveground and belowground height of plant foliage (12.1–23.1% and 11.5–31.4% over the controls, respectively) and/or the dry weights (16.1–33.5% and 10.6–23.4% over the controls, respectively) of the soy bean, faba bean, pea, wheat and/or Chinese cabbage. These data indicate that the rhizosphere microbiota of A. adenophora contain a PGPR pool that may be used as bioinoculants to improve the growth and productivity of these crops. |
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| spelling | doaj.art-9601c67f2a79457b8c47e3a3009a3b722023-12-03T01:07:33ZengPeerJ Inc.PeerJ2167-83592023-03-0111e1506410.7717/peerj.15064Screening plant growth-promoting bacteria from the rhizosphere of invasive weed Ageratina adenophora for crop growthYun Xia0Hongbo Zhang1Yu Zhang2Yuyu Zhang3Jiani Liu4Robert Seviour5Yunhong Kong6Yunnan Urban Agricultural Engineering & Technological Research Centre, Kunming University, Kunming, Yunnan, ChinaSchool of Agriculture and Biotechnology, Kunming University, Kunming, Yunnan, ChinaSchool of Agriculture and Biotechnology, Kunming University, Kunming, Yunnan, ChinaSchool of Agriculture and Biotechnology, Kunming University, Kunming, Yunnan, ChinaSchool of Agriculture and Biotechnology, Kunming University, Kunming, Yunnan, ChinaMicrobiology Department, La Trobe University, Bundoora, Victoria, AustraliaKunming Key laboratory of Hydro-ecology Restoration of Dianchi Lake, Kunming University, Kunming, Yunnan, ChinaPlant-growth promoting rhizobacteria (PGPR) play a vital role in soil fertility and crop production. The rhizosphere of many crop plants has been well documented by screening PGPR for their plant-growth promoting (PGP) mechanisms. However, the rhizosphere of grass species that may act as potential habitats for novel PGPR remains relatively unexplored. Ageratina adenophora is a noxious weed that has invaded more than 40 tropical and subtropical countries in Asia, Oceania, Africa, and Europe. Its presence has led to changes in plant species composition, reducing their biodiversity and destroying ecosystem function. In this study, we screened 1,200 bacterial strains isolated from the rhizosphere soil of A. adenophora in three floristic regions in Yunnan Province, China. Samples were screened for their in vitro ability for N-fixation, production of the plant growth regulator indole-3-acetic acid (IAA), and the synthesis of 1-amino-cyclopropane-1-carboxylate (ACC) deaminase, which controls the levels of ethylene in developing plant roots. We found that 144 strains showed at least one of these PGP attributes. 16S rRNA gene sequencing showed that most (62.5%) of the samples were bacteria closely related to members of the genera Pseudomonas (27 strains), Providencia (20 strains), Chryseobacterium (14 strains), Ensifer (12 strains), Enterobacter (nine strains), and Hafnia (eight strains). Their abundance and biodiversity in the soil of individual floristic regions correlate positively with the invasion history of A. adenophora. From these PGP bacterial strains, KM_A34 (Pantoea agglomerans), KM_C04 (Enterobacter asburiae), and KM_A57 (Pseudomonas putida), which had the greatest in vitro ability of N-fixation, and IAA and ACC deaminase production, respectively, were selected. The strains were evaluated for their effect on the seed germination and growth of soybean, faba bean, pea, wheat, and Chinese cabbage other than A. adenophora. Chamber experiments showed these strains significantly (P < 0.05) increased (14.2–43.4% over the controls) germination rates of the soybean, faba bean, pea, and/or Chinese cabbage seeds. They also reduced relative seed germination times (20.8–48.8% over the controls) of soy bean, faba bean and/or wheat seeds. Greenhouse pot experiments showed that they significantly (P < 0.05) promoted the aboveground and belowground height of plant foliage (12.1–23.1% and 11.5–31.4% over the controls, respectively) and/or the dry weights (16.1–33.5% and 10.6–23.4% over the controls, respectively) of the soy bean, faba bean, pea, wheat and/or Chinese cabbage. These data indicate that the rhizosphere microbiota of A. adenophora contain a PGPR pool that may be used as bioinoculants to improve the growth and productivity of these crops.https://peerj.com/articles/15064.pdfPlant growth-promoting rhizobacteriaAgeratina adenophoraN-fixationIndole-3-acetic acid productionAmino-cyclopropane-1-carboxylate deaminase productionGreenhouse pot experiment |
| spellingShingle | Yun Xia Hongbo Zhang Yu Zhang Yuyu Zhang Jiani Liu Robert Seviour Yunhong Kong Screening plant growth-promoting bacteria from the rhizosphere of invasive weed Ageratina adenophora for crop growth PeerJ Plant growth-promoting rhizobacteria Ageratina adenophora N-fixation Indole-3-acetic acid production Amino-cyclopropane-1-carboxylate deaminase production Greenhouse pot experiment |
| title | Screening plant growth-promoting bacteria from the rhizosphere of invasive weed Ageratina adenophora for crop growth |
| title_full | Screening plant growth-promoting bacteria from the rhizosphere of invasive weed Ageratina adenophora for crop growth |
| title_fullStr | Screening plant growth-promoting bacteria from the rhizosphere of invasive weed Ageratina adenophora for crop growth |
| title_full_unstemmed | Screening plant growth-promoting bacteria from the rhizosphere of invasive weed Ageratina adenophora for crop growth |
| title_short | Screening plant growth-promoting bacteria from the rhizosphere of invasive weed Ageratina adenophora for crop growth |
| title_sort | screening plant growth promoting bacteria from the rhizosphere of invasive weed ageratina adenophora for crop growth |
| topic | Plant growth-promoting rhizobacteria Ageratina adenophora N-fixation Indole-3-acetic acid production Amino-cyclopropane-1-carboxylate deaminase production Greenhouse pot experiment |
| url | https://peerj.com/articles/15064.pdf |
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