Long-term supplementation of esculetin ameliorates hepatosteatosis and insulin resistance partly by activating AdipoR2–AMPK pathway in diet-induced obese mice
The effects of esculetin on hepatosteatosis and insulin resistance in high-fat diet (HFD)-induced obese mice were investigated. Esculetin (0.02%, w/w) provided to mice fed a HFD (40% kcal from fat) for 12 weeks reduced body weight gain, visceral adiposity, hepatosteatosis, hyperlipidemia and hypergl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-05-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S175646461500122X |
| _version_ | 1819294299195441152 |
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| author | Mi-Ok Sim Hae-In Lee Ju Ri Ham Kwon-Il Seo Mi-Kyung Lee |
| author_facet | Mi-Ok Sim Hae-In Lee Ju Ri Ham Kwon-Il Seo Mi-Kyung Lee |
| author_sort | Mi-Ok Sim |
| collection | DOAJ |
| description | The effects of esculetin on hepatosteatosis and insulin resistance in high-fat diet (HFD)-induced obese mice were investigated. Esculetin (0.02%, w/w) provided to mice fed a HFD (40% kcal from fat) for 12 weeks reduced body weight gain, visceral adiposity, hepatosteatosis, hyperlipidemia and hyperglycemia, whereas it increased plasma adiponectin level and the protein and mRNA expression of hepatic AdipoR2, which led to the activation of AMPK. The protein and mRNA expression of hepatic PPARα were up-regulated by esculetin and those of SREBP-1c were down-regulated. Esculetin elevated expression of hepatic fatty acid oxidation genes (PGC-1α, PPARα, Acsl1 and CPT), while suppressing expression of fatty acid synthesis gene. Anti-insulin resistant effects of esculetin were mediated by increased hepatic GLUT2 and glucokinase mRNA levels and decreased glucose-6-phosphatase mRNA level. These findings suggest that esculetin supplementation has beneficial effects on hepatosteatosis and insulin resistance in HFD-induced obese mice, partly via activation of the AdiopR2–AMPK pathway. |
| first_indexed | 2024-12-24T04:24:07Z |
| format | Article |
| id | doaj.art-9605479db06a4159840ca6203d3bda31 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-24T04:24:07Z |
| publishDate | 2015-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-9605479db06a4159840ca6203d3bda312022-12-21T17:15:42ZengElsevierJournal of Functional Foods1756-46462015-05-0115160171Long-term supplementation of esculetin ameliorates hepatosteatosis and insulin resistance partly by activating AdipoR2–AMPK pathway in diet-induced obese miceMi-Ok Sim0Hae-In Lee1Ju Ri Ham2Kwon-Il Seo3Mi-Kyung Lee4Jeollanamdo Development Institute of Korean Traditional Medicine, Jangheung 529-851, Republic of KoreaDepartment of Food and Nutrition, Sunchon National University, Suncheon 540-950, Republic of KoreaDepartment of Food and Nutrition, Sunchon National University, Suncheon 540-950, Republic of KoreaDepartment of Food and Nutrition, Sunchon National University, Suncheon 540-950, Republic of KoreaDepartment of Food and Nutrition, Sunchon National University, Suncheon 540-950, Republic of Korea; Corresponding author. Department of Food and Nutrition, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 540-950, Republic of Korea. Tel.: +82 61 750 3656; fax: +82 61 752 3657.The effects of esculetin on hepatosteatosis and insulin resistance in high-fat diet (HFD)-induced obese mice were investigated. Esculetin (0.02%, w/w) provided to mice fed a HFD (40% kcal from fat) for 12 weeks reduced body weight gain, visceral adiposity, hepatosteatosis, hyperlipidemia and hyperglycemia, whereas it increased plasma adiponectin level and the protein and mRNA expression of hepatic AdipoR2, which led to the activation of AMPK. The protein and mRNA expression of hepatic PPARα were up-regulated by esculetin and those of SREBP-1c were down-regulated. Esculetin elevated expression of hepatic fatty acid oxidation genes (PGC-1α, PPARα, Acsl1 and CPT), while suppressing expression of fatty acid synthesis gene. Anti-insulin resistant effects of esculetin were mediated by increased hepatic GLUT2 and glucokinase mRNA levels and decreased glucose-6-phosphatase mRNA level. These findings suggest that esculetin supplementation has beneficial effects on hepatosteatosis and insulin resistance in HFD-induced obese mice, partly via activation of the AdiopR2–AMPK pathway.http://www.sciencedirect.com/science/article/pii/S175646461500122XEsculetinInsulin resistanceLiverObesitySteatosis |
| spellingShingle | Mi-Ok Sim Hae-In Lee Ju Ri Ham Kwon-Il Seo Mi-Kyung Lee Long-term supplementation of esculetin ameliorates hepatosteatosis and insulin resistance partly by activating AdipoR2–AMPK pathway in diet-induced obese mice Journal of Functional Foods Esculetin Insulin resistance Liver Obesity Steatosis |
| title | Long-term supplementation of esculetin ameliorates hepatosteatosis and insulin resistance partly by activating AdipoR2–AMPK pathway in diet-induced obese mice |
| title_full | Long-term supplementation of esculetin ameliorates hepatosteatosis and insulin resistance partly by activating AdipoR2–AMPK pathway in diet-induced obese mice |
| title_fullStr | Long-term supplementation of esculetin ameliorates hepatosteatosis and insulin resistance partly by activating AdipoR2–AMPK pathway in diet-induced obese mice |
| title_full_unstemmed | Long-term supplementation of esculetin ameliorates hepatosteatosis and insulin resistance partly by activating AdipoR2–AMPK pathway in diet-induced obese mice |
| title_short | Long-term supplementation of esculetin ameliorates hepatosteatosis and insulin resistance partly by activating AdipoR2–AMPK pathway in diet-induced obese mice |
| title_sort | long term supplementation of esculetin ameliorates hepatosteatosis and insulin resistance partly by activating adipor2 ampk pathway in diet induced obese mice |
| topic | Esculetin Insulin resistance Liver Obesity Steatosis |
| url | http://www.sciencedirect.com/science/article/pii/S175646461500122X |
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