Prevalence, phenotype and genotype characteristics of antibiotic resistance in coastal beach practitioners of tropical China
Objective: To investigate the genetic mechanism and prevalence of antibiotic resistance among the practitioners of two bathing beaches in tropical China. Methods: Totally 259 strains were isolated from the rectal swabs of the practitioners and screened by MacConkey agar. A questionnaire survey was c...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | One Health Bulletin |
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| Online Access: | http://www.johb.info/article.asp?issn=2773-0344;year=2022;volume=2;issue=1;spage=2;epage=2;aulast=Long |
| _version_ | 1797322303850151936 |
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| author | Wenfang Long Tianjiao Li Guohui Yi Feng Liang Gaoyao Hu Jizhen Wu Hairong Huang Mingxue Li |
| author_facet | Wenfang Long Tianjiao Li Guohui Yi Feng Liang Gaoyao Hu Jizhen Wu Hairong Huang Mingxue Li |
| author_sort | Wenfang Long |
| collection | DOAJ |
| description | Objective: To investigate the genetic mechanism and prevalence of antibiotic resistance among the practitioners of two bathing beaches in tropical China.
Methods: Totally 259 strains were isolated from the rectal swabs of the practitioners and screened by MacConkey agar. A questionnaire survey was conducted. Kirby-Bauer test was used for phenotype, and double disc inhibition synergy test was used for extended-spectrum β -lactamase-producing Enterobacteriaceae (ESBL-E), which was further confirmed by VITEKII instrument. The genotype was detected by polymerase chain reaction, and the similarities of ESBL-E were analyzed using pulsed-field gel electrophoresis.
Results: The prevalence of ESBL-E was 13.1% (34/259), of which, CTX-M genes accounted for 44.1% (15/34), mainly CTX-M-14 and CTX-M-27. Moreover, 19.1% (4/21) of the isolates were homologous. The resistance phenotypes of ESBL-E to chloramphenicol, ciprofloxacin, levofloxacin, ceftazidime, cefepime and gentamicin were different between two beaches(P<0.05). The prevalence of ESBL-E was caused by CTX-M genes at the island beach, while a high prevalence of ESBL-E was found at the city beach and clone transfer occurred.
Conclusions: ESBL-E-associated gentamicin antibiotic resistance risk may be greater for island beach practitioners. The city beach populations could face more potential risks owing to severe resistance and metastatic ability of ESBL-E. The environmental exposure mechanisms and transfer role of antibiotic resistance at public beaches with high density should be investigated. |
| first_indexed | 2024-03-08T05:11:20Z |
| format | Article |
| id | doaj.art-9603fbf0010c4a32aaf5f77517d367f9 |
| institution | Directory Open Access Journal |
| issn | 2773-0344 2773-0352 |
| language | English |
| last_indexed | 2024-03-08T05:11:20Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | One Health Bulletin |
| spelling | doaj.art-9603fbf0010c4a32aaf5f77517d367f92024-02-07T09:10:01ZengWolters Kluwer Medknow PublicationsOne Health Bulletin2773-03442773-03522022-01-01212210.4103/2773-0344.343630Prevalence, phenotype and genotype characteristics of antibiotic resistance in coastal beach practitioners of tropical ChinaWenfang LongTianjiao LiGuohui YiFeng LiangGaoyao HuJizhen WuHairong HuangMingxue LiObjective: To investigate the genetic mechanism and prevalence of antibiotic resistance among the practitioners of two bathing beaches in tropical China. Methods: Totally 259 strains were isolated from the rectal swabs of the practitioners and screened by MacConkey agar. A questionnaire survey was conducted. Kirby-Bauer test was used for phenotype, and double disc inhibition synergy test was used for extended-spectrum β -lactamase-producing Enterobacteriaceae (ESBL-E), which was further confirmed by VITEKII instrument. The genotype was detected by polymerase chain reaction, and the similarities of ESBL-E were analyzed using pulsed-field gel electrophoresis. Results: The prevalence of ESBL-E was 13.1% (34/259), of which, CTX-M genes accounted for 44.1% (15/34), mainly CTX-M-14 and CTX-M-27. Moreover, 19.1% (4/21) of the isolates were homologous. The resistance phenotypes of ESBL-E to chloramphenicol, ciprofloxacin, levofloxacin, ceftazidime, cefepime and gentamicin were different between two beaches(P<0.05). The prevalence of ESBL-E was caused by CTX-M genes at the island beach, while a high prevalence of ESBL-E was found at the city beach and clone transfer occurred. Conclusions: ESBL-E-associated gentamicin antibiotic resistance risk may be greater for island beach practitioners. The city beach populations could face more potential risks owing to severe resistance and metastatic ability of ESBL-E. The environmental exposure mechanisms and transfer role of antibiotic resistance at public beaches with high density should be investigated.http://www.johb.info/article.asp?issn=2773-0344;year=2022;volume=2;issue=1;spage=2;epage=2;aulast=Longantibiotic resistance; antibiotic resistance genes; extended-spectrum β -lactamase-producing enterobacteriaceae; beach practitioners; risk |
| spellingShingle | Wenfang Long Tianjiao Li Guohui Yi Feng Liang Gaoyao Hu Jizhen Wu Hairong Huang Mingxue Li Prevalence, phenotype and genotype characteristics of antibiotic resistance in coastal beach practitioners of tropical China One Health Bulletin antibiotic resistance; antibiotic resistance genes; extended-spectrum β -lactamase-producing enterobacteriaceae; beach practitioners; risk |
| title | Prevalence, phenotype and genotype characteristics of antibiotic resistance in coastal beach practitioners of tropical China |
| title_full | Prevalence, phenotype and genotype characteristics of antibiotic resistance in coastal beach practitioners of tropical China |
| title_fullStr | Prevalence, phenotype and genotype characteristics of antibiotic resistance in coastal beach practitioners of tropical China |
| title_full_unstemmed | Prevalence, phenotype and genotype characteristics of antibiotic resistance in coastal beach practitioners of tropical China |
| title_short | Prevalence, phenotype and genotype characteristics of antibiotic resistance in coastal beach practitioners of tropical China |
| title_sort | prevalence phenotype and genotype characteristics of antibiotic resistance in coastal beach practitioners of tropical china |
| topic | antibiotic resistance; antibiotic resistance genes; extended-spectrum β -lactamase-producing enterobacteriaceae; beach practitioners; risk |
| url | http://www.johb.info/article.asp?issn=2773-0344;year=2022;volume=2;issue=1;spage=2;epage=2;aulast=Long |
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