Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran
Background: Dogs, as the definitive host of Neospora caninum, are important in the epidemiology of this parasitic infection. We aimed to determine the prevalence of N. caninum infection in a dog population from a rural setting in Fars Province, Southern Iran, using a combination of molecular and ser...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2021-02-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2795 |
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| author | Morvarid HARIRI Nasir AREFKHAH Fariba GHORBANI Mehdi NAMAVARI Mostafa OMIDIAN Bahador SARKARI |
| author_facet | Morvarid HARIRI Nasir AREFKHAH Fariba GHORBANI Mehdi NAMAVARI Mostafa OMIDIAN Bahador SARKARI |
| author_sort | Morvarid HARIRI |
| collection | DOAJ |
| description | Background: Dogs, as the definitive host of Neospora caninum, are important in the epidemiology of this parasitic infection. We aimed to determine the prevalence of N. caninum infection in a dog population from a rural setting in Fars Province, Southern Iran, using a combination of molecular and serological techniques.
Methods: This cross-sectional study was carried out in Nov 2018 in three rural districts, Sar Mashhad, HosseinAbad, and Tolesaman located in Kazeroun Township in Fars province, southern Iran. Blood samples were taken from 60 stray and household dogs. Dogs’ sera were tested for antibodies against N. caninum, using a Neospora-Modified Agglutination Test. Moreover, dogs’ buffy coats were tested for Neospora DNA, using a molecular method.
Results: Anti-Neospora antibodies were detected in sera of 4 out of 60 dogs, corresponding to a seroprevalence rate of 6.7%. Out of 25 female dogs, 1 was seropositive and of 35 males, 3 were seropositive, yet the differences were not statistically significant. The infection was more prevalent in adult dogs (> 12 months), nevertheless, the differences between age and Neospora seropositivity was not statistically significant. N. caninum DNA was not detected in the buffy coat of any of the studied dogs.
Conclusion: Findings of the study indicate that N. caninum is a common infection in dogs in rural areas of Fars province in southern Iran. The infected dogs might be a potentially important source of N. caninum infection to livestock in the area. |
| first_indexed | 2024-12-17T07:40:40Z |
| format | Article |
| id | doaj.art-96099fa4ff6c48f19b547061186b424a |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-17T07:40:40Z |
| publishDate | 2021-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-96099fa4ff6c48f19b547061186b424a2022-12-21T21:58:09ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2021-02-011611461502795Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern IranMorvarid HARIRI0Nasir AREFKHAH1Fariba GHORBANI2Mehdi NAMAVARI3Mostafa OMIDIAN4Bahador SARKARI51. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran2. Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran3. Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organi-zation (AREEO), Shiraz, Iran1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, IranBackground: Dogs, as the definitive host of Neospora caninum, are important in the epidemiology of this parasitic infection. We aimed to determine the prevalence of N. caninum infection in a dog population from a rural setting in Fars Province, Southern Iran, using a combination of molecular and serological techniques. Methods: This cross-sectional study was carried out in Nov 2018 in three rural districts, Sar Mashhad, HosseinAbad, and Tolesaman located in Kazeroun Township in Fars province, southern Iran. Blood samples were taken from 60 stray and household dogs. Dogs’ sera were tested for antibodies against N. caninum, using a Neospora-Modified Agglutination Test. Moreover, dogs’ buffy coats were tested for Neospora DNA, using a molecular method. Results: Anti-Neospora antibodies were detected in sera of 4 out of 60 dogs, corresponding to a seroprevalence rate of 6.7%. Out of 25 female dogs, 1 was seropositive and of 35 males, 3 were seropositive, yet the differences were not statistically significant. The infection was more prevalent in adult dogs (> 12 months), nevertheless, the differences between age and Neospora seropositivity was not statistically significant. N. caninum DNA was not detected in the buffy coat of any of the studied dogs. Conclusion: Findings of the study indicate that N. caninum is a common infection in dogs in rural areas of Fars province in southern Iran. The infected dogs might be a potentially important source of N. caninum infection to livestock in the area.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2795seroprevalenceneospora caninumdogsiran |
| spellingShingle | Morvarid HARIRI Nasir AREFKHAH Fariba GHORBANI Mehdi NAMAVARI Mostafa OMIDIAN Bahador SARKARI Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran Iranian Journal of Parasitology seroprevalence neospora caninum dogs iran |
| title | Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran |
| title_full | Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran |
| title_fullStr | Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran |
| title_full_unstemmed | Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran |
| title_short | Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran |
| title_sort | molecular and serological evaluation of neospora caninum infec tion in dogs from a rural setting in fars province southern iran |
| topic | seroprevalence neospora caninum dogs iran |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2795 |
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