The Effects of Systemic Vitamin D Level on the Healing of Different Graft Materials: An Experimental Histological Study
The aim of this study is to investigate the effects of serum vitamin D levels on the healing of different bone graft materials. Thirty-six male rats were divided into three groups and fed special feeds containing different amounts of vitamin D for 6 weeks before the surgical phase: the high serum vi...
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MDPI AG
2024-01-01
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| author | Mehmet Sefa Hacibektasoglu Huseyin Avni Balcioglu Yigit Uyanikgil Nilufer Bolukbasi Balcioglu |
| author_facet | Mehmet Sefa Hacibektasoglu Huseyin Avni Balcioglu Yigit Uyanikgil Nilufer Bolukbasi Balcioglu |
| author_sort | Mehmet Sefa Hacibektasoglu |
| collection | DOAJ |
| description | The aim of this study is to investigate the effects of serum vitamin D levels on the healing of different bone graft materials. Thirty-six male rats were divided into three groups and fed special feeds containing different amounts of vitamin D for 6 weeks before the surgical phase: the high serum vitamin D level group (group H) 10,000 iu/kg vitamin D3; the standard serum vitamin D level group (group C) 1000 iu/kg D3; and the low-level vitamin D group (group L) 100 iu/kg vitamin D3. Under general anesthesia, four defects with a diameter of 5 mm were created in the calvaria of the rats. The defects were augmented with autogenous grafts, allografts, xenografts, or left empty. The serum vitamin D level was measured before the surgery and before sacrifice. At the end of the 6th week, the subjects were sacrificed, and histological and histomorphometric analyses were performed. Study results show that in all graft types, as vitamin D levels increase, the number of new bone formations increases. There was no significant difference between the graft materials in terms of new bone formation criteria in group L. In group H and group C, the highest new bone formation was seen in the allograft group (1.48 ± 0.07, 0.66 ± 0.19, respectively). Prospective randomized clinical studies are required to evaluate the effect of vitamin D dose on the success of augmentation procedures in the clinic. |
| first_indexed | 2024-03-08T09:57:59Z |
| format | Article |
| id | doaj.art-960bdd9250e6451ca2a62aa8b57e4f7a |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-08T09:57:59Z |
| publishDate | 2024-01-01 |
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| series | Applied Sciences |
| spelling | doaj.art-960bdd9250e6451ca2a62aa8b57e4f7a2024-01-29T13:46:11ZengMDPI AGApplied Sciences2076-34172024-01-0114293910.3390/app14020939The Effects of Systemic Vitamin D Level on the Healing of Different Graft Materials: An Experimental Histological StudyMehmet Sefa Hacibektasoglu0Huseyin Avni Balcioglu1Yigit Uyanikgil2Nilufer Bolukbasi Balcioglu3Department of Oral Implantology, Faculty of Dentistry, Istanbul University, Istanbul 34093, TurkeyDepartment of Anatomy, Faculty of Medicine, Health Sciences University, Istanbul 34716, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Ege University, Izmir 35030, TurkeyDepartment of Oral Implantology, Faculty of Dentistry, Istanbul University, Istanbul 34093, TurkeyThe aim of this study is to investigate the effects of serum vitamin D levels on the healing of different bone graft materials. Thirty-six male rats were divided into three groups and fed special feeds containing different amounts of vitamin D for 6 weeks before the surgical phase: the high serum vitamin D level group (group H) 10,000 iu/kg vitamin D3; the standard serum vitamin D level group (group C) 1000 iu/kg D3; and the low-level vitamin D group (group L) 100 iu/kg vitamin D3. Under general anesthesia, four defects with a diameter of 5 mm were created in the calvaria of the rats. The defects were augmented with autogenous grafts, allografts, xenografts, or left empty. The serum vitamin D level was measured before the surgery and before sacrifice. At the end of the 6th week, the subjects were sacrificed, and histological and histomorphometric analyses were performed. Study results show that in all graft types, as vitamin D levels increase, the number of new bone formations increases. There was no significant difference between the graft materials in terms of new bone formation criteria in group L. In group H and group C, the highest new bone formation was seen in the allograft group (1.48 ± 0.07, 0.66 ± 0.19, respectively). Prospective randomized clinical studies are required to evaluate the effect of vitamin D dose on the success of augmentation procedures in the clinic.https://www.mdpi.com/2076-3417/14/2/939vitamin Dbone graftsbone defectnew bone formationrat calvariaanimal model |
| spellingShingle | Mehmet Sefa Hacibektasoglu Huseyin Avni Balcioglu Yigit Uyanikgil Nilufer Bolukbasi Balcioglu The Effects of Systemic Vitamin D Level on the Healing of Different Graft Materials: An Experimental Histological Study Applied Sciences vitamin D bone grafts bone defect new bone formation rat calvaria animal model |
| title | The Effects of Systemic Vitamin D Level on the Healing of Different Graft Materials: An Experimental Histological Study |
| title_full | The Effects of Systemic Vitamin D Level on the Healing of Different Graft Materials: An Experimental Histological Study |
| title_fullStr | The Effects of Systemic Vitamin D Level on the Healing of Different Graft Materials: An Experimental Histological Study |
| title_full_unstemmed | The Effects of Systemic Vitamin D Level on the Healing of Different Graft Materials: An Experimental Histological Study |
| title_short | The Effects of Systemic Vitamin D Level on the Healing of Different Graft Materials: An Experimental Histological Study |
| title_sort | effects of systemic vitamin d level on the healing of different graft materials an experimental histological study |
| topic | vitamin D bone grafts bone defect new bone formation rat calvaria animal model |
| url | https://www.mdpi.com/2076-3417/14/2/939 |
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