NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis
Skin avulsion is commonly seen in individuals exposed to heavy shearing forces. Subcutaneous tissue detachment and bone fractures usually accompany skin avulsion. Thus, the estimation of the extent of damaged tissue is very important. Currently, the viability of skin and subcutaneous tissue is deter...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fchem.2019.00696/full |
| _version_ | 1818367624113815552 |
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| author | Yizhou Li Xiang Hu Wanrong Yi Daifeng Li Yaqi Guo Baiwen Qi Aixi Yu |
| author_facet | Yizhou Li Xiang Hu Wanrong Yi Daifeng Li Yaqi Guo Baiwen Qi Aixi Yu |
| author_sort | Yizhou Li |
| collection | DOAJ |
| description | Skin avulsion is commonly seen in individuals exposed to heavy shearing forces. Subcutaneous tissue detachment and bone fractures usually accompany skin avulsion. Thus, the estimation of the extent of damaged tissue is very important. Currently, the viability of skin and subcutaneous tissue is determined by clinical observations, and these observations always underestimate the true extent of the avulsed skin. Herein, we synthesized an innovative probe, CH1055-GRRRDEVDK (CH1055-GK), which can specifically bind to caspase-3 so as to image skin avulsion and define necrotic regions. Our uptake and binding affinity tests in apoptotic cells and evaluation of the probe ex vivo and in vivo showed that the probe has a strong ability to bind caspase-3 in skin avulsion models and that it vividly detected the necrotic area in avulsed skins. Furthermore, the increased fluorescence intensity of the probe in the avulsed skin showed a larger affected area than that determined by clinical observations in live mice. Consequently, our results indicated that observation of the caspase-3-targeted probe CH1055-GK via NIR-II imaging allowed the clear detection of skin avulsion in subjects, indicating its potential as an imaging tool for the early diagnosis of skin avulsion and the determination of necrotic margins. |
| first_indexed | 2024-12-13T22:55:00Z |
| format | Article |
| id | doaj.art-960c7ebd3092448eb49cb752a399cd93 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-13T22:55:00Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-960c7ebd3092448eb49cb752a399cd932022-12-21T23:28:31ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462019-10-01710.3389/fchem.2019.00696486559NIR-II Fluorescence Imaging of Skin Avulsion and NecrosisYizhou LiXiang HuWanrong YiDaifeng LiYaqi GuoBaiwen QiAixi YuSkin avulsion is commonly seen in individuals exposed to heavy shearing forces. Subcutaneous tissue detachment and bone fractures usually accompany skin avulsion. Thus, the estimation of the extent of damaged tissue is very important. Currently, the viability of skin and subcutaneous tissue is determined by clinical observations, and these observations always underestimate the true extent of the avulsed skin. Herein, we synthesized an innovative probe, CH1055-GRRRDEVDK (CH1055-GK), which can specifically bind to caspase-3 so as to image skin avulsion and define necrotic regions. Our uptake and binding affinity tests in apoptotic cells and evaluation of the probe ex vivo and in vivo showed that the probe has a strong ability to bind caspase-3 in skin avulsion models and that it vividly detected the necrotic area in avulsed skins. Furthermore, the increased fluorescence intensity of the probe in the avulsed skin showed a larger affected area than that determined by clinical observations in live mice. Consequently, our results indicated that observation of the caspase-3-targeted probe CH1055-GK via NIR-II imaging allowed the clear detection of skin avulsion in subjects, indicating its potential as an imaging tool for the early diagnosis of skin avulsion and the determination of necrotic margins.https://www.frontiersin.org/article/10.3389/fchem.2019.00696/fullNIR-II fluorescence imagingskin avulsionnecrosisapoptotic cellscaspase-3 |
| spellingShingle | Yizhou Li Xiang Hu Wanrong Yi Daifeng Li Yaqi Guo Baiwen Qi Aixi Yu NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis Frontiers in Chemistry NIR-II fluorescence imaging skin avulsion necrosis apoptotic cells caspase-3 |
| title | NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis |
| title_full | NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis |
| title_fullStr | NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis |
| title_full_unstemmed | NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis |
| title_short | NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis |
| title_sort | nir ii fluorescence imaging of skin avulsion and necrosis |
| topic | NIR-II fluorescence imaging skin avulsion necrosis apoptotic cells caspase-3 |
| url | https://www.frontiersin.org/article/10.3389/fchem.2019.00696/full |
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