Bioactivity-Guided Isolation of Phytochemicals from <i>Vaccinium dunalianum</i> Wight and Their Antioxidant and Enzyme Inhibitory Activities
<i>Vaccinium dunalianum</i> Wight, usually processed as a traditional folk tea beverage, is widely distributed in the southwest of China. The present study aimed to investigate the antioxidant, α-glucosidase and pancreatic lipase inhibitory activities of <i>V.</i><i>dun...
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MDPI AG
2021-04-01
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| Online Access: | https://www.mdpi.com/1420-3049/26/7/2075 |
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| author | Tianrui Zhao Mengxue Sun Lingpeng Kong Qingwang Xue Yudan Wang Yifen Wang Afsar Khan Jianxin Cao Guiguang Cheng |
| author_facet | Tianrui Zhao Mengxue Sun Lingpeng Kong Qingwang Xue Yudan Wang Yifen Wang Afsar Khan Jianxin Cao Guiguang Cheng |
| author_sort | Tianrui Zhao |
| collection | DOAJ |
| description | <i>Vaccinium dunalianum</i> Wight, usually processed as a traditional folk tea beverage, is widely distributed in the southwest of China. The present study aimed to investigate the antioxidant, α-glucosidase and pancreatic lipase inhibitory activities of <i>V.</i><i>dunalianum</i> extract and isolate the bioactive components. In this study, the crude extract (CE) from the buds of <i>V. dunalianum</i> was prepared by the ultrasound-assisted extraction method in 70% methanol and then purified with macroporous resin D101 to obtain the purified extract (PM). Five fractions (Fr. A–E) were further obtained by MPLC column (RP-C18). Bioactivity assays revealed that Fr. B with 40% methanol and Fr. D with 80% methanol had better antioxidant with 0.48 ± 0.03 and 0.62 ± 0.01 nM Trolox equivalent (TE)/mg extract for DPPH, 0.87 ± 0.02 and 1.58 ± 0.02 nM TE/mg extract for FRAP, 14.42 ± 0.41 and 19.25 ± 0.23 nM TE/mg extract for ABTS, and enzyme inhibitory effects with IC<sub>50</sub> values of 95.21 ± 2.21 and 74.55 ± 3.85 for <i>α</i>-glucosidase, and 142.53 ± 11.45 and 128.76 ± 13.85 µg/mL for pancreatic lipase. Multivariate analysis indicated that the TPC and TFC were positively related to the antioxidant activities. Further phytochemical purification led to the isolation of ten compounds (1–10). 6-<i>O</i>-Caffeoylarbutin (7) showed significant inhibitory effects on <i>α</i>-glucosidase and pancreatic lipase enzymes with values of 38.38 ± 1.84 and 97.56 ± 7.53 µg/mL, and had the highest antioxidant capacity compared to the other compounds. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:36:52Z |
| publishDate | 2021-04-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-960390412f34476ba787484bb0bd0fb42023-11-21T14:11:55ZengMDPI AGMolecules1420-30492021-04-01267207510.3390/molecules26072075Bioactivity-Guided Isolation of Phytochemicals from <i>Vaccinium dunalianum</i> Wight and Their Antioxidant and Enzyme Inhibitory ActivitiesTianrui Zhao0Mengxue Sun1Lingpeng Kong2Qingwang Xue3Yudan Wang4Yifen Wang5Afsar Khan6Jianxin Cao7Guiguang Cheng8Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, ChinaDepartment of Chemistry, Liaocheng University, Liaocheng 252059, ChinaFaculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, ChinaKunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650000, ChinaDepartment of Chemistry, COMSATS University Islamabad, Abbottabad Campus, Abbottabad 22060, PakistanFaculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Agriculture and Food, Kunming University of Science and Technology, Kunming 650500, China<i>Vaccinium dunalianum</i> Wight, usually processed as a traditional folk tea beverage, is widely distributed in the southwest of China. The present study aimed to investigate the antioxidant, α-glucosidase and pancreatic lipase inhibitory activities of <i>V.</i><i>dunalianum</i> extract and isolate the bioactive components. In this study, the crude extract (CE) from the buds of <i>V. dunalianum</i> was prepared by the ultrasound-assisted extraction method in 70% methanol and then purified with macroporous resin D101 to obtain the purified extract (PM). Five fractions (Fr. A–E) were further obtained by MPLC column (RP-C18). Bioactivity assays revealed that Fr. B with 40% methanol and Fr. D with 80% methanol had better antioxidant with 0.48 ± 0.03 and 0.62 ± 0.01 nM Trolox equivalent (TE)/mg extract for DPPH, 0.87 ± 0.02 and 1.58 ± 0.02 nM TE/mg extract for FRAP, 14.42 ± 0.41 and 19.25 ± 0.23 nM TE/mg extract for ABTS, and enzyme inhibitory effects with IC<sub>50</sub> values of 95.21 ± 2.21 and 74.55 ± 3.85 for <i>α</i>-glucosidase, and 142.53 ± 11.45 and 128.76 ± 13.85 µg/mL for pancreatic lipase. Multivariate analysis indicated that the TPC and TFC were positively related to the antioxidant activities. Further phytochemical purification led to the isolation of ten compounds (1–10). 6-<i>O</i>-Caffeoylarbutin (7) showed significant inhibitory effects on <i>α</i>-glucosidase and pancreatic lipase enzymes with values of 38.38 ± 1.84 and 97.56 ± 7.53 µg/mL, and had the highest antioxidant capacity compared to the other compounds.https://www.mdpi.com/1420-3049/26/7/2075<i>Vaccinium dunalianum</i>antioxidantenzyme inhibitoryguided isolation |
| spellingShingle | Tianrui Zhao Mengxue Sun Lingpeng Kong Qingwang Xue Yudan Wang Yifen Wang Afsar Khan Jianxin Cao Guiguang Cheng Bioactivity-Guided Isolation of Phytochemicals from <i>Vaccinium dunalianum</i> Wight and Their Antioxidant and Enzyme Inhibitory Activities Molecules <i>Vaccinium dunalianum</i> antioxidant enzyme inhibitory guided isolation |
| title | Bioactivity-Guided Isolation of Phytochemicals from <i>Vaccinium dunalianum</i> Wight and Their Antioxidant and Enzyme Inhibitory Activities |
| title_full | Bioactivity-Guided Isolation of Phytochemicals from <i>Vaccinium dunalianum</i> Wight and Their Antioxidant and Enzyme Inhibitory Activities |
| title_fullStr | Bioactivity-Guided Isolation of Phytochemicals from <i>Vaccinium dunalianum</i> Wight and Their Antioxidant and Enzyme Inhibitory Activities |
| title_full_unstemmed | Bioactivity-Guided Isolation of Phytochemicals from <i>Vaccinium dunalianum</i> Wight and Their Antioxidant and Enzyme Inhibitory Activities |
| title_short | Bioactivity-Guided Isolation of Phytochemicals from <i>Vaccinium dunalianum</i> Wight and Their Antioxidant and Enzyme Inhibitory Activities |
| title_sort | bioactivity guided isolation of phytochemicals from i vaccinium dunalianum i wight and their antioxidant and enzyme inhibitory activities |
| topic | <i>Vaccinium dunalianum</i> antioxidant enzyme inhibitory guided isolation |
| url | https://www.mdpi.com/1420-3049/26/7/2075 |
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